Sub millimetre flexible fibre probe for background and fluorescence free Raman spectroscopy

Stephanos Yerolatsitis, András Kufcsák, Katjana Ehrlich, Harry A.C. Wood, Susan Fernandes, Tom Quinn, Vikki Young, Irene Young, Katie Hamilton, Ahsan R. Akram, Robert R. Thomson, Keith Finlayson, Kevin Dhaliwal, J. M. Stone

Research output: Contribution to journalArticlepeer-review

4 Citations (SciVal)


Using the shifted-excitation Raman difference spectroscopy technique and an optical fibre featuring a negative curvature excitation core and a coaxial ring of high numerical aperture collection cores, we have developed a portable, background and fluorescence free, endoscopic Raman probe. The probe consists of a single fibre with a diameter of less than 0.25 mm packaged in a sub-millimetre tubing, making it compatible with standard bronchoscopes. The Raman excitation light in the fibre is guided in air and therefore interacts little with silica, enabling an almost background free transmission of the excitation light. In addition, we used the shifted-excitation Raman difference spectroscopy technique and a tunable 785 nm laser to separate the fluorescence and the Raman spectrum from highly fluorescent samples, demonstrating the suitability of the probe for biomedical applications. Using this probe we also acquired fluorescence free human lung tissue data.

Original languageEnglish
Article numbere202000488
JournalJournal of Biophotonics
Issue number10
Early online date15 Apr 2021
Publication statusPublished - 31 Oct 2021

Bibliographical note

Funding Information:
The authors thank the BioResource for access to tissue (NHS Lothian BioResource, Scotland Research Ethics Service, reference 15/ES/0094). James M. Stone, Stephanos Yerolatsitis and Harry A. C. Wood are supported through an EPSRC fellowship (EP/S001123/1). Susan Fernandes is supported through an MRC fellowship (MR/R017794/1). Ahsan R. Akram is supported by a CRUK Clinician Scientist Fellowship (A24867).


  • hollow-core fibre
  • Raman fibre probe
  • Raman spectroscopy

ASJC Scopus subject areas

  • General Chemistry
  • General Materials Science
  • General Biochemistry,Genetics and Molecular Biology
  • General Engineering
  • General Physics and Astronomy


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