Abstract
Isolation of DNA for PCR is time-consuming and involves many reagents. The aim of this work was to optimise a rapid and easy PCR methodology without previous DNA isolation. Different strains of the phytopathogenic fungus Colletotrichum lindemuthianum were used. Protoplasts were generated using lytic enzymes under high incubation temperatures using different methodologies to obtain the template. A rapid (10 minute) methodology was successful for smaller amplicons (
| Original language | English |
|---|---|
| Pages (from-to) | 8-12 |
| Number of pages | 5 |
| Journal | Brazilian Journal of Microbiology |
| Volume | 34 |
| Issue number | 1 |
| Publication status | Published - Jan 2003 |