Switching protein metalloporphyrin binding specificity by design from iron to fluorogenic zinc

Benjamin J Bowen; Adam R McGarrity; Jenn-Yeu A Szeto; Christopher R Pudney; D Dafydd Jones

doi:10.1039/d0cc00596g

Switching protein metalloporphyrin binding specificity by design from iron to fluorogenic zinc

Benjamin J Bowen, Adam R McGarrity, Jenn-Yeu A Szeto, Christopher R Pudney, D Dafydd Jones

Research output: Contribution to journal › Article › peer-review

2 Citations (SciVal)

Abstract

Metalloporphyrins play important roles in areas ranging from biology to nanoscience. Using computational design, we converted metalloporphyrin specificity of cytochrome b562 from iron to fluorogenic zinc. The new variant had a near total preference for zinc representing a switch in specificity, which greatly enhanced the negligible aqueous fluorescence of free ZnPP in vitro and in vivo.

Original language	English
Pages (from-to)	4308-4311
Number of pages	4
Journal	Chemical communications (Cambridge, England)
Volume	56
Issue number	31
Early online date	12 Mar 2020
DOIs	https://doi.org/10.1039/d0cc00596g
Publication status	Published - 21 Apr 2020

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/d0cc00596g

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abstract = "Metalloporphyrins play important roles in areas ranging from biology to nanoscience. Using computational design, we converted metalloporphyrin specificity of cytochrome b562 from iron to fluorogenic zinc. The new variant had a near total preference for zinc representing a switch in specificity, which greatly enhanced the negligible aqueous fluorescence of free ZnPP in vitro and in vivo.",

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AU - Bowen, Benjamin J

AU - McGarrity, Adam R

AU - Szeto, Jenn-Yeu A

AU - Pudney, Christopher R

AU - Jones, D Dafydd

PY - 2020/4/21

Y1 - 2020/4/21

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AB - Metalloporphyrins play important roles in areas ranging from biology to nanoscience. Using computational design, we converted metalloporphyrin specificity of cytochrome b562 from iron to fluorogenic zinc. The new variant had a near total preference for zinc representing a switch in specificity, which greatly enhanced the negligible aqueous fluorescence of free ZnPP in vitro and in vivo.

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DO - 10.1039/d0cc00596g

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EP - 4311

JO - Chemical communications (Cambridge, England)

JF - Chemical communications (Cambridge, England)

SN - 1359-7345

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Switching protein metalloporphyrin binding specificity by design from iron to fluorogenic zinc

Abstract

ASJC Scopus subject areas

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