Template preparation for rapid PCR in Colletotrichum lindemuthianum

M G Roca, L C Davide, A E Wheals

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3 Citations (Scopus)

Abstract

Isolation of DNA for PCR is time-consuming and involves many reagents. The aim of this work was to optimise a rapid and easy PCR methodology without previous DNA isolation. Different strains of the phytopathogenic fungus Colletotrichum lindemuthianum were used. Protoplasts were generated using lytic enzymes under high incubation temperatures using different methodologies to obtain the template. A rapid (10 minute) methodology was successful for smaller amplicons (
Original languageEnglish
Pages (from-to)8-12
Number of pages5
JournalBrazilian Journal of Microbiology
Volume34
Issue number1
Publication statusPublished - Jan 2003

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    Roca, M. G., Davide, L. C., & Wheals, A. E. (2003). Template preparation for rapid PCR in Colletotrichum lindemuthianum. Brazilian Journal of Microbiology, 34(1), 8-12.