Abstract
The pyrogenic toxin toxic shock syndrome toxin-1 from Staphylococcus aureus is a causative agent of the toxic shock syndrome disease. It belongs to a family of proteins known as superantigens that cross-link major histocompatibility class II molecules and T-cell receptors leading to the activation of a substantial number of T cells. The crystal structure of this protein has been refined to 2.07 Å with an R(cryst) value of 20.4% for 51,240 reflections. The final model contains three molecules in the asymmetric unit with good stereochemistry and a root-mean-square deviation of 0.009 Å and 1.63° from ideality for bond lengths and bond angles, respectively. The overall fold is considerably similar to that of other known microbial superantigens (staphylococcal enterotoxins). However, a detailed structural analysis shows that toxic shock syndrome toxin-1 lacks several structural features that affect its specificity for Vβ elements of the T-cell receptor and also its recognition by major histocompatibility class II molecules.
| Original language | English |
|---|---|
| Pages (from-to) | 553-569 |
| Number of pages | 17 |
| Journal | Journal of Molecular Biology |
| Volume | 260 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 26 Jul 1996 |
Keywords
- Major histocompatibility complex
- Superantigens
- T-cell receptor
- Toxic shock syndrome
- X-ray crystallography
ASJC Scopus subject areas
- Molecular Biology
Cite this
The refined crystal structure of toxic shock syndrome toxin-1 at 2.07 Å resolution. / Papageorgiou, Anastassios C.; Brehm, Rossalyn D.; Leonidas, Demetrios D.; Tranter, Howard S.; Acharya, K. Ravi.
In: Journal of Molecular Biology, Vol. 260, No. 4, 26.07.1996, p. 553-569.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The refined crystal structure of toxic shock syndrome toxin-1 at 2.07 Å resolution
AU - Papageorgiou, Anastassios C.
AU - Brehm, Rossalyn D.
AU - Leonidas, Demetrios D.
AU - Tranter, Howard S.
AU - Acharya, K. Ravi
PY - 1996/7/26
Y1 - 1996/7/26
N2 - The pyrogenic toxin toxic shock syndrome toxin-1 from Staphylococcus aureus is a causative agent of the toxic shock syndrome disease. It belongs to a family of proteins known as superantigens that cross-link major histocompatibility class II molecules and T-cell receptors leading to the activation of a substantial number of T cells. The crystal structure of this protein has been refined to 2.07 Å with an R(cryst) value of 20.4% for 51,240 reflections. The final model contains three molecules in the asymmetric unit with good stereochemistry and a root-mean-square deviation of 0.009 Å and 1.63° from ideality for bond lengths and bond angles, respectively. The overall fold is considerably similar to that of other known microbial superantigens (staphylococcal enterotoxins). However, a detailed structural analysis shows that toxic shock syndrome toxin-1 lacks several structural features that affect its specificity for Vβ elements of the T-cell receptor and also its recognition by major histocompatibility class II molecules.
AB - The pyrogenic toxin toxic shock syndrome toxin-1 from Staphylococcus aureus is a causative agent of the toxic shock syndrome disease. It belongs to a family of proteins known as superantigens that cross-link major histocompatibility class II molecules and T-cell receptors leading to the activation of a substantial number of T cells. The crystal structure of this protein has been refined to 2.07 Å with an R(cryst) value of 20.4% for 51,240 reflections. The final model contains three molecules in the asymmetric unit with good stereochemistry and a root-mean-square deviation of 0.009 Å and 1.63° from ideality for bond lengths and bond angles, respectively. The overall fold is considerably similar to that of other known microbial superantigens (staphylococcal enterotoxins). However, a detailed structural analysis shows that toxic shock syndrome toxin-1 lacks several structural features that affect its specificity for Vβ elements of the T-cell receptor and also its recognition by major histocompatibility class II molecules.
KW - Major histocompatibility complex
KW - Superantigens
KW - T-cell receptor
KW - Toxic shock syndrome
KW - X-ray crystallography
UR - http://www.scopus.com/inward/record.url?scp=0030602908&partnerID=8YFLogxK
U2 - 10.1006/jmbi.1996.0421
DO - 10.1006/jmbi.1996.0421
M3 - Article
VL - 260
SP - 553
EP - 569
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
SN - 0022-2836
IS - 4
ER -