Switching of electrochemical characteristics of redox protein upon specific biomolecular interactions

Man Yi Ho, Sarah A. Goodchild, Pedro Estrela, Daping Chu, Piero Migliorato

Research output: Contribution to journalArticle

3 Citations (Scopus)
99 Downloads (Pure)

Abstract

Detection of specific protein analytes is a technique widely used in disease diagnosis. Central to this approach is the fabrication of a sensing platform displaying a functional recognition element specific for the analyte targeted for detection. The most commonly utilised type of recognition element used for this purpose are antibodies. However direct generation of surfaces with high functional binding activity when using antibodies frequently presents a challenge, due to the conformational changes undergone by these molecules when physisorbed on a solid surface and/or variable activity when immobilized by covalent coupling techniques. Here, we present a novel label-free protein sensing platform based on a simplified and standardized immobilization process. The platform consists of self-assembled redox protein; Azurin (Az), that acts as scaffold, while sensing specificity is achieved through receptors that are coupled with chemical groups available on the surface of the Az protein. The redox activity of the Az within the sensing surface enables a label-free electrochemical detection method that can be readily miniaturized. We have observed a significant change in the electrochemical characteristics of the assay, upon a specific molecular interaction. A corresponding new model is also developed that can aid the future development of redox based bio-sensing techniques.
Original languageEnglish
Pages (from-to)6118-6121
JournalAnalyst
Volume139
Issue number23
Early online date6 Oct 2014
DOIs
Publication statusPublished - 7 Dec 2014

Fingerprint

Azurin
Oxidation-Reduction
Proteins
protein
Antibodies
antibody
Labels
Immobilization
Molecular interactions
Membrane Proteins
Scaffolds (biology)
detection method
Scaffolds
immobilization
Assays
assay
Fabrication
Molecules

Keywords

  • Biosensors

Cite this

Switching of electrochemical characteristics of redox protein upon specific biomolecular interactions. / Ho, Man Yi; Goodchild, Sarah A.; Estrela, Pedro; Chu, Daping; Migliorato, Piero.

In: Analyst, Vol. 139, No. 23, 07.12.2014, p. 6118-6121.

Research output: Contribution to journalArticle

Ho, Man Yi ; Goodchild, Sarah A. ; Estrela, Pedro ; Chu, Daping ; Migliorato, Piero. / Switching of electrochemical characteristics of redox protein upon specific biomolecular interactions. In: Analyst. 2014 ; Vol. 139, No. 23. pp. 6118-6121.
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