Previous studies have revealed that attention and inhibition are impaired in individuals with elevated symptoms of depression and anxiety. Virtual reality (VR)-based neuropsychological assessment may be a valid instrument for assessing attention and inhibition given its higher ecological validity when compared to classical tests. However, it is still unclear as to whether a VR assessment can predict depression and anxiety with the same or higher level of efectiveness and adherence as classical neuropsychological measures. The current study examined the efectiveness of a new VR test, Nesplora Aquarium, by testing participants with low (N=41) and elevated (N=41) symptoms of depression and anxiety. Participants completed a continuous performance test where they had to respond to stimuli (species of fsh) in a virtual aquarium, as well as paper-and-pencil and computerised tests. Participants’ performance in Nesplora Aquarium was positively associated with classic measures of attention and inhibition, and efectively predicted symptoms of depression and anxiety above and beyond traditional cognitive measures such as psychomotor speed and executive functioning, spatial working memory span. Hence, VR is a safe, enjoyable, efective and more ecological alternative for the assessment of attention and inhibition among individuals with elevated anxiety and depression symptoms.