The effect of fusion inhibitors on the phase behaviour of N-methylated dioleoylphosphatidylethanolamine

Malcolm J.M. Darkes, Thad A. Harroun, Sarah M.A. Davies, Jeremy P. Bradshaw

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The effects of two fusion inhibitors on the lipid polymorphism of N-methylated dioleoylphosphatidylethanolamine were studied using temperature-resolved, small-angle X-ray diffraction. The inhibitory role of the tri-peptide carbobenzoxy-D-phenylalanine-L-phenylalanine-glycine and the lipid 1-lauroyl-2-hydroxy-sn-glycero-3-phosphocholine in the fusion pathway was studied, using the non-lamellar phase behaviour of the lipid as a model. We used p15EK, the N-terminal region of gp41 from feline leukaemia virus as promoter of membrane fusion, and measured the structural parameters of each observed lipid phase as a function of temperature. The fusion inhibitors were found to impede the expression of negative curvature of lipid monolayers even in the presence of fusion peptide. The results of this study are interpreted in relation to models of the membrane fusion mechanism.

Original languageEnglish
Pages (from-to)119-128
Number of pages10
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1561
Issue number1
DOIs
Publication statusPublished - 19 Mar 2002

Keywords

  • Biomembrane fusion
  • Cubic phase
  • Hexagonal phase
  • Inhibitor
  • Lamellar phase
  • Small angle X-ray diffraction
  • Small angle X-ray scattering
  • Time resolved X-ray diffraction

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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