Sub millimetre flexible fibre probe for background and fluorescence free Raman spectroscopy

Stephanos Yerolatsitis, András Kufcsák, Katjana Ehrlich, Harry A.C. Wood, Susan Fernandes, Tom Quinn, Vikki Young, Irene Young, Katie Hamilton, Ahsan R. Akram, Robert R. Thomson, Keith Finlayson, Kevin Dhaliwal, J. M. Stone

Research output: Contribution to journalArticlepeer-review


Using the shifted-excitation Raman difference spectroscopy technique and an optical fibre featuring a negative curvature excitation core and a coaxial ring of high numerical aperture collection cores, we have developed a portable, background and fluorescence free, endoscopic Raman probe. The probe consists of a single fibre with a diameter of less than 0.25 mm packaged in a sub-millimetre tubing, making it compatible with standard bronchoscopes. The Raman excitation light in the fibre is guided in air and therefore interacts little with silica, enabling an almost background free transmission of the excitation light. In addition, we used the shifted-excitation Raman difference spectroscopy technique and a tunable 785 nm laser to separate the fluorescence and the Raman spectrum from highly fluorescent samples, demonstrating the suitability of the probe for biomedical applications. Using this probe we also acquired fluorescence free human lung tissue data.

Original languageEnglish
Article numbere202000488
JournalJournal of Biophotonics
Early online date15 Apr 2021
Publication statusE-pub ahead of print - 15 Apr 2021


  • hollow-core fibre
  • Raman fibre probe
  • Raman spectroscopy

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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