Tel/PDGFR beta inhibits self-renewal and directs myelomonocytic differentiation of ES cells

E Dobbin; P M Corrigan; C P Walsh; Melanie J Welham; R W Freeburn; H Wheadon

doi:10.1016/j.leukres.2008.02.007

Tel/PDGFR beta inhibits self-renewal and directs myelomonocytic differentiation of ES cells

E Dobbin, P M Corrigan, C P Walsh, Melanie J Welham, R W Freeburn, H Wheadon

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

7 Citations (SciVal)

Abstract

The leukemic oncogene Tel/PDGFR beta, was inducibly expressed in embryonic stem (ES) cells and the phenotypic and molecular changes occurring during hematopoietic differentiation investigated. Expression of Tel/PDGFR beta resulted in an inability of ES cells to self-renew and caused a significant increase in myelopoiesis with a corresponding decrease in erythropoiesis. Analysis of gene expression patterns indicated a dramatic alteration in the levels of genes associated with self-renewal and differentiation, especially myelomonocytic genes in Tel/PDGFR beta-expressing cells. This study indicates Tel/PDGFR beta drives myelopoiesis by altering expression of genes involved in hematopoiesis and demonstrates the potential of this stem cell system to study oncogene-induced pathogenesis.

Original language	English
Pages (from-to)	1554-1564
Number of pages	11
Journal	Leukemia Research
Volume	32
Issue number	10
DOIs	https://doi.org/10.1016/j.leukres.2008.02.007
Publication status	Published - 2008

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10.1016/j.leukres.2008.02.007

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title = "Tel/PDGFR beta inhibits self-renewal and directs myelomonocytic differentiation of ES cells",

abstract = "The leukemic oncogene Tel/PDGFR beta, was inducibly expressed in embryonic stem (ES) cells and the phenotypic and molecular changes occurring during hematopoietic differentiation investigated. Expression of Tel/PDGFR beta resulted in an inability of ES cells to self-renew and caused a significant increase in myelopoiesis with a corresponding decrease in erythropoiesis. Analysis of gene expression patterns indicated a dramatic alteration in the levels of genes associated with self-renewal and differentiation, especially myelomonocytic genes in Tel/PDGFR beta-expressing cells. This study indicates Tel/PDGFR beta drives myelopoiesis by altering expression of genes involved in hematopoiesis and demonstrates the potential of this stem cell system to study oncogene-induced pathogenesis.",

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T1 - Tel/PDGFR beta inhibits self-renewal and directs myelomonocytic differentiation of ES cells

AU - Dobbin, E

AU - Corrigan, P M

AU - Walsh, C P

AU - Welham, Melanie J

AU - Freeburn, R W

AU - Wheadon, H

PY - 2008

Y1 - 2008

N2 - The leukemic oncogene Tel/PDGFR beta, was inducibly expressed in embryonic stem (ES) cells and the phenotypic and molecular changes occurring during hematopoietic differentiation investigated. Expression of Tel/PDGFR beta resulted in an inability of ES cells to self-renew and caused a significant increase in myelopoiesis with a corresponding decrease in erythropoiesis. Analysis of gene expression patterns indicated a dramatic alteration in the levels of genes associated with self-renewal and differentiation, especially myelomonocytic genes in Tel/PDGFR beta-expressing cells. This study indicates Tel/PDGFR beta drives myelopoiesis by altering expression of genes involved in hematopoiesis and demonstrates the potential of this stem cell system to study oncogene-induced pathogenesis.

AB - The leukemic oncogene Tel/PDGFR beta, was inducibly expressed in embryonic stem (ES) cells and the phenotypic and molecular changes occurring during hematopoietic differentiation investigated. Expression of Tel/PDGFR beta resulted in an inability of ES cells to self-renew and caused a significant increase in myelopoiesis with a corresponding decrease in erythropoiesis. Analysis of gene expression patterns indicated a dramatic alteration in the levels of genes associated with self-renewal and differentiation, especially myelomonocytic genes in Tel/PDGFR beta-expressing cells. This study indicates Tel/PDGFR beta drives myelopoiesis by altering expression of genes involved in hematopoiesis and demonstrates the potential of this stem cell system to study oncogene-induced pathogenesis.

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DO - 10.1016/j.leukres.2008.02.007

M3 - Article

SN - 0145-2126

VL - 32

SP - 1554

EP - 1564

JO - Leukemia Research

JF - Leukemia Research

IS - 10

ER -

Tel/PDGFR beta inhibits self-renewal and directs myelomonocytic differentiation of ES cells

Abstract

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