Synthesis, radiolabelling and in vitro imaging of multifunctional nanoceramics

Marina Lledos, Vincenzo Mirabello, Sophia Sarpaki, Haobo Ge, Hubert Jakub Smugowski, Laurence Carroll, Eric O. Aboagye, Franklin I. Aigbirhio, Stanley W Botchway, Jonathan R Dilworth, David Gonzalez Calatayud, Pawel Plucinski, Gareth Price, Sofia Pascu

Research output: Contribution to journalArticle

2 Citations (Scopus)
15 Downloads (Pure)

Abstract

Molecular imaging has become a powerful technique in preclinical, clinical research and the diagnosis of many diseases. In this work, we address for the first time the synthetic challenges in achieving lab-scale, batch-to-batch reproducible 64Cu and 68Ga radiolabelled MNPs for imaging purposes. Composite nanoparticles incorporating magnetic iron oxide cores with luminescent quantum dots were simultaneously encapsulated within a thin silica shell, yielding water-dispersible, biocompatible and luminescent NPs. Novel, scalable surface modification protocols to attach the radioisotopes 64Cu (t1/2 = 12.7 h) and 68Ga (t1/2 = 68 min) in high yields are reported which are compatible with the time frame of radiolabelling. Confocal and fluorescence lifetime imaging studies confirm the uptake of the encapsulated imaging agents and their cytoplasmic localization in prostate cancer (PC-3) cells. Cellular viability assays show that the biocompatibility of the system is improved when the fluorophores are encapsulated within a silica shell. The functional and biocompatible SiO2 matrix represents an ideal platform for the incorporation of 64Cu and 68Ga radioisotopes with high radiolabelling incorporation.
Original languageEnglish
Pages (from-to)361-372
JournalChemNanoMat
Volume4
Issue number4
Early online date8 Feb 2018
DOIs
Publication statusPublished - 1 Apr 2018

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Imaging techniques
Radioisotopes
Silicon Dioxide
Silica
Molecular imaging
Fluorophores
Iron oxides
Biocompatibility
Semiconductor quantum dots
Surface treatment
Assays
Fluorescence
Nanoparticles
Water
Composite materials
ferric oxide

Cite this

Synthesis, radiolabelling and in vitro imaging of multifunctional nanoceramics. / Lledos, Marina; Mirabello, Vincenzo; Sarpaki, Sophia; Ge, Haobo; Smugowski, Hubert Jakub; Carroll, Laurence; Aboagye, Eric O.; Aigbirhio, Franklin I.; Botchway, Stanley W; Dilworth, Jonathan R; Gonzalez Calatayud, David; Plucinski, Pawel; Price, Gareth; Pascu, Sofia.

In: ChemNanoMat, Vol. 4, No. 4, 01.04.2018, p. 361-372.

Research output: Contribution to journalArticle

Lledos, M, Mirabello, V, Sarpaki, S, Ge, H, Smugowski, HJ, Carroll, L, Aboagye, EO, Aigbirhio, FI, Botchway, SW, Dilworth, JR, Gonzalez Calatayud, D, Plucinski, P, Price, G & Pascu, S 2018, 'Synthesis, radiolabelling and in vitro imaging of multifunctional nanoceramics', ChemNanoMat, vol. 4, no. 4, pp. 361-372. https://doi.org/10.1002/cnma.201700378
Lledos M, Mirabello V, Sarpaki S, Ge H, Smugowski HJ, Carroll L et al. Synthesis, radiolabelling and in vitro imaging of multifunctional nanoceramics. ChemNanoMat. 2018 Apr 1;4(4):361-372. https://doi.org/10.1002/cnma.201700378
Lledos, Marina ; Mirabello, Vincenzo ; Sarpaki, Sophia ; Ge, Haobo ; Smugowski, Hubert Jakub ; Carroll, Laurence ; Aboagye, Eric O. ; Aigbirhio, Franklin I. ; Botchway, Stanley W ; Dilworth, Jonathan R ; Gonzalez Calatayud, David ; Plucinski, Pawel ; Price, Gareth ; Pascu, Sofia. / Synthesis, radiolabelling and in vitro imaging of multifunctional nanoceramics. In: ChemNanoMat. 2018 ; Vol. 4, No. 4. pp. 361-372.
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