The effect of fusion inhibitors on the phase behaviour of N-methylated dioleoylphosphatidylethanolamine

Malcolm J.M. Darkes; Thad A. Harroun; Sarah M.A. Davies; Jeremy P. Bradshaw

doi:10.1016/S0005-2736(01)00464-3

The effect of fusion inhibitors on the phase behaviour of N-methylated dioleoylphosphatidylethanolamine

Malcolm J.M. Darkes, Thad A. Harroun, Sarah M.A. Davies, Jeremy P. Bradshaw

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The effects of two fusion inhibitors on the lipid polymorphism of N-methylated dioleoylphosphatidylethanolamine were studied using temperature-resolved, small-angle X-ray diffraction. The inhibitory role of the tri-peptide carbobenzoxy-D-phenylalanine-L-phenylalanine-glycine and the lipid 1-lauroyl-2-hydroxy-sn-glycero-3-phosphocholine in the fusion pathway was studied, using the non-lamellar phase behaviour of the lipid as a model. We used p15EK, the N-terminal region of gp41 from feline leukaemia virus as promoter of membrane fusion, and measured the structural parameters of each observed lipid phase as a function of temperature. The fusion inhibitors were found to impede the expression of negative curvature of lipid monolayers even in the presence of fusion peptide. The results of this study are interpreted in relation to models of the membrane fusion mechanism.

Original language	English
Pages (from-to)	119-128
Number of pages	10
Journal	Biochimica et Biophysica Acta - Biomembranes
Volume	1561
Issue number	1
DOIs	https://doi.org/10.1016/S0005-2736(01)00464-3
Publication status	Published - 19 Mar 2002

Keywords

Biomembrane fusion
Cubic phase
Hexagonal phase
Inhibitor
Lamellar phase
Small angle X-ray diffraction
Small angle X-ray scattering
Time resolved X-ray diffraction

ASJC Scopus subject areas

Biophysics
Biochemistry
Cell Biology

Access to Document

10.1016/S0005-2736(01)00464-3

Link to publication in Scopus

Fingerprint Dive into the research topics of 'The effect of fusion inhibitors on the phase behaviour of N-methylated dioleoylphosphatidylethanolamine'. Together they form a unique fingerprint.

Cite this

@article{a5288302cfca4687b276c9e2ad856844,

title = "The effect of fusion inhibitors on the phase behaviour of N-methylated dioleoylphosphatidylethanolamine",

abstract = "The effects of two fusion inhibitors on the lipid polymorphism of N-methylated dioleoylphosphatidylethanolamine were studied using temperature-resolved, small-angle X-ray diffraction. The inhibitory role of the tri-peptide carbobenzoxy-D-phenylalanine-L-phenylalanine-glycine and the lipid 1-lauroyl-2-hydroxy-sn-glycero-3-phosphocholine in the fusion pathway was studied, using the non-lamellar phase behaviour of the lipid as a model. We used p15EK, the N-terminal region of gp41 from feline leukaemia virus as promoter of membrane fusion, and measured the structural parameters of each observed lipid phase as a function of temperature. The fusion inhibitors were found to impede the expression of negative curvature of lipid monolayers even in the presence of fusion peptide. The results of this study are interpreted in relation to models of the membrane fusion mechanism.",

keywords = "Biomembrane fusion, Cubic phase, Hexagonal phase, Inhibitor, Lamellar phase, Small angle X-ray diffraction, Small angle X-ray scattering, Time resolved X-ray diffraction",

author = "Darkes, {Malcolm J.M.} and Harroun, {Thad A.} and Davies, {Sarah M.A.} and Bradshaw, {Jeremy P.}",

year = "2002",

month = mar,

day = "19",

doi = "10.1016/S0005-2736(01)00464-3",

language = "English",

volume = "1561",

pages = "119--128",

journal = "Biochimica Et Biophysica Acta-Biomembranes",

issn = "0005-2736",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - The effect of fusion inhibitors on the phase behaviour of N-methylated dioleoylphosphatidylethanolamine

AU - Darkes, Malcolm J.M.

AU - Harroun, Thad A.

AU - Davies, Sarah M.A.

AU - Bradshaw, Jeremy P.

PY - 2002/3/19

Y1 - 2002/3/19

N2 - The effects of two fusion inhibitors on the lipid polymorphism of N-methylated dioleoylphosphatidylethanolamine were studied using temperature-resolved, small-angle X-ray diffraction. The inhibitory role of the tri-peptide carbobenzoxy-D-phenylalanine-L-phenylalanine-glycine and the lipid 1-lauroyl-2-hydroxy-sn-glycero-3-phosphocholine in the fusion pathway was studied, using the non-lamellar phase behaviour of the lipid as a model. We used p15EK, the N-terminal region of gp41 from feline leukaemia virus as promoter of membrane fusion, and measured the structural parameters of each observed lipid phase as a function of temperature. The fusion inhibitors were found to impede the expression of negative curvature of lipid monolayers even in the presence of fusion peptide. The results of this study are interpreted in relation to models of the membrane fusion mechanism.

AB - The effects of two fusion inhibitors on the lipid polymorphism of N-methylated dioleoylphosphatidylethanolamine were studied using temperature-resolved, small-angle X-ray diffraction. The inhibitory role of the tri-peptide carbobenzoxy-D-phenylalanine-L-phenylalanine-glycine and the lipid 1-lauroyl-2-hydroxy-sn-glycero-3-phosphocholine in the fusion pathway was studied, using the non-lamellar phase behaviour of the lipid as a model. We used p15EK, the N-terminal region of gp41 from feline leukaemia virus as promoter of membrane fusion, and measured the structural parameters of each observed lipid phase as a function of temperature. The fusion inhibitors were found to impede the expression of negative curvature of lipid monolayers even in the presence of fusion peptide. The results of this study are interpreted in relation to models of the membrane fusion mechanism.

KW - Biomembrane fusion

KW - Cubic phase

KW - Hexagonal phase

KW - Inhibitor

KW - Lamellar phase

KW - Small angle X-ray diffraction

KW - Small angle X-ray scattering

KW - Time resolved X-ray diffraction

UR - http://www.scopus.com/inward/record.url?scp=0037133723&partnerID=8YFLogxK

U2 - 10.1016/S0005-2736(01)00464-3

DO - 10.1016/S0005-2736(01)00464-3

M3 - Article

C2 - 11988186

AN - SCOPUS:0037133723

VL - 1561

SP - 119

EP - 128

JO - Biochimica Et Biophysica Acta-Biomembranes

JF - Biochimica Et Biophysica Acta-Biomembranes

SN - 0005-2736

IS - 1

ER -