The diversity and geographical structure of orientia tsutsugamushi strains from scrub typhus patients in Laos

Rattanaphone Phetsouvanh, Piengchan Sonthayanon, Sasithon Pukrittayakamee, Daniel H. Paris, Paul N. Newton, Edward J. Feil, Nicholas P.J. Day

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Orientia tsutsugamushi is the causative agent of scrub typhus, a disease transmitted by Leptotrombidium mites which is responsible for a severe and under-reported public health burden throughout Southeast Asia. Here we use multilocus sequence typing (MLST) to characterize 74 clinical isolates from three geographic locations in the Lao PDR (Laos), and compare them with isolates described from Udon Thani, northeast Thailand. The data confirm high levels of diversity and recombination within the natural O. tsutsugamushi population, and a rate of mixed infection of ~8%. We compared the relationships and geographical structuring of the strains and populations using allele based approaches (eBURST), phylogenetic approaches, and by calculating F-statistics (FST). These analyses all point towards low levels of population differentiation between isolates from Vientiane and Udon Thani, cities which straddle the Mekong River which defines the Lao/Thai border, but with a very distinct population in Salavan, southern Laos. These data highlight how land use, as well as the movement of hosts and vectors, may impact on the epidemiology of zoonotic infections.

Original languageEnglish
Article numberA008
JournalPLoS Neglected Tropical Diseases
Volume9
Issue number8
DOIs
Publication statusPublished - 28 Aug 2015

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this