Synthesis of biotinylated bis(D-glucose) derivatives for glucose transporter photoaffinity labelling

M Hashimoto, Y Hatanaka, J Yang, J Dhesi, G D Holman

Research output: Contribution to journalArticle

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Abstract

New diazirine based bis-glucose derivatives for tagging glucose transporters have been synthesised. These included two biotinylated compounds linked either by an aminocaproate or by a cleavable dithiol link. These compounds have been derivatised via a key skeleton compound that can be easily used for introduction of additional tags. Studies on the erythrocyte glucose transporter (GLUT1) and the insulin-stimulated adipose cell transporter (GLUT-I) have revealed the biotinylated photoreactive bis-glucose compounds are effective labelling reagents, (C) 2001 Elsevier Science Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)119-127
Number of pages9
JournalCarbohydrate Research
Volume331
Issue number2
Publication statusPublished - 2001

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Facilitative Glucose Transport Proteins
Labeling
Glucose Transporter Type 1
Diazomethane
Derivatives
Glucose
Skeleton
Insulin
dithiol

Cite this

Synthesis of biotinylated bis(D-glucose) derivatives for glucose transporter photoaffinity labelling. / Hashimoto, M; Hatanaka, Y; Yang, J; Dhesi, J; Holman, G D.

In: Carbohydrate Research, Vol. 331, No. 2, 2001, p. 119-127.

Research output: Contribution to journalArticle

Hashimoto, M ; Hatanaka, Y ; Yang, J ; Dhesi, J ; Holman, G D. / Synthesis of biotinylated bis(D-glucose) derivatives for glucose transporter photoaffinity labelling. In: Carbohydrate Research. 2001 ; Vol. 331, No. 2. pp. 119-127.
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