Systematic global analysis of genes encoding protein phosphatases in Aspergillus fumigatus

Lizziane K. Winkelströter, Stephen K. Dolan, Thaila Fernanda dos Reis, Vinícius Leite Pedro Bom, Patrícia Alves de Castro, Daisuke Hagiwara, Raneem Alowni, Gary W. Jones, Sean Doyle, Neil Andrew Brown, Gustavo H. Goldman

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

Aspergillus fumigatus is a fungal pathogen that causes several invasive and noninvasive diseases named aspergillosis. This disease is generally regarded as multifactorial, considering that several pathogenicity determinants are present during the establishment of this illness. It is necessary to obtain an increased knowledge of how, and which, A. fumigatus signal transduction pathways are engaged in the regulation of these processes. Protein phosphatases are essential to several signal transduction pathways. We identified 32 phosphatase catalytic subunit-encoding genes in A. fumigatus, of which we were able to construct 24 viable deletion mutants. The role of nine phosphatase mutants in the HOG (high osmolarity glycerol response) pathway was evaluated by measuring phosphorylation of the p38 MAPK (SakA) and expression of osmo-dependent genes. We were also able to identify 11 phosphatases involved in iron assimilation, six that are related to gliotoxin resistance, and three implicated in gliotoxin production. These results present the creation of a fundamental resource for the study of signaling in A. fumigatus and its implications in the regulation of pathogenicity determinants and virulence in this important pathogen.

Original languageEnglish
Pages (from-to)1525-1539
Number of pages15
JournalG3: Genes, Genomes, Genetics
Volume5
Issue number7
DOIs
Publication statusPublished - 1 Jul 2015

Keywords

  • Aspergillus fumigatus
  • Gliotoxin
  • HOG phosphatases
  • Iron metabolism
  • Protein phosphatases

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

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