Structures of native human thymidine phosphorylase and in complex with 5-iodouracil

E Mitsiki, A C Papageorgiou, Shalini Iyer, Nethaji Thiyagarajan, S H Prior, D Sleep, C Finnis, K Ravi Acharya

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

Thymidine phosphorylase (TP) first identified as platelet derived endothelial cell growth factor (PD-ECGF) plays a key role in nucleoside metabolism. Human TP (hTP) is implicated in angiogenesis and is overexpressed in several solid tumors. Here, we report the crystal structures of recombinant hTP and its complex with a substrate 5-iodouracil (5IUR) at 3.0 and 2.5 angstrom, respectively. in addition, we provide information on the role of specific residues in the enzymatic activity of hTP through mutagenesis and kinetic Studies.
Original languageEnglish
Pages (from-to)666-670
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume386
Issue number4
DOIs
Publication statusPublished - 4 Sep 2009

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