Stress response decay with aging visualized using a dual-channel logic-based fluorescent probe

Jingye Tian; Donglei Shi; Yanhui Zhang; Xiaokang Li; Xinming Li; Hao Teng; Tony D. James; Jian Li; Yuan Guo

doi:10.1039/d1sc04162b

Stress response decay with aging visualized using a dual-channel logic-based fluorescent probe

Jingye Tian, Donglei Shi, Yanhui Zhang, Xiaokang Li, Xinming Li, Hao Teng, Tony D. James, Jian Li, Yuan Guo

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

31 Citations (SciVal)

Abstract

Diagnosing aging for preventative intervention generally relies on the tracking of aging biomarkers in the resting state. However, the static marker levels are insufficient to fully evaluate aging, particularly given that the stress response capacity (SRC) decay is currently viewed as a critical feature of aging. Therefore, we have developed a dual-channel fluorescent probe ROKS capable of the logic-based visualization of thiophenol (stressor) and HOCl (thiophenol-activated stress response product) in vivo, which provides a new strategy from the time dimension to precisely assess the SRC of individuals under stress using the dual-channel fluorescence ratio. Using ROKS we observed that the SRC of live cells decayed with senescence, and that a higher SRC was found for young vs. aged Caenorhabditis elegans. As such, our study offers a promising strategy for the fluorescence-guided diagnosis of aging and paves the way for accurate evaluation of the efficacy of anti-aging drugs.

Original language	English
Pages (from-to)	13483-13491
Number of pages	9
Journal	Chemical Science
Volume	12
Issue number	40
Early online date	17 Sept 2021
DOIs	https://doi.org/10.1039/d1sc04162b
Publication status	Published - 28 Oct 2021

Funding

We gratefully appreciate the financial support from the National Natural Science Foundation of China (21977082 and 22037002), the Natural Science Basic Research Plan for Distinguished Young Scholars in Shaanxi Province of China (2020JC-38) and the Technology Plan Project of Xi'an (GXYD18.1). T. D. J. wishes to thank the Royal Society for a Wolfson Research Merit Award. Y. G. thanks the University of Orleans in France for the invited professor position. We gratefully appreciate the nancial support from the National Natural Science Foundation of China (21977082 and 22037002), the Natural Science Basic Research Plan for Distinguished Young Scholars in Shaanxi Province of China (2020JC-38) and the Technology Plan Project of Xi'an (GXYD18.1). T. D. J. wishes to thank the Royal Society for a Wolfson Research Merit Award.

ASJC Scopus subject areas

General Chemistry

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Cite this

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title = "Stress response decay with aging visualized using a dual-channel logic-based fluorescent probe",

abstract = "Diagnosing aging for preventative intervention generally relies on the tracking of aging biomarkers in the resting state. However, the static marker levels are insufficient to fully evaluate aging, particularly given that the stress response capacity (SRC) decay is currently viewed as a critical feature of aging. Therefore, we have developed a dual-channel fluorescent probe ROKS capable of the logic-based visualization of thiophenol (stressor) and HOCl (thiophenol-activated stress response product) in vivo, which provides a new strategy from the time dimension to precisely assess the SRC of individuals under stress using the dual-channel fluorescence ratio. Using ROKS we observed that the SRC of live cells decayed with senescence, and that a higher SRC was found for young vs. aged Caenorhabditis elegans. As such, our study offers a promising strategy for the fluorescence-guided diagnosis of aging and paves the way for accurate evaluation of the efficacy of anti-aging drugs.",

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AU - Tian, Jingye

AU - Shi, Donglei

AU - Zhang, Yanhui

AU - Li, Xiaokang

AU - Li, Xinming

AU - Teng, Hao

AU - James, Tony D.

AU - Li, Jian

AU - Guo, Yuan

PY - 2021/10/28

Y1 - 2021/10/28

N2 - Diagnosing aging for preventative intervention generally relies on the tracking of aging biomarkers in the resting state. However, the static marker levels are insufficient to fully evaluate aging, particularly given that the stress response capacity (SRC) decay is currently viewed as a critical feature of aging. Therefore, we have developed a dual-channel fluorescent probe ROKS capable of the logic-based visualization of thiophenol (stressor) and HOCl (thiophenol-activated stress response product) in vivo, which provides a new strategy from the time dimension to precisely assess the SRC of individuals under stress using the dual-channel fluorescence ratio. Using ROKS we observed that the SRC of live cells decayed with senescence, and that a higher SRC was found for young vs. aged Caenorhabditis elegans. As such, our study offers a promising strategy for the fluorescence-guided diagnosis of aging and paves the way for accurate evaluation of the efficacy of anti-aging drugs.

AB - Diagnosing aging for preventative intervention generally relies on the tracking of aging biomarkers in the resting state. However, the static marker levels are insufficient to fully evaluate aging, particularly given that the stress response capacity (SRC) decay is currently viewed as a critical feature of aging. Therefore, we have developed a dual-channel fluorescent probe ROKS capable of the logic-based visualization of thiophenol (stressor) and HOCl (thiophenol-activated stress response product) in vivo, which provides a new strategy from the time dimension to precisely assess the SRC of individuals under stress using the dual-channel fluorescence ratio. Using ROKS we observed that the SRC of live cells decayed with senescence, and that a higher SRC was found for young vs. aged Caenorhabditis elegans. As such, our study offers a promising strategy for the fluorescence-guided diagnosis of aging and paves the way for accurate evaluation of the efficacy of anti-aging drugs.

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Stress response decay with aging visualized using a dual-channel logic-based fluorescent probe

Abstract

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ASJC Scopus subject areas

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