Sub millimetre flexible fibre probe for background and fluorescence free Raman spectroscopy

Stephanos Yerolatsitis; András Kufcsák; Katjana Ehrlich; Harry A.C. Wood; Susan Fernandes; Tom Quinn; Vikki Young; Irene Young; Katie Hamilton; Ahsan R. Akram; Robert R. Thomson; Keith Finlayson; Kevin Dhaliwal; J. M. Stone

doi:10.1002/jbio.202000488

Sub millimetre flexible fibre probe for background and fluorescence free Raman spectroscopy

Stephanos Yerolatsitis, András Kufcsák, Katjana Ehrlich, Harry A.C. Wood, Susan Fernandes, Tom Quinn, Vikki Young, Irene Young, Katie Hamilton, Ahsan R. Akram, Robert R. Thomson, Keith Finlayson, Kevin Dhaliwal, J. M. Stone

Research output: Contribution to journal › Article › peer-review

6 Citations (SciVal)

Abstract

Using the shifted-excitation Raman difference spectroscopy technique and an optical fibre featuring a negative curvature excitation core and a coaxial ring of high numerical aperture collection cores, we have developed a portable, background and fluorescence free, endoscopic Raman probe. The probe consists of a single fibre with a diameter of less than 0.25 mm packaged in a sub-millimetre tubing, making it compatible with standard bronchoscopes. The Raman excitation light in the fibre is guided in air and therefore interacts little with silica, enabling an almost background free transmission of the excitation light. In addition, we used the shifted-excitation Raman difference spectroscopy technique and a tunable 785 nm laser to separate the fluorescence and the Raman spectrum from highly fluorescent samples, demonstrating the suitability of the probe for biomedical applications. Using this probe we also acquired fluorescence free human lung tissue data.

Original language	English
Article number	e202000488
Journal	Journal of Biophotonics
Volume	14
Issue number	10
Early online date	15 Apr 2021
DOIs	https://doi.org/10.1002/jbio.202000488
Publication status	Published - 31 Oct 2021

Bibliographical note

Funding Information:
The authors thank the BioResource for access to tissue (NHS Lothian BioResource, Scotland Research Ethics Service, reference 15/ES/0094). James M. Stone, Stephanos Yerolatsitis and Harry A. C. Wood are supported through an EPSRC fellowship (EP/S001123/1). Susan Fernandes is supported through an MRC fellowship (MR/R017794/1). Ahsan R. Akram is supported by a CRUK Clinician Scientist Fellowship (A24867).

Funding

The authors thank the BioResource for access to tissue (NHS Lothian BioResource, Scotland Research Ethics Service, reference 15/ES/0094). James M. Stone, Stephanos Yerolatsitis and Harry A. C. Wood are supported through an EPSRC fellowship (EP/S001123/1). Susan Fernandes is supported through an MRC fellowship (MR/R017794/1). Ahsan R. Akram is supported by a CRUK Clinician Scientist Fellowship (A24867).

Keywords

hollow-core fibre
Raman fibre probe
Raman spectroscopy
SERDS

ASJC Scopus subject areas

General Chemistry
General Materials Science
General Biochemistry,Genetics and Molecular Biology
General Engineering
General Physics and Astronomy

Access to Document

10.1002/jbio.202000488Licence: CC BY

Cite this

Yerolatsitis, S., Kufcsák, A., Ehrlich, K., Wood, H. A. C., Fernandes, S., Quinn, T., Young, V., Young, I., Hamilton, K., Akram, A. R., Thomson, R. R., Finlayson, K., Dhaliwal, K., & Stone, J. M. (2021). Sub millimetre flexible fibre probe for background and fluorescence free Raman spectroscopy. Journal of Biophotonics, 14(10), Article e202000488. https://doi.org/10.1002/jbio.202000488

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abstract = "Using the shifted-excitation Raman difference spectroscopy technique and an optical fibre featuring a negative curvature excitation core and a coaxial ring of high numerical aperture collection cores, we have developed a portable, background and fluorescence free, endoscopic Raman probe. The probe consists of a single fibre with a diameter of less than 0.25 mm packaged in a sub-millimetre tubing, making it compatible with standard bronchoscopes. The Raman excitation light in the fibre is guided in air and therefore interacts little with silica, enabling an almost background free transmission of the excitation light. In addition, we used the shifted-excitation Raman difference spectroscopy technique and a tunable 785 nm laser to separate the fluorescence and the Raman spectrum from highly fluorescent samples, demonstrating the suitability of the probe for biomedical applications. Using this probe we also acquired fluorescence free human lung tissue data.",

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author = "Stephanos Yerolatsitis and Andr{\'a}s Kufcs{\'a}k and Katjana Ehrlich and Wood, {Harry A.C.} and Susan Fernandes and Tom Quinn and Vikki Young and Irene Young and Katie Hamilton and Akram, {Ahsan R.} and Thomson, {Robert R.} and Keith Finlayson and Kevin Dhaliwal and Stone, {J. M.}",

note = "Funding Information: The authors thank the BioResource for access to tissue (NHS Lothian BioResource, Scotland Research Ethics Service, reference 15/ES/0094). James M. Stone, Stephanos Yerolatsitis and Harry A. C. Wood are supported through an EPSRC fellowship (EP/S001123/1). Susan Fernandes is supported through an MRC fellowship (MR/R017794/1). Ahsan R. Akram is supported by a CRUK Clinician Scientist Fellowship (A24867). ",

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AU - Yerolatsitis, Stephanos

AU - Kufcsák, András

AU - Ehrlich, Katjana

AU - Wood, Harry A.C.

AU - Fernandes, Susan

AU - Quinn, Tom

AU - Young, Vikki

AU - Young, Irene

AU - Hamilton, Katie

AU - Akram, Ahsan R.

AU - Thomson, Robert R.

AU - Finlayson, Keith

AU - Dhaliwal, Kevin

AU - Stone, J. M.

N1 - Funding Information: The authors thank the BioResource for access to tissue (NHS Lothian BioResource, Scotland Research Ethics Service, reference 15/ES/0094). James M. Stone, Stephanos Yerolatsitis and Harry A. C. Wood are supported through an EPSRC fellowship (EP/S001123/1). Susan Fernandes is supported through an MRC fellowship (MR/R017794/1). Ahsan R. Akram is supported by a CRUK Clinician Scientist Fellowship (A24867).

PY - 2021/10/31

Y1 - 2021/10/31

N2 - Using the shifted-excitation Raman difference spectroscopy technique and an optical fibre featuring a negative curvature excitation core and a coaxial ring of high numerical aperture collection cores, we have developed a portable, background and fluorescence free, endoscopic Raman probe. The probe consists of a single fibre with a diameter of less than 0.25 mm packaged in a sub-millimetre tubing, making it compatible with standard bronchoscopes. The Raman excitation light in the fibre is guided in air and therefore interacts little with silica, enabling an almost background free transmission of the excitation light. In addition, we used the shifted-excitation Raman difference spectroscopy technique and a tunable 785 nm laser to separate the fluorescence and the Raman spectrum from highly fluorescent samples, demonstrating the suitability of the probe for biomedical applications. Using this probe we also acquired fluorescence free human lung tissue data.

AB - Using the shifted-excitation Raman difference spectroscopy technique and an optical fibre featuring a negative curvature excitation core and a coaxial ring of high numerical aperture collection cores, we have developed a portable, background and fluorescence free, endoscopic Raman probe. The probe consists of a single fibre with a diameter of less than 0.25 mm packaged in a sub-millimetre tubing, making it compatible with standard bronchoscopes. The Raman excitation light in the fibre is guided in air and therefore interacts little with silica, enabling an almost background free transmission of the excitation light. In addition, we used the shifted-excitation Raman difference spectroscopy technique and a tunable 785 nm laser to separate the fluorescence and the Raman spectrum from highly fluorescent samples, demonstrating the suitability of the probe for biomedical applications. Using this probe we also acquired fluorescence free human lung tissue data.

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Sub millimetre flexible fibre probe for background and fluorescence free Raman spectroscopy

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Fellowship - Next Generation Endoscopes

Dataset for "Sub millimetre flexible fibre probe for background and fluorescence free Raman spectroscopy"

Cite this

Sub millimetre flexible fibre probe for background and fluorescence free Raman spectroscopy

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Projects

Fellowship - Next Generation Endoscopes

Datasets

Dataset for "Sub millimetre flexible fibre probe for background and fluorescence free Raman spectroscopy"

Cite this