Structural basis of multivalent galactose-based dendrimer recognition by human galectin-7

Sneha Ramaswamy, Mazen H. Sleiman, Geoffrey Masuyer, Cécile Arbez-Gindre, Maria Micha-Screttas, Theodora Calogeropoulou, Barry R. Steele, K. Ravi Acharya

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Galectins are evolutionarily conserved and ubiquitously present animal lectins with a high affinity for β-galactose-containing oligosaccharides. To date, 15 mammalian galectins have been identified. Their involvement in cell-cell and cell-matrix interactions has highlighted their importance in signal transduction and other intracellular processes. Human galectin-7 (hGal-7) is a 15 kDa proto type galectin that forms a dimer in solution and its involvement in the stimulation and development of tumour growth has been reported. Previously, we reported the crystal structure of hGal-7 and its complex with galactose and lactose which provided insight into its molecular recognition and detailed interactions. Here, we present newly obtained high-resolution structural data on carbohydrate-based dendrons in complex with hGal-7. Our crystallographic data reveal how multivalent ligands interact with and form cross-links with these galectin molecules. Understanding how these dendrimeric compounds interact with hGal-7 would help in the design of new tools to investigate the recognition of carbohydrates by lectins.

Original languageEnglish
Pages (from-to)372-387
Number of pages16
JournalFEBS Journal
Volume282
Issue number2
Early online date24 Nov 2014
DOIs
Publication statusE-pub ahead of print - 24 Nov 2014

Keywords

  • carbohydrate binding
  • dendrimers
  • galectin-7
  • lectin
  • multivalency

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Ramaswamy, S., Sleiman, M. H., Masuyer, G., Arbez-Gindre, C., Micha-Screttas, M., Calogeropoulou, T., Steele, B. R., & Acharya, K. R. (2014). Structural basis of multivalent galactose-based dendrimer recognition by human galectin-7. FEBS Journal, 282(2), 372-387. https://doi.org/10.1111/febs.13140