Abstract
Galectins are evolutionarily conserved and ubiquitously present animal lectins with a high affinity for β-galactose-containing oligosaccharides. To date, 15 mammalian galectins have been identified. Their involvement in cell-cell and cell-matrix interactions has highlighted their importance in signal transduction and other intracellular processes. Human galectin-7 (hGal-7) is a 15 kDa proto type galectin that forms a dimer in solution and its involvement in the stimulation and development of tumour growth has been reported. Previously, we reported the crystal structure of hGal-7 and its complex with galactose and lactose which provided insight into its molecular recognition and detailed interactions. Here, we present newly obtained high-resolution structural data on carbohydrate-based dendrons in complex with hGal-7. Our crystallographic data reveal how multivalent ligands interact with and form cross-links with these galectin molecules. Understanding how these dendrimeric compounds interact with hGal-7 would help in the design of new tools to investigate the recognition of carbohydrates by lectins.
Original language | English |
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Pages (from-to) | 372-387 |
Number of pages | 16 |
Journal | FEBS Journal |
Volume | 282 |
Issue number | 2 |
Early online date | 24 Nov 2014 |
DOIs | |
Publication status | E-pub ahead of print - 24 Nov 2014 |
Keywords
- carbohydrate binding
- dendrimers
- galectin-7
- lectin
- multivalency
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology