Structures of native human thymidine phosphorylase and in complex with 5-iodouracil

E Mitsiki; A C Papageorgiou; Shalini Iyer; Nethaji Thiyagarajan; S H Prior; D Sleep; C Finnis; K Ravi Acharya

doi:10.1016/j.bbrc.2009.06.104

Structures of native human thymidine phosphorylase and in complex with 5-iodouracil

E Mitsiki, A C Papageorgiou, Shalini Iyer, Nethaji Thiyagarajan, S H Prior, D Sleep, C Finnis, K Ravi Acharya

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

44 Citations (SciVal)

Abstract

Thymidine phosphorylase (TP) first identified as platelet derived endothelial cell growth factor (PD-ECGF) plays a key role in nucleoside metabolism. Human TP (hTP) is implicated in angiogenesis and is overexpressed in several solid tumors. Here, we report the crystal structures of recombinant hTP and its complex with a substrate 5-iodouracil (5IUR) at 3.0 and 2.5 angstrom, respectively. in addition, we provide information on the role of specific residues in the enzymatic activity of hTP through mutagenesis and kinetic Studies.

Original language	English
Pages (from-to)	666-670
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	386
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2009.06.104
Publication status	Published - 4 Sept 2009

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10.1016/j.bbrc.2009.06.104

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abstract = "Thymidine phosphorylase (TP) first identified as platelet derived endothelial cell growth factor (PD-ECGF) plays a key role in nucleoside metabolism. Human TP (hTP) is implicated in angiogenesis and is overexpressed in several solid tumors. Here, we report the crystal structures of recombinant hTP and its complex with a substrate 5-iodouracil (5IUR) at 3.0 and 2.5 angstrom, respectively. in addition, we provide information on the role of specific residues in the enzymatic activity of hTP through mutagenesis and kinetic Studies.",

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AU - Mitsiki, E

AU - Papageorgiou, A C

AU - Iyer, Shalini

AU - Thiyagarajan, Nethaji

AU - Prior, S H

AU - Sleep, D

AU - Finnis, C

AU - Acharya, K Ravi

PY - 2009/9/4

Y1 - 2009/9/4

N2 - Thymidine phosphorylase (TP) first identified as platelet derived endothelial cell growth factor (PD-ECGF) plays a key role in nucleoside metabolism. Human TP (hTP) is implicated in angiogenesis and is overexpressed in several solid tumors. Here, we report the crystal structures of recombinant hTP and its complex with a substrate 5-iodouracil (5IUR) at 3.0 and 2.5 angstrom, respectively. in addition, we provide information on the role of specific residues in the enzymatic activity of hTP through mutagenesis and kinetic Studies.

AB - Thymidine phosphorylase (TP) first identified as platelet derived endothelial cell growth factor (PD-ECGF) plays a key role in nucleoside metabolism. Human TP (hTP) is implicated in angiogenesis and is overexpressed in several solid tumors. Here, we report the crystal structures of recombinant hTP and its complex with a substrate 5-iodouracil (5IUR) at 3.0 and 2.5 angstrom, respectively. in addition, we provide information on the role of specific residues in the enzymatic activity of hTP through mutagenesis and kinetic Studies.

UR - https://www.scopus.com/pages/publications/67650459996

UR - http://dx.doi.org/10.1016/j.bbrc.2009.06.104

U2 - 10.1016/j.bbrc.2009.06.104

DO - 10.1016/j.bbrc.2009.06.104

M3 - Article

SN - 0006-291X

VL - 386

SP - 666

EP - 670

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

Structures of native human thymidine phosphorylase and in complex with 5-iodouracil

Abstract

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