The maternally expressed WRKY transcription factor TTG2 Controls lethality in interploidy crosses of Arabidopsis

Brian P Dilkes, Melissa Spielman, Renate Weizbauer, Brian Watson, Diana Burkart-Waco, Rod J Scott, Luca Comai

Research output: Contribution to journalArticle

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Abstract

The molecular mechanisms underlying lethality of F1 hybrids between diverged parents are one target of speciation research. Crosses between diploid and tetraploid individuals of the same genotype can result in F1 lethality, and this dosage-sensitive incompatibility plays a role in polyploid speciation. We have identified variation in F1 lethality in interploidy crosses of Arabidopsis thaliana and determined the genetic architecture of the maternally expressed variation via QTL mapping. A single large-effect QTL, DR. STRANGELOVE 1 (DSL1), was identified as well as two QTL with epistatic relationships to DSL1. DSL1 affects the rate of postzygotic lethality via expression in the maternal sporophyte. Fine mapping placed DSL1 in an interval encoding the maternal effect transcription factor TTG2. Maternal parents carrying loss-of-function mutations in TTG2 suppressed the F1 lethality caused by paternal excess interploidy crosses. The frequency of cellularization in the endosperm was similarly affected by both natural variation and ttg2 loss-of-function mutants. The simple genetic basis of the natural variation and effects of single-gene mutations suggests that F1 lethality in polyploids could evolve rapidly. Furthermore, the role of the sporophytically active TTG2 gene in interploidy crosses indicates that the developmental programming of the mother regulates the viability of interploidy hybrid offspring.
LanguageEnglish
Article numbere308
Pages2707-2720
Number of pages14
JournalPLoS Biology
Volume6
Issue number12
DOIs
StatusPublished - 8 Dec 2008

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Polyploidy
Arabidopsis
Transcription Factors
transcription factors
Genes
Mothers
quantitative trait loci
Endosperm
Mutation
Tetraploidy
Diploidy
polyploidy
Genotype
maternal effect
Research
tetraploidy
endosperm
diploidy
genes
Arabidopsis thaliana

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The maternally expressed WRKY transcription factor TTG2 Controls lethality in interploidy crosses of Arabidopsis. / Dilkes, Brian P; Spielman, Melissa; Weizbauer, Renate; Watson, Brian; Burkart-Waco, Diana; Scott, Rod J; Comai, Luca.

In: PLoS Biology, Vol. 6, No. 12, e308, 08.12.2008, p. 2707-2720.

Research output: Contribution to journalArticle

Dilkes, BP, Spielman, M, Weizbauer, R, Watson, B, Burkart-Waco, D, Scott, RJ & Comai, L 2008, 'The maternally expressed WRKY transcription factor TTG2 Controls lethality in interploidy crosses of Arabidopsis' PLoS Biology, vol. 6, no. 12, e308, pp. 2707-2720. DOI: 10.1371/journal.pbio.0060308
Dilkes BP, Spielman M, Weizbauer R, Watson B, Burkart-Waco D, Scott RJ et al. The maternally expressed WRKY transcription factor TTG2 Controls lethality in interploidy crosses of Arabidopsis. PLoS Biology. 2008 Dec 8;6(12):2707-2720. e308. Available from, DOI: 10.1371/journal.pbio.0060308
Dilkes, Brian P ; Spielman, Melissa ; Weizbauer, Renate ; Watson, Brian ; Burkart-Waco, Diana ; Scott, Rod J ; Comai, Luca. / The maternally expressed WRKY transcription factor TTG2 Controls lethality in interploidy crosses of Arabidopsis. In: PLoS Biology. 2008 ; Vol. 6, No. 12. pp. 2707-2720
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