The putative flavin carrier family FLcA-C is important for Aspergillus fumigatus virulence

Patrícia A. de Castro, Jéssica Chiaratto, Enyara Rezende Morais, Thaila Fernanda dos Reis, Thomas K. Mitchell, Neil A. Brown, Gustavo H. Goldman

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Aspergillus fumigatus is an opportunistic fungal pathogen and the most important species causing pulmonary fungal infections. The signaling by calcium is very important for A. fumigatus pathogenicity and it is regulated by the transcription factor CrzA. We have previously used used ChIP-seq (Chromatin Immunoprecipitation DNA sequencing) aiming to identify gene targets regulated by CrzA. We have identified among several genes regulated by calcium stress, the putative flavin transporter, flcA. This transporter belongs to a small protein family composed of FlcA, B, and C. The ∆flcA null mutant showed several phenotypes, such as morphological defects, increased sensitivity to calcium chelating-agent ethylene glycol tetraacetic acid (EGTA), cell wall or oxidative damaging agents and metals, repre-sentative of deficiencies in calcium signaling and iron homeostasis. Increasing calcium concentrations improved significantly the ∆flcA growth and conidiation, indicating that ∆flcA mutant has calcium insufficiency. Finally, ∆flcA-C mutants showed reduced flavin adenine dinucleotide (FAD) and were avirulent in a low dose murine infection model.

Original languageEnglish
Pages (from-to)797-809
Number of pages13
JournalVirulence
Volume8
Issue number6
Early online date1 Nov 2016
DOIs
Publication statusPublished - 14 Oct 2017

Keywords

  • Aspergillus fumigatus
  • Calcium
  • CrzA
  • FAD metabolism
  • Putative flavin flcA-C transporter family

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

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    de Castro, P. A., Chiaratto, J., Morais, E. R., dos Reis, T. F., Mitchell, T. K., Brown, N. A., & Goldman, G. H. (2017). The putative flavin carrier family FLcA-C is important for Aspergillus fumigatus virulence. Virulence, 8(6), 797-809. https://doi.org/10.1080/21505594.2016.1239010