New diazirine based bis-glucose derivatives for tagging glucose transporters have been synthesised. These included two biotinylated compounds linked either by an aminocaproate or by a cleavable dithiol link. These compounds have been derivatised via a key skeleton compound that can be easily used for introduction of additional tags. Studies on the erythrocyte glucose transporter (GLUT1) and the insulin-stimulated adipose cell transporter (GLUT-I) have revealed the biotinylated photoreactive bis-glucose compounds are effective labelling reagents, (C) 2001 Elsevier Science Ltd. All rights reserved.
|Number of pages||9|
|Publication status||Published - 2001|
Hashimoto, M., Hatanaka, Y., Yang, J., Dhesi, J., & Holman, G. D. (2001). Synthesis of biotinylated bis(D-glucose) derivatives for glucose transporter photoaffinity labelling. Carbohydrate Research, 331(2), 119-127.