The Bvg virulence control system regulates biofilm formation in Bordetella bronchiseptica

Yasuhiko Irie, Seema Mattoo, Ming H Yuk

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Bordetella species utilize the BvgAS (Bordetella virulence gene) two-component signal transduction system to sense the environment and regulate gene expression among at least three phases: a virulent Bvg+ phase, a nonvirulent Bvg- phase, and an intermediate Bvgi phase. Genes expressed in the Bvg+ phase encode known virulence factors, including adhesins such as filamentous hemagglutinin (FHA) and fimbriae, as well as toxins such as the bifunctional adenylate cyclase/hemolysin (ACY). Previous studies showed that in the Bvgi phase, FHA and fimbriae continue to be expressed, but ACY expression is significantly downregulated. In this report, we determine that Bordetella bronchiseptica can form biofilms in vitro and that the generation of biofilm is maximal in the Bvgi phase. We show that FHA is required for maximal biofilm formation and that fimbriae may also contribute to this phenotype. However, expression of ACY inhibits biofilm formation, most likely via interactions with FHA. Therefore, the coordinated regulation of adhesins and ACY expression leads to maximal biofilm formation in the Bvgi phase in B. bronchiseptica.
Original languageEnglish
Pages (from-to)5692-5698
Number of pages7
JournalJournal of Bacteriology
Volume186
Issue number17
DOIs
Publication statusPublished - Sep 2004

Keywords

  • Adaptation, Physiological
  • Adenylate Cyclase Toxin
  • Adhesins, Bacterial
  • Bacterial Adhesion
  • Bacterial Proteins
  • Biofilms
  • Bordetella bronchiseptica
  • Gene Expression Regulation, Bacterial
  • Hemagglutinins
  • Niacin
  • Regulon
  • Signal Transduction
  • Transcription Factors
  • Virulence
  • Virulence Factors, Bordetella

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