Abstract
Streptococcal pyrogenic exotoxin A (SpeA) is a superantigen produced by Streptococcns pyogenes and is associated with severe infections characterized by rash, hypotension, multiorgan failure and a high mortality rate. In this study, an allelic form of this toxin, SpeA1, was crystallized with four molecules in the crystallographic asymmetric unit and its crystal structure was determined at 2.6 Å resolution. The crystallographic R-factor was 19.4% (33,497 reflections) for 7031 protein atoms and 88 water molecules. The overall structure of SpeA1 is considerably similar to that of other prototype microbial superantigens, either of staphylococcal or streptococcal origin, but has greatest similarity to staphylococcal enterotoxin C (SEC). Based on structural and mutagenesis data, we have mapped several important residues on the toxin molecule, which are involved in the recognition of major histocompatibility complex (MHC) class II molecules and T-cell receptors. Also, the toxin appears to possess a potential zinc-binding site which may have implications in binding to particular MHC class II molecules. Finally, we propose models for SpeA1-MHC class II and SpeA1-T-cell receptor association and the relevance of this phenomenon to the superantigenic action of this toxin is considered.
Original language | English |
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Pages (from-to) | 9-21 |
Number of pages | 13 |
Journal | EMBO Journal |
Volume | 18 |
Issue number | 1 |
DOIs | |
Publication status | Published - 4 Jan 1999 |
Keywords
- Molecular recognition
- Protein crystallography
- Pyrogenic exotoxin
- Superantigen
- Zinc binding
ASJC Scopus subject areas
- General Neuroscience
- Molecular Biology
- General Biochemistry,Genetics and Molecular Biology
- General Immunology and Microbiology