The MDCK variety pack: choosing the right strain

Research output: Contribution to journalArticle

98 Citations (Scopus)
72 Downloads (Pure)

Abstract

The MDCK cell line provides a tractable model for studying protein trafficking, polarity and junctions (tight, adherens, desmosome and gap) in epithelial cells. However, there are many different strains of MDCK cells available, including the parental line, MDCK I, MDCK II, MDCK. 1, MDCK. 2, superdome and supertube, making it difficult for new researchers to decide which strain to use. Furthermore, there is often inadequate reporting of strain types and where cells were obtained from in the literature. This review aims to provide new researchers with a guide to the different MDCK strains and a directory of where they can be obtained. We also hope to encourage experienced researchers to report the stain and origin of their MDCK cells.
Original languageEnglish
Article number43
JournalBMC Cell Biology
Volume12
DOIs
Publication statusPublished - 7 Oct 2011

Fingerprint

Madin Darby Canine Kidney Cells
Research Personnel
Adherens Junctions
Directories
Desmosomes
Tight Junctions
Gap Junctions
Protein Transport
Coloring Agents
Epithelial Cells
Cell Line

Cite this

The MDCK variety pack: choosing the right strain. / Dukes, Joseph D; Whitley, Paul; Chalmers, Andrew D.

In: BMC Cell Biology, Vol. 12, 43, 07.10.2011.

Research output: Contribution to journalArticle

@article{8e7b84052d634bd5b96c713a264a8e6a,
title = "The MDCK variety pack: choosing the right strain",
abstract = "The MDCK cell line provides a tractable model for studying protein trafficking, polarity and junctions (tight, adherens, desmosome and gap) in epithelial cells. However, there are many different strains of MDCK cells available, including the parental line, MDCK I, MDCK II, MDCK. 1, MDCK. 2, superdome and supertube, making it difficult for new researchers to decide which strain to use. Furthermore, there is often inadequate reporting of strain types and where cells were obtained from in the literature. This review aims to provide new researchers with a guide to the different MDCK strains and a directory of where they can be obtained. We also hope to encourage experienced researchers to report the stain and origin of their MDCK cells.",
author = "Dukes, {Joseph D} and Paul Whitley and Chalmers, {Andrew D}",
year = "2011",
month = "10",
day = "7",
doi = "10.1186/1471-2121-12-43",
language = "English",
volume = "12",
journal = "BMC Cell Biology",
issn = "1471-2121",
publisher = "BioMed Central",

}

TY - JOUR

T1 - The MDCK variety pack: choosing the right strain

AU - Dukes, Joseph D

AU - Whitley, Paul

AU - Chalmers, Andrew D

PY - 2011/10/7

Y1 - 2011/10/7

N2 - The MDCK cell line provides a tractable model for studying protein trafficking, polarity and junctions (tight, adherens, desmosome and gap) in epithelial cells. However, there are many different strains of MDCK cells available, including the parental line, MDCK I, MDCK II, MDCK. 1, MDCK. 2, superdome and supertube, making it difficult for new researchers to decide which strain to use. Furthermore, there is often inadequate reporting of strain types and where cells were obtained from in the literature. This review aims to provide new researchers with a guide to the different MDCK strains and a directory of where they can be obtained. We also hope to encourage experienced researchers to report the stain and origin of their MDCK cells.

AB - The MDCK cell line provides a tractable model for studying protein trafficking, polarity and junctions (tight, adherens, desmosome and gap) in epithelial cells. However, there are many different strains of MDCK cells available, including the parental line, MDCK I, MDCK II, MDCK. 1, MDCK. 2, superdome and supertube, making it difficult for new researchers to decide which strain to use. Furthermore, there is often inadequate reporting of strain types and where cells were obtained from in the literature. This review aims to provide new researchers with a guide to the different MDCK strains and a directory of where they can be obtained. We also hope to encourage experienced researchers to report the stain and origin of their MDCK cells.

UR - http://www.scopus.com/inward/record.url?scp=80053554604&partnerID=8YFLogxK

UR - http://dx.doi.org/10.1186/1471-2121-12-43

U2 - 10.1186/1471-2121-12-43

DO - 10.1186/1471-2121-12-43

M3 - Article

VL - 12

JO - BMC Cell Biology

JF - BMC Cell Biology

SN - 1471-2121

M1 - 43

ER -