Synthetic microbial consortia bioprocessing integrated with pyrolysis for efficient conversion of cellulose to valuables

Chandrakant Joshi, Mahesh Kumar, Martyn Bennett, Jyotika Thakur, David J. Leak, Swati Sharma, Neil MacKinnon, Shyam Kumar Masakapalli

Research output: Contribution to journalArticlepeer-review

6 Citations (SciVal)

Abstract

Synthetic microbial consortium (SynCONS) based bioprocessing integrated with pyrolysis is successfully demonstrated as an advance in our quest to convert cellulose to valuables. Comparative metabolic analysis of multiple microbes allowed robust design of SynCONS aimed at consolidated bioprocessing of cellulose to ethanol and lactate. The microbial partners of Trichoderma reesei - Parageobacillus thermoglucosidasius (fungal-bacterial) and Thermobifida fusca - P. thermoglucosidasius (bacterial-bacterial, thermophilic) showed promising cellulose degradation and total yields of 9 % and 23 % respectively. Further, higher ethanol yields were achieved in thermophilic SynCONS using engineered fermentative partner P. thermoglucosidasius TM242 (33 %) or pure cellulases for saccharification (51 %). Finally, pyrolyzing the residual cellulose and microbial biomass resulted in carbon material with desirable physicochemical properties as revealed by Scanning Electron Microscopy, X-ray Diffraction and Raman spectroscopy. Overall, the integration of SynCONS bioprocessing with pyrolysis confirmed a promising strategy for conversion of cellulose to chemicals, biofuels, and carbon suitable for several industrial applications.

Original languageEnglish
Article number101316
JournalBioresource Technology Reports
Volume21
Early online date28 Dec 2022
DOIs
Publication statusPublished - 28 Feb 2023

Bibliographical note

Funding Information:
This work was supported by project BioPEC, jointly funded by the Department of Biotechnology (DBT), India (Ref. No. BT/IN/BMBF-Germany/29/SKM/2016-17 ) and the Federal Ministry of Education and Research (BMBF), Germany (Ref. No. 01DQ17014 ). Chandrakant and Jyotika thank the Ministry of Education, and IIT Mandi, India for doctoral research fellowship. Chandrakant and Jyotika are thankful to BioX centre and Advanced Materials Research Center, IIT Mandi for research facilities.

Funding Information:
This work was supported by project BioPEC, jointly funded by the Department of Biotechnology (DBT), India (Ref. No. BT/IN/BMBF-Germany/29/SKM/2016-17) and the Federal Ministry of Education and Research (BMBF), Germany (Ref. No. 01DQ17014). Chandrakant and Jyotika thank the Ministry of Education, and IIT Mandi, India for doctoral research fellowship. Chandrakant and Jyotika are thankful to BioX centre and Advanced Materials Research Center, IIT Mandi for research facilities.

Publisher Copyright:
© 2022 Elsevier Ltd

Keywords

  • Biofuels
  • Bioreactors
  • Consolidated bioprocessing
  • Pyrolyzed carbon
  • Synthetic microbial consortia

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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