TY - JOUR
T1 - Structures of native human thymidine phosphorylase and in complex with 5-iodouracil
AU - Mitsiki, E
AU - Papageorgiou, A C
AU - Iyer, Shalini
AU - Thiyagarajan, Nethaji
AU - Prior, S H
AU - Sleep, D
AU - Finnis, C
AU - Acharya, K Ravi
PY - 2009/9/4
Y1 - 2009/9/4
N2 - Thymidine phosphorylase (TP) first identified as platelet derived endothelial cell growth factor (PD-ECGF) plays a key role in nucleoside metabolism. Human TP (hTP) is implicated in angiogenesis and is overexpressed in several solid tumors. Here, we report the crystal structures of recombinant hTP and its complex with a substrate 5-iodouracil (5IUR) at 3.0 and 2.5 angstrom, respectively. in addition, we provide information on the role of specific residues in the enzymatic activity of hTP through mutagenesis and kinetic Studies.
AB - Thymidine phosphorylase (TP) first identified as platelet derived endothelial cell growth factor (PD-ECGF) plays a key role in nucleoside metabolism. Human TP (hTP) is implicated in angiogenesis and is overexpressed in several solid tumors. Here, we report the crystal structures of recombinant hTP and its complex with a substrate 5-iodouracil (5IUR) at 3.0 and 2.5 angstrom, respectively. in addition, we provide information on the role of specific residues in the enzymatic activity of hTP through mutagenesis and kinetic Studies.
UR - http://www.scopus.com/inward/record.url?scp=67650459996&partnerID=8YFLogxK
UR - http://dx.doi.org/10.1016/j.bbrc.2009.06.104
U2 - 10.1016/j.bbrc.2009.06.104
DO - 10.1016/j.bbrc.2009.06.104
M3 - Article
SN - 0006-291X
VL - 386
SP - 666
EP - 670
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -