Tetramethylrhodamine self-quenching is a probe of conformational change on the scale of 15 - 25 Å

Paul Girvan; Liming Ying; Charlotte Dodson

doi:10.1039/D4CC04524F

Tetramethylrhodamine self-quenching is a probe of conformational change on the scale of 15 - 25 Å

Paul Girvan, Liming Ying, Charlotte Dodson

Department of Life Sciences

Imperial College London

Research output: Contribution to journal › Article › peer-review

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Abstract

Tetramethylrhodamine (TMR) is a fluorescent dye whose self-quenching has been used as a probe of multiple biological phenomena. We determine the distance-dependence of self-quenching and place bounds on the timescale of TMR dissociation. Our results validate fluorescence self-quenching as an alternative to FRET and enable future assays to be designed with confidence.

Original language	English
Pages (from-to)	5353-5356
Number of pages	4
Journal	Chemical Communications
Volume	61
Issue number	28
Early online date	4 Mar 2025
DOIs	https://doi.org/10.1039/D4CC04524F
Publication status	Published - 4 Mar 2025

Data Availability Statement

Experimental data for the figures in this paper are deposited in Zenodo (DOI: https://doi.org/10.5281/zenodo.12801405).

Funding

This research was funded by the Wellcome Trust, AstraZeneca UK Ltd (RSRO_P71752 to CAD) through the Astra Zeneca/Imperial College London Innovation Fund, the National Institute for Health Research (NIHR) Biomedical Research Centre based at Imperial College Healthcare NHS Trust and Imperial College London (RSRO_P71816 to CAD) and BBSRC (JF20607/2 to LY). The views expressed are those of the author(s) and not necessarily those of the NHS, the NIHR or the Department of Health.

Funders	Funder number
Biotechnology and Biological Sciences Research Council

Access to Document

10.1039/D4CC04524FLicence: CC BY

ChemComm 2025Final published version, 606 KBLicence: CC BY

https://www.biorxiv.org/content/10.1101/2024.08.22.609131v2Licence: CC BY

Cite this

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abstract = "Tetramethylrhodamine (TMR) is a fluorescent dye whose self-quenching has been used as a probe of multiple biological phenomena. We determine the distance-dependence of self-quenching and place bounds on the timescale of TMR dissociation. Our results validate fluorescence self-quenching as an alternative to FRET and enable future assays to be designed with confidence.",

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AB - Tetramethylrhodamine (TMR) is a fluorescent dye whose self-quenching has been used as a probe of multiple biological phenomena. We determine the distance-dependence of self-quenching and place bounds on the timescale of TMR dissociation. Our results validate fluorescence self-quenching as an alternative to FRET and enable future assays to be designed with confidence.

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Tetramethylrhodamine self-quenching is a probe of conformational change on the scale of 15 - 25 Å

Abstract

Data Availability Statement

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The effect of clinical mutations and kinase ligands on conformation and dynamics of protein kinase drug targets

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Data for publication Girvan et al (2025) "Tetramethylrhodamine self-quenching is a probe of conformational change on the scale of 15 – 25 Å"

Cite this

Tetramethylrhodamine self-quenching is a probe of conformational change on the scale of 15 - 25 Å

Abstract

Data Availability Statement

Funding

Access to Document

Other files and links

Fingerprint

Projects

The effect of clinical mutations and kinase ligands on conformation and dynamics of protein kinase drug targets

Value in People Award

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Data for publication Girvan et al (2025) "Tetramethylrhodamine self-quenching is a probe of conformational change on the scale of 15 – 25 Å"

Cite this