The Effect of an Acute Bout of Exercise on Circulating Vitamin D Metabolite Concentrations: A Randomised Crossover Study in Healthy Adults

Sophie E. Davies; Oliver J. Perkin; James A. Betts; Javier T. Gonzalez; Martin Hewison; Carl Jenkinson; Kerry S. Jones; Sarah R. Meadows; Damon A. Parkington; Albert Koulman; Dylan Thompson

doi:10.1113/JP286395

The Effect of an Acute Bout of Exercise on Circulating Vitamin D Metabolite Concentrations: A Randomised Crossover Study in Healthy Adults

Sophie E. Davies, Oliver J. Perkin, James A. Betts, Javier T. Gonzalez, Martin Hewison, Carl Jenkinson, Kerry S. Jones, Sarah R. Meadows, Damon A. Parkington, Albert Koulman, Dylan Thompson

Research output: Contribution to journal › Article › peer-review

Abstract

Abstract: The effect of acute exercise on circulating concentrations of vitamin D metabolites is unclear. To address this knowledge gap, we examined the effect of a bout of treadmill-based exercise versus rest on circulating concentrations of 25(OH)D ₃, 25(OH)D ₂, 3-epi-25(OH)D ₃, 24,25(OH) ₂D ₃, 1,25(OH) ₂D ₃, and vitamin D ₂ and D ₃ in healthy men and women. Thirty-three healthy adults (14 females, 41 (15) years, body mass index 26.2 (3.7) kg/m ², (Formula presented.) 36.2 (9.2) ml/kg/min; mean (SD)) completed two laboratory visits involving 60 min of moderate-intensity treadmill exercise (60% (Formula presented.)) versus 60 min of seated rest, both in an overnight fasted-state, as part of a randomised crossover design. Venous blood samples were drawn at baseline, immediately (0 h), 1 h and 24 h after the exercise or rest-period. There was a significant time × trial interaction effect for total circulating 25(OH)D (P = 0.0148), 25(OH)D ₃ (P = 0.0127) and 1,25(OH) ₂D ₃ (P = 0.0226). Immediately post-exercise, 25(OH)D, 25(OH)D ₃ and 1,25(OH) ₂D ₃ concentrations were significantly elevated compared to the control resting condition, and 1,25(OH) ₂D ₃ remained significantly elevated 1 h later. Circulating albumin, vitamin D binding protein, calcium and parathyroid hormone were elevated immediately post-exercise. Thus, an acute bout of moderate intensity exercise transiently increases concentrations of circulating 25(OH)D and 1,25(OH) ₂D ₃ compared to resting conditions. (Figure presented.). Key points: Observational studies suggest that acute exercise might change circulating concentrations of vitamin D metabolites, but this has not been investigated using randomised crossover studies and using robust analytical procedures. In this study, we used a randomised crossover design to examine the effect of a bout of treadmill-based exercise (vs. rest) on circulating concentrations of a wide range of vitamin D metabolites in healthy humans. We show that an acute bout of moderate intensity exercise transiently increases concentrations of circulating 25(OH)D and 1,25(OH) ₂D ₃ compared to resting conditions. These findings indicate that regular exercise could lead to transient but regular windows of enhanced vitamin D biological action.

Original language	English
Journal	The Journal of Physiology
Volume	602
Issue number	17
Early online date	4 Aug 2024
DOIs	https://doi.org/10.1113/JP286395
Publication status	Published - 1 Sept 2024

Bibliographical note

For the purpose of open access, the author has applied a creative commons attribution (CC BY) licence to any author accepted manuscript version arising.

Data Availability Statement

The data is available at: https://doi.org/10.15125/BATH-01406.For the purpose of open access, the author has applied a creative commons attribution (CC BY) licence to any author accepted manuscript version arising.

Acknowledgements

The authors thank the participants for their time and commitment to this study. The authors also thank Matt Talbot and Lili Parker for their help on trial days.
The authors would also like to thank Ben Nash and colleagues at the Department of Clinical Biochemistry, University Hospitals Bristol and Weston NHS Foundation Trust for the biochemical analysis of plasma PTH; Russell Davies at the University of Bath for his technical support with biochemical analysis of samples.
S.E.D thanks Funds for Women Graduates for providing financial support towards living costs which has allowed the completion of this work.
This research was supported by the NIHR Cambridge Biomedical Research Centre (NIHR203312). The views expressed are those of the authors and not necessarily those of the NIHR or the Department of Health and Social Care.

Funding

The authors thank the participants for their time and commitment to this study. The authors also thank Matt Talbot and Lili Parker for their help on trial days. The authors would also like to thank Ben Nash and colleagues at the Department of Clinical Biochemistry, University Hospitals Bristol and Weston NHS Foundation Trust for the biochemical analysis of plasma PTH; Russell Davies at the University of Bath for his technical support with biochemical analysis of samples. SED thanks Funds for Women Graduates for providing financial support towards living costs which has allowed the completion of this work. This research was supported by the NIHR Cambridge Biomedical Research Centre (NIHR203312). The views expressed are those of the authors and not necessarily those of the NIHR or the Department of Health and Social Care. This work was funded by a grant from Biotechnology and Biosciences Research Council (BBSRC) (BB/R018928/1).

Funders	Funder number
Funds for Women Graduates
Biotechnology and Biosciences Research Council	BB/R018928/1
NIHR Cambridge Biomedical Research Centre	NIHR203312
NIHR Cambridge Biomedical Research Centre

Keywords

1,25(OH) D
25(OH)D
calcitriol
lipolysis
metabolism
physiology

ASJC Scopus subject areas

Physiology

Access to Document

10.1113/JP286395Licence: CC BY

Cite this

Davies, S. E., Perkin, O. J., Betts, J. A., Gonzalez, J. T., Hewison, M., Jenkinson, C., Jones, K. S., Meadows, S. R., Parkington, D. A., Koulman, A., & Thompson, D. (2024). The Effect of an Acute Bout of Exercise on Circulating Vitamin D Metabolite Concentrations: A Randomised Crossover Study in Healthy Adults. The Journal of Physiology, 602(17). https://doi.org/10.1113/JP286395

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title = "The Effect of an Acute Bout of Exercise on Circulating Vitamin D Metabolite Concentrations: A Randomised Crossover Study in Healthy Adults",

abstract = "Abstract: The effect of acute exercise on circulating concentrations of vitamin D metabolites is unclear. To address this knowledge gap, we examined the effect of a bout of treadmill-based exercise versus rest on circulating concentrations of 25(OH)D 3, 25(OH)D 2, 3-epi-25(OH)D 3, 24,25(OH) 2D 3, 1,25(OH) 2D 3, and vitamin D 2 and D 3 in healthy men and women. Thirty-three healthy adults (14 females, 41 (15) years, body mass index 26.2 (3.7) kg/m 2, (Formula presented.) 36.2 (9.2) ml/kg/min; mean (SD)) completed two laboratory visits involving 60 min of moderate-intensity treadmill exercise (60% (Formula presented.)) versus 60 min of seated rest, both in an overnight fasted-state, as part of a randomised crossover design. Venous blood samples were drawn at baseline, immediately (0 h), 1 h and 24 h after the exercise or rest-period. There was a significant time × trial interaction effect for total circulating 25(OH)D (P = 0.0148), 25(OH)D 3 (P = 0.0127) and 1,25(OH) 2D 3 (P = 0.0226). Immediately post-exercise, 25(OH)D, 25(OH)D 3 and 1,25(OH) 2D 3 concentrations were significantly elevated compared to the control resting condition, and 1,25(OH) 2D 3 remained significantly elevated 1 h later. Circulating albumin, vitamin D binding protein, calcium and parathyroid hormone were elevated immediately post-exercise. Thus, an acute bout of moderate intensity exercise transiently increases concentrations of circulating 25(OH)D and 1,25(OH) 2D 3 compared to resting conditions. (Figure presented.). Key points: Observational studies suggest that acute exercise might change circulating concentrations of vitamin D metabolites, but this has not been investigated using randomised crossover studies and using robust analytical procedures. In this study, we used a randomised crossover design to examine the effect of a bout of treadmill-based exercise (vs. rest) on circulating concentrations of a wide range of vitamin D metabolites in healthy humans. We show that an acute bout of moderate intensity exercise transiently increases concentrations of circulating 25(OH)D and 1,25(OH) 2D 3 compared to resting conditions. These findings indicate that regular exercise could lead to transient but regular windows of enhanced vitamin D biological action.",

keywords = "1,25(OH) D, 25(OH)D, calcitriol, lipolysis, metabolism, physiology",

author = "Davies, {Sophie E.} and Perkin, {Oliver J.} and Betts, {James A.} and Gonzalez, {Javier T.} and Martin Hewison and Carl Jenkinson and Jones, {Kerry S.} and Meadows, {Sarah R.} and Parkington, {Damon A.} and Albert Koulman and Dylan Thompson",

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T2 - A Randomised Crossover Study in Healthy Adults

AU - Davies, Sophie E.

AU - Perkin, Oliver J.

AU - Betts, James A.

AU - Gonzalez, Javier T.

AU - Hewison, Martin

AU - Jenkinson, Carl

AU - Jones, Kerry S.

AU - Meadows, Sarah R.

AU - Parkington, Damon A.

AU - Koulman, Albert

AU - Thompson, Dylan

N1 - For the purpose of open access, the author has applied a creative commons attribution (CC BY) licence to any author accepted manuscript version arising.

PY - 2024/9/1

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N2 - Abstract: The effect of acute exercise on circulating concentrations of vitamin D metabolites is unclear. To address this knowledge gap, we examined the effect of a bout of treadmill-based exercise versus rest on circulating concentrations of 25(OH)D 3, 25(OH)D 2, 3-epi-25(OH)D 3, 24,25(OH) 2D 3, 1,25(OH) 2D 3, and vitamin D 2 and D 3 in healthy men and women. Thirty-three healthy adults (14 females, 41 (15) years, body mass index 26.2 (3.7) kg/m 2, (Formula presented.) 36.2 (9.2) ml/kg/min; mean (SD)) completed two laboratory visits involving 60 min of moderate-intensity treadmill exercise (60% (Formula presented.)) versus 60 min of seated rest, both in an overnight fasted-state, as part of a randomised crossover design. Venous blood samples were drawn at baseline, immediately (0 h), 1 h and 24 h after the exercise or rest-period. There was a significant time × trial interaction effect for total circulating 25(OH)D (P = 0.0148), 25(OH)D 3 (P = 0.0127) and 1,25(OH) 2D 3 (P = 0.0226). Immediately post-exercise, 25(OH)D, 25(OH)D 3 and 1,25(OH) 2D 3 concentrations were significantly elevated compared to the control resting condition, and 1,25(OH) 2D 3 remained significantly elevated 1 h later. Circulating albumin, vitamin D binding protein, calcium and parathyroid hormone were elevated immediately post-exercise. Thus, an acute bout of moderate intensity exercise transiently increases concentrations of circulating 25(OH)D and 1,25(OH) 2D 3 compared to resting conditions. (Figure presented.). Key points: Observational studies suggest that acute exercise might change circulating concentrations of vitamin D metabolites, but this has not been investigated using randomised crossover studies and using robust analytical procedures. In this study, we used a randomised crossover design to examine the effect of a bout of treadmill-based exercise (vs. rest) on circulating concentrations of a wide range of vitamin D metabolites in healthy humans. We show that an acute bout of moderate intensity exercise transiently increases concentrations of circulating 25(OH)D and 1,25(OH) 2D 3 compared to resting conditions. These findings indicate that regular exercise could lead to transient but regular windows of enhanced vitamin D biological action.

AB - Abstract: The effect of acute exercise on circulating concentrations of vitamin D metabolites is unclear. To address this knowledge gap, we examined the effect of a bout of treadmill-based exercise versus rest on circulating concentrations of 25(OH)D 3, 25(OH)D 2, 3-epi-25(OH)D 3, 24,25(OH) 2D 3, 1,25(OH) 2D 3, and vitamin D 2 and D 3 in healthy men and women. Thirty-three healthy adults (14 females, 41 (15) years, body mass index 26.2 (3.7) kg/m 2, (Formula presented.) 36.2 (9.2) ml/kg/min; mean (SD)) completed two laboratory visits involving 60 min of moderate-intensity treadmill exercise (60% (Formula presented.)) versus 60 min of seated rest, both in an overnight fasted-state, as part of a randomised crossover design. Venous blood samples were drawn at baseline, immediately (0 h), 1 h and 24 h after the exercise or rest-period. There was a significant time × trial interaction effect for total circulating 25(OH)D (P = 0.0148), 25(OH)D 3 (P = 0.0127) and 1,25(OH) 2D 3 (P = 0.0226). Immediately post-exercise, 25(OH)D, 25(OH)D 3 and 1,25(OH) 2D 3 concentrations were significantly elevated compared to the control resting condition, and 1,25(OH) 2D 3 remained significantly elevated 1 h later. Circulating albumin, vitamin D binding protein, calcium and parathyroid hormone were elevated immediately post-exercise. Thus, an acute bout of moderate intensity exercise transiently increases concentrations of circulating 25(OH)D and 1,25(OH) 2D 3 compared to resting conditions. (Figure presented.). Key points: Observational studies suggest that acute exercise might change circulating concentrations of vitamin D metabolites, but this has not been investigated using randomised crossover studies and using robust analytical procedures. In this study, we used a randomised crossover design to examine the effect of a bout of treadmill-based exercise (vs. rest) on circulating concentrations of a wide range of vitamin D metabolites in healthy humans. We show that an acute bout of moderate intensity exercise transiently increases concentrations of circulating 25(OH)D and 1,25(OH) 2D 3 compared to resting conditions. These findings indicate that regular exercise could lead to transient but regular windows of enhanced vitamin D biological action.

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The Effect of an Acute Bout of Exercise on Circulating Vitamin D Metabolite Concentrations: A Randomised Crossover Study in Healthy Adults

Abstract

Bibliographical note

Data Availability Statement

Acknowledgements

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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Fingerprint

Mobilising Vitamin D Sequestered In Adipose Tissue In Humans

Dataset for "The Effect of an Acute Bout of Exercise on Circulating Vitamin D Metabolite Concentrations: A Randomised Crossover Study in Healthy Adults"

Cite this

The Effect of an Acute Bout of Exercise on Circulating Vitamin D Metabolite Concentrations: A Randomised Crossover Study in Healthy Adults

Abstract

Bibliographical note

Data Availability Statement

Acknowledgements

Funding

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Projects

Mobilising Vitamin D Sequestered In Adipose Tissue In Humans

Datasets

Dataset for "The Effect of an Acute Bout of Exercise on Circulating Vitamin D Metabolite Concentrations: A Randomised Crossover Study in Healthy Adults"

Cite this