The refined crystal structure of toxic shock syndrome toxin-1 at 2.07 Å resolution

Anastassios C. Papageorgiou, Rossalyn D. Brehm, Demetrios D. Leonidas, Howard S. Tranter, K. Ravi Acharya

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The pyrogenic toxin toxic shock syndrome toxin-1 from Staphylococcus aureus is a causative agent of the toxic shock syndrome disease. It belongs to a family of proteins known as superantigens that cross-link major histocompatibility class II molecules and T-cell receptors leading to the activation of a substantial number of T cells. The crystal structure of this protein has been refined to 2.07 Å with an R(cryst) value of 20.4% for 51,240 reflections. The final model contains three molecules in the asymmetric unit with good stereochemistry and a root-mean-square deviation of 0.009 Å and 1.63° from ideality for bond lengths and bond angles, respectively. The overall fold is considerably similar to that of other known microbial superantigens (staphylococcal enterotoxins). However, a detailed structural analysis shows that toxic shock syndrome toxin-1 lacks several structural features that affect its specificity for Vβ elements of the T-cell receptor and also its recognition by major histocompatibility class II molecules.

Original languageEnglish
Pages (from-to)553-569
Number of pages17
JournalJournal of Molecular Biology
Volume260
Issue number4
DOIs
Publication statusPublished - 26 Jul 1996

Keywords

  • Major histocompatibility complex
  • Superantigens
  • T-cell receptor
  • Toxic shock syndrome
  • X-ray crystallography

ASJC Scopus subject areas

  • Molecular Biology

Cite this

The refined crystal structure of toxic shock syndrome toxin-1 at 2.07 Å resolution. / Papageorgiou, Anastassios C.; Brehm, Rossalyn D.; Leonidas, Demetrios D.; Tranter, Howard S.; Acharya, K. Ravi.

In: Journal of Molecular Biology, Vol. 260, No. 4, 26.07.1996, p. 553-569.

Research output: Contribution to journalArticle

Papageorgiou, Anastassios C. ; Brehm, Rossalyn D. ; Leonidas, Demetrios D. ; Tranter, Howard S. ; Acharya, K. Ravi. / The refined crystal structure of toxic shock syndrome toxin-1 at 2.07 Å resolution. In: Journal of Molecular Biology. 1996 ; Vol. 260, No. 4. pp. 553-569.
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