Switching protein metalloporphyrin binding specificity by design from iron to fluorogenic zinc

Benjamin J Bowen, Adam R McGarrity, Jenn-Yeu A Szeto, Christopher R Pudney, D Dafydd Jones

Research output: Contribution to journalArticlepeer-review

Abstract

Metalloporphyrins play important roles in areas ranging from biology to nanoscience. Using computational design, we converted metalloporphyrin specificity of cytochrome b562 from iron to fluorogenic zinc. The new variant had a near total preference for zinc representing a switch in specificity, which greatly enhanced the negligible aqueous fluorescence of free ZnPP in vitro and in vivo.

Original languageEnglish
Pages (from-to)4308-4311
Number of pages4
JournalChemical communications (Cambridge, England)
Volume56
Issue number31
Early online date12 Mar 2020
DOIs
Publication statusPublished - 21 Apr 2020

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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