The development of a novel AND logic based fluorescence probe for the detection of peroxynitrite and GSH

Adam C. Sedgwick, Hai Hao Han, Jordan E. Gardiner, Steven D. Bull, Xiao-Peng He, Tony D. James

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

We have developed a novel AND logic based fluorescence probe for the simultaneous detection of ONOO− and GSH (GSH-PF3). The GSH-PF3 probe was synthesised over three steps starting from commercially available fluorescein. The probe was constructed by attaching the GSH reactive motif, 2,4-dinitrobenzenesulfonyl, to the previously reported boronate fluorescence based probe, PF3. GSH-PF3 produced only a small fluorescence response towards the addition of GSH or ONOO− separately. However, when the probe was exposed to both analytes, there was a significant (40-fold) fluorescence enhancement. GSH-PF3 demonstrated an excellent selectivity towards both GSH and ONOO−. In cellular imaging experiments the probe was shown to be cell permeable with no ‘turn-on’ response observed for the addition of either GSH or ONOO− separately. However, in the presence of both analytes, a clear fluorescence response was observed in live cells. GSH-PF3 was further able to monitor the co-existence of metabolically produced ONOO− and GSH by exogenous stimulation.
Original languageEnglish
Pages (from-to)3672-3676
Number of pages5
JournalChemical Science
Volume9
Issue number15
Early online date16 Mar 2018
DOIs
Publication statusPublished - 21 Apr 2018

ASJC Scopus subject areas

  • Chemistry(all)

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