Surface modified cellulose scaffolds for tissue engineering

James C. Courtenay, Marcus A. Johns, Fernando Galembeck, Christoph Deneke, Evandro M. Lanzoni, Carlos A. Costa, Janet L. Scott, Ram I. Sharma

Research output: Contribution to journalArticle

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Abstract

We report the ability of cellulose to support cells without the use of matrix ligands on the surface of the material, thus creating a two-component system for tissue engineering of cells and materials. Sheets of bacterial cellulose, grown from a culture medium containing Acetobacter organism were chemically modified with glycidyltrimethylammonium chloride or by oxidation with sodium hypochlorite in the presence of sodium bromide and 2,2,6,6-tetramethylpipiridine 1-oxyl radical to introduce a positive, or negative, charge, respectively. This modification process did not degrade the mechanical properties of the bulk material, but grafting of a positively charged moiety to the cellulose surface (cationic cellulose) increased cell attachment by 70% compared to unmodified cellulose, while negatively charged, oxidised cellulose films (anionic cellulose), showed low levels of cell attachment comparable to those seen for unmodified cellulose. Only a minimal level of cationic surface derivitisation (ca 3% degree of substitution) was required for increased cell attachment and no mediating proteins were required. Cell adhesion studies exhibited the same trends as the attachment studies, while the mean cell area and aspect ratio was highest on the cationic surfaces. Overall, we demonstrated the utility of positively charged bacterial cellulose in tissue engineering in the absence of proteins for cell attachment.

LanguageEnglish
Pages253-267
Number of pages15
JournalCellulose
Volume24
Issue number1
DOIs
StatusPublished - Jan 2017

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Scaffolds (biology)
Tissue engineering
Cellulose
oxidized cellulose
Acetobacter
Sodium
Cellulose films
Proteins
Sodium Hypochlorite
Cell adhesion
Culture Media
Aspect ratio
Substitution reactions
Ligands
Oxidation
Mechanical properties

Keywords

  • Bacterial cellulose
  • Cell adhesion
  • Surface modification
  • Tissue engineering scaffolds

Cite this

Courtenay, J. C., Johns, M. A., Galembeck, F., Deneke, C., Lanzoni, E. M., Costa, C. A., ... Sharma, R. I. (2017). Surface modified cellulose scaffolds for tissue engineering. DOI: 10.1007/s10570-016-1111-y

Surface modified cellulose scaffolds for tissue engineering. / Courtenay, James C.; Johns, Marcus A.; Galembeck, Fernando; Deneke, Christoph; Lanzoni, Evandro M.; Costa, Carlos A.; Scott, Janet L.; Sharma, Ram I.

In: Cellulose, Vol. 24, No. 1, 01.2017, p. 253-267.

Research output: Contribution to journalArticle

Courtenay, JC, Johns, MA, Galembeck, F, Deneke, C, Lanzoni, EM, Costa, CA, Scott, JL & Sharma, RI 2017, 'Surface modified cellulose scaffolds for tissue engineering' Cellulose, vol. 24, no. 1, pp. 253-267. DOI: 10.1007/s10570-016-1111-y
Courtenay JC, Johns MA, Galembeck F, Deneke C, Lanzoni EM, Costa CA et al. Surface modified cellulose scaffolds for tissue engineering. Cellulose. 2017 Jan;24(1):253-267. Available from, DOI: 10.1007/s10570-016-1111-y
Courtenay, James C. ; Johns, Marcus A. ; Galembeck, Fernando ; Deneke, Christoph ; Lanzoni, Evandro M. ; Costa, Carlos A. ; Scott, Janet L. ; Sharma, Ram I./ Surface modified cellulose scaffolds for tissue engineering. In: Cellulose. 2017 ; Vol. 24, No. 1. pp. 253-267
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