The chicken talpid3 gene encodes a novel protein essential for Hedgehog signaling

Megan Davey, Robert Paton, Yili Yin, Maike Schmidt, Fiona Bangs, David Morrice, Smith Terence , Paul Buxton, Despina Stamataki, Mikiko Tanaka, Andrea Münsterberg, James Briscoe, Cheryll Tickle, Dave Burt

Research output: Contribution to journalArticle

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Abstract

Talpid3 is a classical chicken mutant with abnormal limb patterning and malformations in other regions of the embryo known to depend on Hedgehog signaling. We combined the ease of manipulating chicken embryos with emerging knowledge of the chicken genome to reveal directly the basis of defective Hedgehog signal transduction in talpid3 embryos and to identify the talpid3 gene. We show in several regions of the embryo that the talpid3 phenotype is completely ligand independent and demonstrate for the first time that talpid3 is absolutely required for the function of both Gli repressor and activator in the intracellular Hedgehog pathway. We map the talpid3 locus to chromosome 5 and find a frameshift mutation in a KIAA0586 ortholog (ENSGALG00000012025), a gene not previously attributed with any known function. We show a direct causal link between KIAA0586 and the mutant phenotype by rescue experiments. KIAA0586 encodes a novel protein, apparently specific to vertebrates, that localizes to the cytoplasm. We show that Gli3 processing is abnormal in talpid3 mutant cells but that Gli3 can still translocate to the nucleus. These results suggest that the talpid3 protein operates in the cytoplasm to regulate the activity of both Gli repressor and activator proteins.
Original languageEnglish
Pages (from-to)1365-1377
JournalGenes & Development
Volume20
DOIs
Publication statusPublished - 2006

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Hedgehog Proteins
Hedgehogs
Chickens
Embryonic Structures
Genes
Cytoplasm
Repressor Proteins
Phenotype
Chromosomes, Human, Pair 5
Frameshift Mutation
Vertebrates
Signal Transduction
Proteins
Extremities
Genome
Ligands

Cite this

Davey, M., Paton, R., Yin, Y., Schmidt, M., Bangs, F., Morrice, D., ... Burt, D. (2006). The chicken talpid3 gene encodes a novel protein essential for Hedgehog signaling. Genes & Development, 20, 1365-1377. https://doi.org/10.1101/gad.369106

The chicken talpid3 gene encodes a novel protein essential for Hedgehog signaling. / Davey, Megan; Paton, Robert; Yin, Yili; Schmidt, Maike ; Bangs, Fiona; Morrice, David ; Terence , Smith; Buxton, Paul ; Stamataki, Despina ; Tanaka, Mikiko ; Münsterberg, Andrea ; Briscoe, James; Tickle, Cheryll; Burt, Dave .

In: Genes & Development, Vol. 20, 2006, p. 1365-1377.

Research output: Contribution to journalArticle

Davey, M, Paton, R, Yin, Y, Schmidt, M, Bangs, F, Morrice, D, Terence , S, Buxton, P, Stamataki, D, Tanaka, M, Münsterberg, A, Briscoe, J, Tickle, C & Burt, D 2006, 'The chicken talpid3 gene encodes a novel protein essential for Hedgehog signaling', Genes & Development, vol. 20, pp. 1365-1377. https://doi.org/10.1101/gad.369106
Davey, Megan ; Paton, Robert ; Yin, Yili ; Schmidt, Maike ; Bangs, Fiona ; Morrice, David ; Terence , Smith ; Buxton, Paul ; Stamataki, Despina ; Tanaka, Mikiko ; Münsterberg, Andrea ; Briscoe, James ; Tickle, Cheryll ; Burt, Dave . / The chicken talpid3 gene encodes a novel protein essential for Hedgehog signaling. In: Genes & Development. 2006 ; Vol. 20. pp. 1365-1377.
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