Study of the attachment of Pseudomonas aeruginosa on gold and modified gold surfaces using surface plasmon resonance

A. Toby A. Jenkins; Richard Ffrench-Constant; Angus Buckling; David J. Clarke; Katherine Jarvis

doi:10.1021/bp034367u

Study of the attachment of Pseudomonas aeruginosa on gold and modified gold surfaces using surface plasmon resonance

A. Toby A. Jenkins, Richard Ffrench-Constant, Angus Buckling, David J. Clarke, Katherine Jarvis

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32 Citations (SciVal)

Abstract

This paper describes how the technique of surface plasmon resonance (SPR) can be utilized to follow (in real time) the attachment of Pseudomonas aeruginosa bacteria on bare gold and gold modified with a self-assembled monolayer (SAM) of mereaptounadecanoic acid. We show that SPR is able to discriminate between the adsorption of live versus dead (thermally shocked) bacteria. Moreover, the SPR distinguishes between the adsorption of wild-type versus mutant bacteria (single gene knockouts), the concentration of the bacterial suspension, and between bacteria adsorbing on SAM-modified and bare gold. SPR is able to measure bacterial adsorption within seconds of the bacterial suspension being introduced. Finally, a qualitative correlation between results from SPR with a crystal violet staining assay for different mutant bacteria was observed.

Original language	English
Pages (from-to)	1233-1236
Number of pages	4
Journal	Biotechnology Progress
Volume	20
Issue number	4
DOIs	https://doi.org/10.1021/bp034367u
Publication status	Published - 2004

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ID number: ISI:000223181000030

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title = "Study of the attachment of Pseudomonas aeruginosa on gold and modified gold surfaces using surface plasmon resonance",

abstract = "This paper describes how the technique of surface plasmon resonance (SPR) can be utilized to follow (in real time) the attachment of Pseudomonas aeruginosa bacteria on bare gold and gold modified with a self-assembled monolayer (SAM) of mereaptounadecanoic acid. We show that SPR is able to discriminate between the adsorption of live versus dead (thermally shocked) bacteria. Moreover, the SPR distinguishes between the adsorption of wild-type versus mutant bacteria (single gene knockouts), the concentration of the bacterial suspension, and between bacteria adsorbing on SAM-modified and bare gold. SPR is able to measure bacterial adsorption within seconds of the bacterial suspension being introduced. Finally, a qualitative correlation between results from SPR with a crystal violet staining assay for different mutant bacteria was observed.",

author = "Jenkins, {A. Toby A.} and Richard Ffrench-Constant and Angus Buckling and Clarke, {David J.} and Katherine Jarvis",

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T1 - Study of the attachment of Pseudomonas aeruginosa on gold and modified gold surfaces using surface plasmon resonance

AU - Jenkins, A. Toby A.

AU - Ffrench-Constant, Richard

AU - Buckling, Angus

AU - Clarke, David J.

AU - Jarvis, Katherine

N1 - ID number: ISI:000223181000030

PY - 2004

Y1 - 2004

N2 - This paper describes how the technique of surface plasmon resonance (SPR) can be utilized to follow (in real time) the attachment of Pseudomonas aeruginosa bacteria on bare gold and gold modified with a self-assembled monolayer (SAM) of mereaptounadecanoic acid. We show that SPR is able to discriminate between the adsorption of live versus dead (thermally shocked) bacteria. Moreover, the SPR distinguishes between the adsorption of wild-type versus mutant bacteria (single gene knockouts), the concentration of the bacterial suspension, and between bacteria adsorbing on SAM-modified and bare gold. SPR is able to measure bacterial adsorption within seconds of the bacterial suspension being introduced. Finally, a qualitative correlation between results from SPR with a crystal violet staining assay for different mutant bacteria was observed.

AB - This paper describes how the technique of surface plasmon resonance (SPR) can be utilized to follow (in real time) the attachment of Pseudomonas aeruginosa bacteria on bare gold and gold modified with a self-assembled monolayer (SAM) of mereaptounadecanoic acid. We show that SPR is able to discriminate between the adsorption of live versus dead (thermally shocked) bacteria. Moreover, the SPR distinguishes between the adsorption of wild-type versus mutant bacteria (single gene knockouts), the concentration of the bacterial suspension, and between bacteria adsorbing on SAM-modified and bare gold. SPR is able to measure bacterial adsorption within seconds of the bacterial suspension being introduced. Finally, a qualitative correlation between results from SPR with a crystal violet staining assay for different mutant bacteria was observed.

UR - http://dx.doi.org/10.1021/bp034367u

U2 - 10.1021/bp034367u

DO - 10.1021/bp034367u

M3 - Article

SN - 8756-7938

VL - 20

SP - 1233

EP - 1236

JO - Biotechnology Progress

JF - Biotechnology Progress

IS - 4

ER -

Study of the attachment of Pseudomonas aeruginosa on gold and modified gold surfaces using surface plasmon resonance

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