TY - JOUR
T1 - The MAP kinase inhibitors, PD098059, UO126 and SB203580, inhibit IL-1β-dependent PGE2 release via mechanistically distinct processes
AU - Robert, Newton
AU - Cambridge, Lisa
AU - Hart, Lorraine A.
AU - Stevens, David A.
AU - Lindsay, Mark A.
AU - Barnes, Peter J.
PY - 2000/7/31
Y1 - 2000/7/31
N2 - 1. In common with human bronchial epithelial cells, pulmonary A549 cells release prostaglandin (PG) E2 in response to pro-inflammatory cytokines. We have therefore used these cells to examine the effect of the selective mitogen activated protein (MAP) kinase inhibitors; PD098059, a mitogen activated and extracellular regulated kinase kinase (MEK) 1 inhibitor, UO126, a dual MEK1 and MEK2 inhibitor, and SB203580, a p38 MAP kinase inhibitor in the IL-1β-dependent release of PGE2. 2. Following IL-1β treatment the extracellular regulated kinases (ERKs) and the p38 MAP kinases were rapidly phosphorylated. 3. PD09059, UO126 and SB203580 prevented IL-1β-induced PGE2 release at doses that correlated closely with published IC50 values. Small or partial effects at the relevant doses were observed on induction of cyclo-oxygenase (COX) activity or COX-2 protein suggesting that the primary effects were at the level of arachidonate availability. 4. Neither PD098059 nor SB203580 showed any effect on IL-1β-induced arachidonate release. We therefore speculate that the MEK1/ERK and p38 kinase cascades play a role in the functional coupling of arachidonate release to COX-2. 5. In contrast, UO126 was highly effective at inhibiting IL-1β-dependent arachidonate release, implicating MEK2 in the activation of the PLA2 that is involved in IL-1β-dependent PGE2 release. 6. We conclude that the MEK1, MEK2 and p38 MAP kinase inhibitors, PD098059, UO126 and SB203580, are highly potent in respect of inflammatory PG release. Finally, we conclude that these inhibitors act via mechanistically distinct processes, which may have anti-inflammatory benefits.
AB - 1. In common with human bronchial epithelial cells, pulmonary A549 cells release prostaglandin (PG) E2 in response to pro-inflammatory cytokines. We have therefore used these cells to examine the effect of the selective mitogen activated protein (MAP) kinase inhibitors; PD098059, a mitogen activated and extracellular regulated kinase kinase (MEK) 1 inhibitor, UO126, a dual MEK1 and MEK2 inhibitor, and SB203580, a p38 MAP kinase inhibitor in the IL-1β-dependent release of PGE2. 2. Following IL-1β treatment the extracellular regulated kinases (ERKs) and the p38 MAP kinases were rapidly phosphorylated. 3. PD09059, UO126 and SB203580 prevented IL-1β-induced PGE2 release at doses that correlated closely with published IC50 values. Small or partial effects at the relevant doses were observed on induction of cyclo-oxygenase (COX) activity or COX-2 protein suggesting that the primary effects were at the level of arachidonate availability. 4. Neither PD098059 nor SB203580 showed any effect on IL-1β-induced arachidonate release. We therefore speculate that the MEK1/ERK and p38 kinase cascades play a role in the functional coupling of arachidonate release to COX-2. 5. In contrast, UO126 was highly effective at inhibiting IL-1β-dependent arachidonate release, implicating MEK2 in the activation of the PLA2 that is involved in IL-1β-dependent PGE2 release. 6. We conclude that the MEK1, MEK2 and p38 MAP kinase inhibitors, PD098059, UO126 and SB203580, are highly potent in respect of inflammatory PG release. Finally, we conclude that these inhibitors act via mechanistically distinct processes, which may have anti-inflammatory benefits.
KW - Epithelial cell
KW - MAP kinase
KW - Prostaglandin GH synthase
UR - http://www.scopus.com/inward/record.url?scp=0033914594&partnerID=8YFLogxK
U2 - 10.1038/sj.bjp.0703431
DO - 10.1038/sj.bjp.0703431
M3 - Article
C2 - 10903976
AN - SCOPUS:0033914594
SN - 0007-1188
VL - 130
SP - 1353
EP - 1361
JO - British Journal of Pharmacology
JF - British Journal of Pharmacology
IS - 6
ER -