Tetrodotoxin-sensitivity of nicotine-evoked dopamine release from rat striatum

D Marshall, L Soliakov, P Redfern, S Wonnacott

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Recent observations from synaptosome preparations have questioned the tetrodotoxin (TTX) insensitivity of nicotine-evoked dopamine release in the striatum, a characteristic previously considered diagnostic of presynaptically located nicotinic acetylcholine receptors (nAChRs). Therefore, we have undertaken a comparison of nicotine-evoked dopamine release in the presence of TTX from the rat striatum in vitro, using synaptosomes and brain slices, and in vivo, using microdialysis. In P2 and Percoll-purified synaptosome preparations, 1.5 mu M TTX partially inhibited nicotine-evoked [H-3]dopamine release by 54% and 37%, respectively, whereas in more intact preparations (brain slices and microdialysis) TTX completely inhibited mecamylamine-sensitive nicotine-stimulated dopamine release. These results suggest that caution should be exercised in the interpretation of TTX sensitivity of nicotine-evoked responses with regard to the location of nAChRs.
Original languageEnglish
Pages (from-to)1531-1536
Number of pages6
JournalNeuropharmacology
Volume35
Issue number11
DOIs
Publication statusPublished - 1996

Keywords

  • calcium
  • nicotinic acetylcholine receptor (nachr)
  • slices
  • stimulation
  • brain slices
  • presynaptic receptors
  • veratridine
  • mecamylamine
  • in vivo microdialysis
  • synaptosomes
  • nucleus
  • brain
  • activation

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