The Arabidopsis TIR-NBS-LRR protein CSA1 guards BAK1-BIR3 homeostasis and mediates convergence of pattern- and effector-induced immune responses

Sarina Schulze, Liping Yu, Chenlei Hua, Lisha Zhang, Dagmar Kolb, Hannah Weber, Alexandra Ehinger, Svenja C. Saile, Mark Stahl, Mirita Franz-Wachtel, Lei Li, Farid El Kasmi, Thorsten Nürnberger, Volkan Cevik, Birgit Kemmerling

Research output: Contribution to journalArticlepeer-review

Abstract

Arabidopsis BAK1/SERK3, a co-receptor of leucine-rich repeat pattern recognition receptors (PRRs), mediates pattern-triggered immunity (PTI). Genetic inactivation of BAK1 or BAK1-interacting receptor-like kinases (BIRs) causes cell death, but the direct mechanisms leading to such deregulation remains unclear. Here, we found that the TIR-NBS-LRR protein CONSTITUTIVE SHADE AVOIDANCE 1 (CSA1) physically interacts with BIR3, but not with BAK1. CSA1 mediates cell death in bak1-4 and bak1-4 bir3-2 mutants via components of effector-triggered immunity-(ETI) pathways. Effector HopB1-mediated perturbation of BAK1 also results in CSA1-dependent cell death. Likewise, microbial pattern pg23-induced cell death, but not PTI responses, requires CSA1. Thus, we show that CSA1 guards BIR3 BAK1 homeostasis and integrates pattern- and effector-mediated cell death pathways downstream of BAK1. De-repression of CSA1 in the absence of intact BAK1 and BIR3 triggers ETI cell death. This suggests that PTI and ETI pathways are activated downstream of BAK1 for efficient plant immunity.

Original languageEnglish
Pages (from-to)1717-1731.e6
JournalCell Host and Microbe
Volume30
Issue number12
Early online date28 Nov 2022
DOIs
Publication statusPublished - 14 Dec 2022

Keywords

  • Arabidopsis
  • BAK1
  • BIR3
  • cell death
  • CHS3
  • CSA1
  • guard
  • NLR
  • PTI-ETI cross talk
  • receptor kinase

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Virology

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