Abstract
The effects of two fusion inhibitors on the lipid polymorphism of N-methylated dioleoylphosphatidylethanolamine were studied using temperature-resolved, small-angle X-ray diffraction. The inhibitory role of the tri-peptide carbobenzoxy-D-phenylalanine-L-phenylalanine-glycine and the lipid 1-lauroyl-2-hydroxy-sn-glycero-3-phosphocholine in the fusion pathway was studied, using the non-lamellar phase behaviour of the lipid as a model. We used p15EK, the N-terminal region of gp41 from feline leukaemia virus as promoter of membrane fusion, and measured the structural parameters of each observed lipid phase as a function of temperature. The fusion inhibitors were found to impede the expression of negative curvature of lipid monolayers even in the presence of fusion peptide. The results of this study are interpreted in relation to models of the membrane fusion mechanism.
| Original language | English |
|---|---|
| Pages (from-to) | 119-128 |
| Number of pages | 10 |
| Journal | Biochimica et Biophysica Acta - Biomembranes |
| Volume | 1561 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 19 Mar 2002 |
Keywords
- Biomembrane fusion
- Cubic phase
- Hexagonal phase
- Inhibitor
- Lamellar phase
- Small angle X-ray diffraction
- Small angle X-ray scattering
- Time resolved X-ray diffraction
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Cell Biology
Cite this
The effect of fusion inhibitors on the phase behaviour of N-methylated dioleoylphosphatidylethanolamine. / Darkes, Malcolm J.M.; Harroun, Thad A.; Davies, Sarah M.A.; Bradshaw, Jeremy P.
In: Biochimica et Biophysica Acta - Biomembranes, Vol. 1561, No. 1, 19.03.2002, p. 119-128.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The effect of fusion inhibitors on the phase behaviour of N-methylated dioleoylphosphatidylethanolamine
AU - Darkes, Malcolm J.M.
AU - Harroun, Thad A.
AU - Davies, Sarah M.A.
AU - Bradshaw, Jeremy P.
PY - 2002/3/19
Y1 - 2002/3/19
N2 - The effects of two fusion inhibitors on the lipid polymorphism of N-methylated dioleoylphosphatidylethanolamine were studied using temperature-resolved, small-angle X-ray diffraction. The inhibitory role of the tri-peptide carbobenzoxy-D-phenylalanine-L-phenylalanine-glycine and the lipid 1-lauroyl-2-hydroxy-sn-glycero-3-phosphocholine in the fusion pathway was studied, using the non-lamellar phase behaviour of the lipid as a model. We used p15EK, the N-terminal region of gp41 from feline leukaemia virus as promoter of membrane fusion, and measured the structural parameters of each observed lipid phase as a function of temperature. The fusion inhibitors were found to impede the expression of negative curvature of lipid monolayers even in the presence of fusion peptide. The results of this study are interpreted in relation to models of the membrane fusion mechanism.
AB - The effects of two fusion inhibitors on the lipid polymorphism of N-methylated dioleoylphosphatidylethanolamine were studied using temperature-resolved, small-angle X-ray diffraction. The inhibitory role of the tri-peptide carbobenzoxy-D-phenylalanine-L-phenylalanine-glycine and the lipid 1-lauroyl-2-hydroxy-sn-glycero-3-phosphocholine in the fusion pathway was studied, using the non-lamellar phase behaviour of the lipid as a model. We used p15EK, the N-terminal region of gp41 from feline leukaemia virus as promoter of membrane fusion, and measured the structural parameters of each observed lipid phase as a function of temperature. The fusion inhibitors were found to impede the expression of negative curvature of lipid monolayers even in the presence of fusion peptide. The results of this study are interpreted in relation to models of the membrane fusion mechanism.
KW - Biomembrane fusion
KW - Cubic phase
KW - Hexagonal phase
KW - Inhibitor
KW - Lamellar phase
KW - Small angle X-ray diffraction
KW - Small angle X-ray scattering
KW - Time resolved X-ray diffraction
UR - http://www.scopus.com/inward/record.url?scp=0037133723&partnerID=8YFLogxK
U2 - 10.1016/S0005-2736(01)00464-3
DO - 10.1016/S0005-2736(01)00464-3
M3 - Article
VL - 1561
SP - 119
EP - 128
JO - Biochimica Et Biophysica Acta-Biomembranes
JF - Biochimica Et Biophysica Acta-Biomembranes
SN - 0005-2736
IS - 1
ER -