Structures of engineered Clostridium botulinum neurotoxin derivatives

Geoffrey Masuyer, P Stancombe, J A Chaddock, K Ravi Acharya

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Targeted secretion inhibitors (TSIs) are a new class of engineered biopharmaceutical molecules derived from the botulinum neurotoxins (BoNTs). They consist of the metalloprotease light chain (LC) and translocation domain (Hn) of BoNT; they thus lack the native toxicity towards motor neurons but are able to target soluble N-ethylmaleimide-sensitive fusion protein attachment receptor (SNARE) proteins. These functional fragment (LHn) derivatives are expressed as single-chain proteins and require post-translational activation into di-chain molecules for function. A range of BoNT derivatives have been produced to demonstrate the successful use of engineered SNARE substrate peptides at the LC-Hn interface that gives these molecules self-activating capabilities. Alternatively, recognition sites for specific exoproteases can be engineered to allow controlled activation. Here, the crystal structures of three LHn derivatives are reported between 2.7 and 3.0 angstrom resolution. Two of these molecules are derivatives of serotype A that contain a SNARE peptide. Additionally, a third structure corresponds to LHn serotype B that includes peptide linkers at the exoprotease activation site. In all three cases the added engineered segments could not be modelled owing to disorder. However, these structures highlight the strong interactions holding the LHn fold together despite the inclusion of significant polypeptide sequences at the LCHn interface.
Original languageEnglish
Pages (from-to)1466-1472
Number of pages7
JournalActa Crystallographica Section F-Structural Biology and Crystallization Communications
Volume67
Issue number12
DOIs
Publication statusPublished - Dec 2011

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