Stress response decay with aging visualized using a dual-channel logic-based fluorescent probe

Jingye Tian, Donglei Shi, Yanhui Zhang, Xiaokang Li, Xinming Li, Hao Teng, Tony D. James, Jian Li, Yuan Guo

Research output: Contribution to journalArticlepeer-review

19 Citations (SciVal)

Abstract

Diagnosing aging for preventative intervention generally relies on the tracking of aging biomarkers in the resting state. However, the static marker levels are insufficient to fully evaluate aging, particularly given that the stress response capacity (SRC) decay is currently viewed as a critical feature of aging. Therefore, we have developed a dual-channel fluorescent probe ROKS capable of the logic-based visualization of thiophenol (stressor) and HOCl (thiophenol-activated stress response product) in vivo, which provides a new strategy from the time dimension to precisely assess the SRC of individuals under stress using the dual-channel fluorescence ratio. Using ROKS we observed that the SRC of live cells decayed with senescence, and that a higher SRC was found for young vs. aged Caenorhabditis elegans. As such, our study offers a promising strategy for the fluorescence-guided diagnosis of aging and paves the way for accurate evaluation of the efficacy of anti-aging drugs.

Original languageEnglish
Pages (from-to)13483-13491
Number of pages9
JournalChemical Science
Volume12
Issue number40
Early online date17 Sept 2021
DOIs
Publication statusPublished - 28 Oct 2021

ASJC Scopus subject areas

  • General Chemistry

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