The emergence of plasmid-borne cfr-mediated linezolid resistant-staphylococci in Vietnam

Le Thuy Thi Nguyen, Khanh Ngau Thi Nguyen, Phuc Nhi Thi Anh Le, Fabio Cafini, Ben Pascoe, Samuel K. Sheppard, Thanh Bao Nguyen, Thien Phuc Nguyen Hoang, Thuy Vy Nguyen, Tram T.k. Pham, Kazuya Morikawa, Dang Quan Nguyen, Hoa Xo Duong

Research output: Contribution to journalArticlepeer-review

5 Citations (SciVal)


Objectives: Linezolid is one of the last resort antibiotics effectively used in the treatment of infections caused by multidrug-resistant Gram-positive bacteria. Recent outbreaks of Linezolid resistance have been the great concern worldwide, while many countries have not experienced it. In this work, we aimed to evaluate the existence of linezolid resistance and further clarify potential resistance mechanism(s) in staphylococcal isolates obtained from the hospital in Vietnam, a country in which linezolid resistance had not been previously detected. Methods: Seventy staphylococcal clinical isolates including MRSA (n = 63) and methicillin-resistant coagulase-negative staphylococci (MRCNS, n = 7) were collected and analyzed for linezolid resistance. Linezolid-resistant isolates were submitted for whole genome sequencing to search for the resistance determinants. Results: We identified two coagulase-negative staphylococcal isolates that were resistant to linezolid. Whole genome sequencing revealed several alterations in the 23S rRNA and L3, L17, L22, L24, L30 ribosomal proteins. Importantly, both isolates harbour the chloramphenicol/florfenicol resistance (cfr) gene on a plasmid. The plasmid was closely identical to the pLRSA417 plasmid that was originally reported in China. Conclusions: To the best of our knowledge, this is the first report of cfr-mediated linezolid resistance in clinically isolated staphylococci in Vietnam. We suggest that adequate surveillance is necessary to monitor the dissemination of linezolid resistance among staphylococcal species and other important pathogens.

Original languageEnglish
Pages (from-to)462-465
Number of pages4
JournalJournal of Global Antimicrobial Resistance
Early online date27 Apr 2020
Publication statusPublished - 30 Sept 2020


  • Coagulase-negative staphylococci
  • Linezolid resistance
  • Vietnam
  • cfr

ASJC Scopus subject areas

  • Microbiology
  • Immunology and Allergy
  • Immunology
  • Microbiology (medical)


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