Synthetic microbial consortia bioprocessing integrated with pyrolysis for efficient conversion of cellulose to valuables

Chandrakant Joshi; Mahesh Kumar; Martyn Bennett; Jyotika Thakur; David J. Leak; Swati Sharma; Neil MacKinnon; Shyam Kumar Masakapalli

doi:10.1016/j.biteb.2022.101316

Synthetic microbial consortia bioprocessing integrated with pyrolysis for efficient conversion of cellulose to valuables

Chandrakant Joshi, Mahesh Kumar, Martyn Bennett, Jyotika Thakur, David J. Leak, Swati Sharma, Neil MacKinnon, Shyam Kumar Masakapalli

Research output: Contribution to journal › Article › peer-review

7 Citations (SciVal)

Abstract

Synthetic microbial consortium (SynCONS) based bioprocessing integrated with pyrolysis is successfully demonstrated as an advance in our quest to convert cellulose to valuables. Comparative metabolic analysis of multiple microbes allowed robust design of SynCONS aimed at consolidated bioprocessing of cellulose to ethanol and lactate. The microbial partners of Trichoderma reesei - Parageobacillus thermoglucosidasius (fungal-bacterial) and Thermobifida fusca - P. thermoglucosidasius (bacterial-bacterial, thermophilic) showed promising cellulose degradation and total yields of 9 % and 23 % respectively. Further, higher ethanol yields were achieved in thermophilic SynCONS using engineered fermentative partner P. thermoglucosidasius TM242 (33 %) or pure cellulases for saccharification (51 %). Finally, pyrolyzing the residual cellulose and microbial biomass resulted in carbon material with desirable physicochemical properties as revealed by Scanning Electron Microscopy, X-ray Diffraction and Raman spectroscopy. Overall, the integration of SynCONS bioprocessing with pyrolysis confirmed a promising strategy for conversion of cellulose to chemicals, biofuels, and carbon suitable for several industrial applications.

Original language	English
Article number	101316
Journal	Bioresource Technology Reports
Volume	21
Early online date	28 Dec 2022
DOIs	https://doi.org/10.1016/j.biteb.2022.101316
Publication status	Published - 28 Feb 2023

Bibliographical note

Funding Information:
This work was supported by project BioPEC, jointly funded by the Department of Biotechnology (DBT), India (Ref. No. BT/IN/BMBF-Germany/29/SKM/2016-17 ) and the Federal Ministry of Education and Research (BMBF), Germany (Ref. No. 01DQ17014 ). Chandrakant and Jyotika thank the Ministry of Education, and IIT Mandi, India for doctoral research fellowship. Chandrakant and Jyotika are thankful to BioX centre and Advanced Materials Research Center, IIT Mandi for research facilities.

Funding Information:
This work was supported by project BioPEC, jointly funded by the Department of Biotechnology (DBT), India (Ref. No. BT/IN/BMBF-Germany/29/SKM/2016-17) and the Federal Ministry of Education and Research (BMBF), Germany (Ref. No. 01DQ17014). Chandrakant and Jyotika thank the Ministry of Education, and IIT Mandi, India for doctoral research fellowship. Chandrakant and Jyotika are thankful to BioX centre and Advanced Materials Research Center, IIT Mandi for research facilities.

Publisher Copyright:
© 2022 Elsevier Ltd

Funding

This work was supported by project BioPEC, jointly funded by the Department of Biotechnology (DBT), India (Ref. No. BT/IN/BMBF-Germany/29/SKM/2016-17 ) and the Federal Ministry of Education and Research (BMBF), Germany (Ref. No. 01DQ17014 ). Chandrakant and Jyotika thank the Ministry of Education, and IIT Mandi, India for doctoral research fellowship. Chandrakant and Jyotika are thankful to BioX centre and Advanced Materials Research Center, IIT Mandi for research facilities. This work was supported by project BioPEC, jointly funded by the Department of Biotechnology (DBT), India (Ref. No. BT/IN/BMBF-Germany/29/SKM/2016-17) and the Federal Ministry of Education and Research (BMBF), Germany (Ref. No. 01DQ17014). Chandrakant and Jyotika thank the Ministry of Education, and IIT Mandi, India for doctoral research fellowship. Chandrakant and Jyotika are thankful to BioX centre and Advanced Materials Research Center, IIT Mandi for research facilities.

Keywords

Biofuels
Bioreactors
Consolidated bioprocessing
Pyrolyzed carbon
Synthetic microbial consortia

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.biteb.2022.101316

Cite this

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title = "Synthetic microbial consortia bioprocessing integrated with pyrolysis for efficient conversion of cellulose to valuables",

abstract = "Synthetic microbial consortium (SynCONS) based bioprocessing integrated with pyrolysis is successfully demonstrated as an advance in our quest to convert cellulose to valuables. Comparative metabolic analysis of multiple microbes allowed robust design of SynCONS aimed at consolidated bioprocessing of cellulose to ethanol and lactate. The microbial partners of Trichoderma reesei - Parageobacillus thermoglucosidasius (fungal-bacterial) and Thermobifida fusca - P. thermoglucosidasius (bacterial-bacterial, thermophilic) showed promising cellulose degradation and total yields of 9 % and 23 % respectively. Further, higher ethanol yields were achieved in thermophilic SynCONS using engineered fermentative partner P. thermoglucosidasius TM242 (33 %) or pure cellulases for saccharification (51 %). Finally, pyrolyzing the residual cellulose and microbial biomass resulted in carbon material with desirable physicochemical properties as revealed by Scanning Electron Microscopy, X-ray Diffraction and Raman spectroscopy. Overall, the integration of SynCONS bioprocessing with pyrolysis confirmed a promising strategy for conversion of cellulose to chemicals, biofuels, and carbon suitable for several industrial applications.",

keywords = "Biofuels, Bioreactors, Consolidated bioprocessing, Pyrolyzed carbon, Synthetic microbial consortia",

author = "Chandrakant Joshi and Mahesh Kumar and Martyn Bennett and Jyotika Thakur and Leak, {David J.} and Swati Sharma and Neil MacKinnon and Masakapalli, {Shyam Kumar}",

note = "Funding Information: This work was supported by project BioPEC, jointly funded by the Department of Biotechnology (DBT), India (Ref. No. BT/IN/BMBF-Germany/29/SKM/2016-17 ) and the Federal Ministry of Education and Research (BMBF), Germany (Ref. No. 01DQ17014 ). Chandrakant and Jyotika thank the Ministry of Education, and IIT Mandi, India for doctoral research fellowship. Chandrakant and Jyotika are thankful to BioX centre and Advanced Materials Research Center, IIT Mandi for research facilities. Funding Information: This work was supported by project BioPEC, jointly funded by the Department of Biotechnology (DBT), India (Ref. No. BT/IN/BMBF-Germany/29/SKM/2016-17) and the Federal Ministry of Education and Research (BMBF), Germany (Ref. No. 01DQ17014). Chandrakant and Jyotika thank the Ministry of Education, and IIT Mandi, India for doctoral research fellowship. Chandrakant and Jyotika are thankful to BioX centre and Advanced Materials Research Center, IIT Mandi for research facilities. Publisher Copyright: {\textcopyright} 2022 Elsevier Ltd",

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AU - Joshi, Chandrakant

AU - Kumar, Mahesh

AU - Bennett, Martyn

AU - Thakur, Jyotika

AU - Leak, David J.

AU - Sharma, Swati

AU - MacKinnon, Neil

AU - Masakapalli, Shyam Kumar

N1 - Funding Information: This work was supported by project BioPEC, jointly funded by the Department of Biotechnology (DBT), India (Ref. No. BT/IN/BMBF-Germany/29/SKM/2016-17 ) and the Federal Ministry of Education and Research (BMBF), Germany (Ref. No. 01DQ17014 ). Chandrakant and Jyotika thank the Ministry of Education, and IIT Mandi, India for doctoral research fellowship. Chandrakant and Jyotika are thankful to BioX centre and Advanced Materials Research Center, IIT Mandi for research facilities. Funding Information: This work was supported by project BioPEC, jointly funded by the Department of Biotechnology (DBT), India (Ref. No. BT/IN/BMBF-Germany/29/SKM/2016-17) and the Federal Ministry of Education and Research (BMBF), Germany (Ref. No. 01DQ17014). Chandrakant and Jyotika thank the Ministry of Education, and IIT Mandi, India for doctoral research fellowship. Chandrakant and Jyotika are thankful to BioX centre and Advanced Materials Research Center, IIT Mandi for research facilities. Publisher Copyright: © 2022 Elsevier Ltd

PY - 2023/2/28

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N2 - Synthetic microbial consortium (SynCONS) based bioprocessing integrated with pyrolysis is successfully demonstrated as an advance in our quest to convert cellulose to valuables. Comparative metabolic analysis of multiple microbes allowed robust design of SynCONS aimed at consolidated bioprocessing of cellulose to ethanol and lactate. The microbial partners of Trichoderma reesei - Parageobacillus thermoglucosidasius (fungal-bacterial) and Thermobifida fusca - P. thermoglucosidasius (bacterial-bacterial, thermophilic) showed promising cellulose degradation and total yields of 9 % and 23 % respectively. Further, higher ethanol yields were achieved in thermophilic SynCONS using engineered fermentative partner P. thermoglucosidasius TM242 (33 %) or pure cellulases for saccharification (51 %). Finally, pyrolyzing the residual cellulose and microbial biomass resulted in carbon material with desirable physicochemical properties as revealed by Scanning Electron Microscopy, X-ray Diffraction and Raman spectroscopy. Overall, the integration of SynCONS bioprocessing with pyrolysis confirmed a promising strategy for conversion of cellulose to chemicals, biofuels, and carbon suitable for several industrial applications.

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KW - Pyrolyzed carbon

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Synthetic microbial consortia bioprocessing integrated with pyrolysis for efficient conversion of cellulose to valuables

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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HPLC System

Cite this

Synthetic microbial consortia bioprocessing integrated with pyrolysis for efficient conversion of cellulose to valuables

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Equipment

HPLC System

Cite this