5 Citations (SciVal)


Adipose tissue and physical inactivity both influence metabolic health and systemic inflammation, but how adipose tissue responds to chronic physical inactivity is unknown.

This work aimed to characterize the impact of chronic physical inactivity on adipose tissue in healthy, young males.

We collected subcutaneous adipose tissue from 20 healthy, young men before and after 60 days of complete bed rest with energy intake reduced to maintain energy balance and fat mass. We used RNA sequencing, flow cytometry, ex vivo tissue culture, and targeted protein analyses to examine adipose tissue phenotype.

Our results indicate that the adipose tissue transcriptome, stromal cellular compartment, and insulin signaling protein abundance are largely unaffected by bed rest when fat mass is kept stable. However, there was an increase in the circulating concentration of several adipokines, including plasma leptin, which was associated with inactivity-induced increases in plasma insulin and absent from adipose tissue cultured ex vivo under standardized culture conditions.

Physical inactivity–induced disturbances to adipokine concentrations such as leptin, without changes to fat mass, could have profound metabolic implications outside a clinical facility when energy intake is not tightly controlled.
Original languageEnglish
Pages (from-to)177-191
Number of pages15
JournalThe Journal of Clinical Endocrinology & Metabolism
Issue number1
Early online date4 Sept 2021
Publication statusPublished - 1 Jan 2022

Bibliographical note

Funding: Financial support was provided by the Biotechnology
and Biological Sciences Research Council Biotechnology and
Biological Sciences Research Council grant BB/N004809/1

Data Availability: Supplementary data cited in this work
can be accessed at Figshare (23) (https://doi.org/10.6084/
m9.figshare.15138231). Sequencing data sets associated with this
work are available from GenBank (GSE1822303). All remaining
datasets generated during the current study are available in the
University of Bath Data Repository (69) (https://doi.org/10.15125/


  • Adipose tissue
  • immunometabolism
  • physical inactivity

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical


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