Study of the attachment of Pseudomonas aeruginosa on gold and modified gold surfaces using surface plasmon resonance

A. Toby A. Jenkins, Richard Ffrench-Constant, Angus Buckling, David J. Clarke, Katherine Jarvis

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

This paper describes how the technique of surface plasmon resonance (SPR) can be utilized to follow (in real time) the attachment of Pseudomonas aeruginosa bacteria on bare gold and gold modified with a self-assembled monolayer (SAM) of mereaptounadecanoic acid. We show that SPR is able to discriminate between the adsorption of live versus dead (thermally shocked) bacteria. Moreover, the SPR distinguishes between the adsorption of wild-type versus mutant bacteria (single gene knockouts), the concentration of the bacterial suspension, and between bacteria adsorbing on SAM-modified and bare gold. SPR is able to measure bacterial adsorption within seconds of the bacterial suspension being introduced. Finally, a qualitative correlation between results from SPR with a crystal violet staining assay for different mutant bacteria was observed.
Original languageEnglish
Pages (from-to)1233-1236
Number of pages4
JournalBiotechnology Progress
Volume20
Issue number4
DOIs
Publication statusPublished - 2004

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