Tetrodotoxin-sensitivity of nicotine-evoked dopamine release from rat striatum

D Marshall, L Soliakov, P Redfern, S Wonnacott

Research output: Contribution to journalArticle

  • 42 Citations

Abstract

Recent observations from synaptosome preparations have questioned the tetrodotoxin (TTX) insensitivity of nicotine-evoked dopamine release in the striatum, a characteristic previously considered diagnostic of presynaptically located nicotinic acetylcholine receptors (nAChRs). Therefore, we have undertaken a comparison of nicotine-evoked dopamine release in the presence of TTX from the rat striatum in vitro, using synaptosomes and brain slices, and in vivo, using microdialysis. In P2 and Percoll-purified synaptosome preparations, 1.5 mu M TTX partially inhibited nicotine-evoked [H-3]dopamine release by 54% and 37%, respectively, whereas in more intact preparations (brain slices and microdialysis) TTX completely inhibited mecamylamine-sensitive nicotine-stimulated dopamine release. These results suggest that caution should be exercised in the interpretation of TTX sensitivity of nicotine-evoked responses with regard to the location of nAChRs.
LanguageEnglish
Pages1531-1536
Number of pages6
JournalNeuropharmacology
Volume35
Issue number11
DOIs
StatusPublished - 1996

Fingerprint

Tetrodotoxin
Nicotine
Dopamine
Synaptosomes
Microdialysis
Nicotinic Receptors
Mecamylamine
Brain

Keywords

  • calcium
  • nicotinic acetylcholine receptor (nachr)
  • slices
  • stimulation
  • brain slices
  • presynaptic receptors
  • veratridine
  • mecamylamine
  • in vivo microdialysis
  • synaptosomes
  • nucleus
  • brain
  • activation

Cite this

Tetrodotoxin-sensitivity of nicotine-evoked dopamine release from rat striatum. / Marshall, D; Soliakov, L; Redfern, P; Wonnacott, S.

In: Neuropharmacology, Vol. 35, No. 11, 1996, p. 1531-1536.

Research output: Contribution to journalArticle

Marshall, D ; Soliakov, L ; Redfern, P ; Wonnacott, S. / Tetrodotoxin-sensitivity of nicotine-evoked dopamine release from rat striatum. In: Neuropharmacology. 1996 ; Vol. 35, No. 11. pp. 1531-1536
@article{1afb00f4ed8b46b7a737f1aeb2df5a65,
title = "Tetrodotoxin-sensitivity of nicotine-evoked dopamine release from rat striatum",
abstract = "Recent observations from synaptosome preparations have questioned the tetrodotoxin (TTX) insensitivity of nicotine-evoked dopamine release in the striatum, a characteristic previously considered diagnostic of presynaptically located nicotinic acetylcholine receptors (nAChRs). Therefore, we have undertaken a comparison of nicotine-evoked dopamine release in the presence of TTX from the rat striatum in vitro, using synaptosomes and brain slices, and in vivo, using microdialysis. In P2 and Percoll-purified synaptosome preparations, 1.5 mu M TTX partially inhibited nicotine-evoked [H-3]dopamine release by 54{\%} and 37{\%}, respectively, whereas in more intact preparations (brain slices and microdialysis) TTX completely inhibited mecamylamine-sensitive nicotine-stimulated dopamine release. These results suggest that caution should be exercised in the interpretation of TTX sensitivity of nicotine-evoked responses with regard to the location of nAChRs.",
keywords = "calcium, nicotinic acetylcholine receptor (nachr), slices, stimulation, brain slices, presynaptic receptors, veratridine, mecamylamine, in vivo microdialysis, synaptosomes, nucleus, brain, activation",
author = "D Marshall and L Soliakov and P Redfern and S Wonnacott",
year = "1996",
doi = "10.1016/S0028-3908(96)00117-7",
language = "English",
volume = "35",
pages = "1531--1536",
journal = "Neuropharmacology",
issn = "0028-3908",
publisher = "Elsevier",
number = "11",

}

TY - JOUR

T1 - Tetrodotoxin-sensitivity of nicotine-evoked dopamine release from rat striatum

AU - Marshall,D

AU - Soliakov,L

AU - Redfern,P

AU - Wonnacott,S

PY - 1996

Y1 - 1996

N2 - Recent observations from synaptosome preparations have questioned the tetrodotoxin (TTX) insensitivity of nicotine-evoked dopamine release in the striatum, a characteristic previously considered diagnostic of presynaptically located nicotinic acetylcholine receptors (nAChRs). Therefore, we have undertaken a comparison of nicotine-evoked dopamine release in the presence of TTX from the rat striatum in vitro, using synaptosomes and brain slices, and in vivo, using microdialysis. In P2 and Percoll-purified synaptosome preparations, 1.5 mu M TTX partially inhibited nicotine-evoked [H-3]dopamine release by 54% and 37%, respectively, whereas in more intact preparations (brain slices and microdialysis) TTX completely inhibited mecamylamine-sensitive nicotine-stimulated dopamine release. These results suggest that caution should be exercised in the interpretation of TTX sensitivity of nicotine-evoked responses with regard to the location of nAChRs.

AB - Recent observations from synaptosome preparations have questioned the tetrodotoxin (TTX) insensitivity of nicotine-evoked dopamine release in the striatum, a characteristic previously considered diagnostic of presynaptically located nicotinic acetylcholine receptors (nAChRs). Therefore, we have undertaken a comparison of nicotine-evoked dopamine release in the presence of TTX from the rat striatum in vitro, using synaptosomes and brain slices, and in vivo, using microdialysis. In P2 and Percoll-purified synaptosome preparations, 1.5 mu M TTX partially inhibited nicotine-evoked [H-3]dopamine release by 54% and 37%, respectively, whereas in more intact preparations (brain slices and microdialysis) TTX completely inhibited mecamylamine-sensitive nicotine-stimulated dopamine release. These results suggest that caution should be exercised in the interpretation of TTX sensitivity of nicotine-evoked responses with regard to the location of nAChRs.

KW - calcium

KW - nicotinic acetylcholine receptor (nachr)

KW - slices

KW - stimulation

KW - brain slices

KW - presynaptic receptors

KW - veratridine

KW - mecamylamine

KW - in vivo microdialysis

KW - synaptosomes

KW - nucleus

KW - brain

KW - activation

UR - http://dx.doi.org/10.1016/S0028-3908(96)00117-7

U2 - 10.1016/S0028-3908(96)00117-7

DO - 10.1016/S0028-3908(96)00117-7

M3 - Article

VL - 35

SP - 1531

EP - 1536

JO - Neuropharmacology

T2 - Neuropharmacology

JF - Neuropharmacology

SN - 0028-3908

IS - 11

ER -