Strategies to Promote Chondrogenesis and Osteogenesis from Human Bone Marrow Cells and Articular Chondrocytes Encapsulated in Polysaccharide Templates

Jodie C Pound, David W Green, Julian B Chaudhuri, Stephen Mann, Helmtrud I Roach, Richard O C Oreffo

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The aim of this study was to synthesize functional in vitro and in vivo 3-dimensional (3D) constructs using a mix of human mesenchymal populations and articular chondrocytes encapsulated in biomineralized polysaccharide templates. Single-cell-type populations or mixts. of both cell types were encapsulated in alginate/chitosan and cultured within a rotating-bioreactor, perfused bioreactor system, or static conditions for 28 days. Within single cell-type populations, type II collagen immunopos. cells were present within lacunae in rotating-bioreactor capsules, with an increased proportion of metabolically active cells compared with perfused and static constructs. Biochem. anal. indicated significantly increased ( p < 0.05) DNA and protein in rotating-bioreactor conditions compared with perfused or static. However, in coculture samples, DNA and protein was significantly increased in static cultures owing to the formation of large regions of partially mineralized osteoid. This osteoid was found only in static cultures and when the ratio of human bone marrow cells to chondrocytes was 2:1 or, to a lesser extent, 5:1 ratio capsules. S.c. implantation of capsules into immunocompromised mice also showed optimal osteoid formation when the ratio was 2:1. The current studies demonstrate the pivotal role of robust 3D biomimetic microenvironments and indicate the potential to harness the interactions between different cell types to create specific tissues. [on SciFinder (R)]
Original languageEnglish
Pages (from-to)2789-2799
Number of pages11
JournalTissue Engineering
Volume12
Issue number10
Publication statusPublished - 2006

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Polysaccharides
Bioreactors
Bone
Cells
DNA
Proteins
Alginate
Biomimetics
Chitosan
Collagen
Tissue

Keywords

  • human bone marrow cell and articular chondrocyte encapsulated in alginate/chitosan proliferated and showed chondrogenesis well in rotating-bioreactor whereas coculture showed osteoid formation in static bioreactor and in mouse)
  • Bone (osteoid
  • human bone marrow cell and articular chondrocyte encapsulated in alginate/chitosan proliferated and showed chondrogenesis indicated by type II collagen immunopos. cell)
  • Cell differentiation (human bone marrow cell and articular chondrocyte encapsulated in alginate/chitosan proliferated and differentiated well in rotating-bioreactor whereas coculture showed osteoid formation in static bioreactor and in mouse)
  • Bone formation
  • Bone marrow
  • unclassified)
  • Chondrocyte (articular
  • Human (human bone marrow cell and articular chondrocyte encapsulated in alginate/chitosan proliferated and showed chondrogenesis well in rotating-bioreactor whereas coculture showed osteoid formation in static bioreactor and in mouse)
  • Encapsulation
  • BIOL (Biological study) (type I
  • BSU (Biological study
  • chondrogenesis osteogenesis bioreactor articular chondrocyte differentiation
  • Bioreactors
  • human bone marrow cell and articular chondrocyte encapsulated in alginate/chitosan coculture showed osteogenesis by osteoid formation in static but not rotating or perfused bioreactor and in mouse)
  • BIOL (Biological study) (type II
  • Cartilage formation
  • Collagens Role
  • human bone marrow cell and articular chondrocyte encapsulated in alginate/chitosan showed osteogenesis by osteoid formation in static bioreactor and in mouse as indicated by type I collagen immunopos. cell)

Cite this

Pound, J. C., Green, D. W., Chaudhuri, J. B., Mann, S., Roach, H. I., & Oreffo, R. O. C. (2006). Strategies to Promote Chondrogenesis and Osteogenesis from Human Bone Marrow Cells and Articular Chondrocytes Encapsulated in Polysaccharide Templates. Tissue Engineering, 12(10), 2789-2799.

Strategies to Promote Chondrogenesis and Osteogenesis from Human Bone Marrow Cells and Articular Chondrocytes Encapsulated in Polysaccharide Templates. / Pound, Jodie C; Green, David W; Chaudhuri, Julian B; Mann, Stephen; Roach, Helmtrud I; Oreffo, Richard O C.

In: Tissue Engineering, Vol. 12, No. 10, 2006, p. 2789-2799.

Research output: Contribution to journalArticle

Pound, JC, Green, DW, Chaudhuri, JB, Mann, S, Roach, HI & Oreffo, ROC 2006, 'Strategies to Promote Chondrogenesis and Osteogenesis from Human Bone Marrow Cells and Articular Chondrocytes Encapsulated in Polysaccharide Templates', Tissue Engineering, vol. 12, no. 10, pp. 2789-2799.
Pound, Jodie C ; Green, David W ; Chaudhuri, Julian B ; Mann, Stephen ; Roach, Helmtrud I ; Oreffo, Richard O C. / Strategies to Promote Chondrogenesis and Osteogenesis from Human Bone Marrow Cells and Articular Chondrocytes Encapsulated in Polysaccharide Templates. In: Tissue Engineering. 2006 ; Vol. 12, No. 10. pp. 2789-2799.
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AB - The aim of this study was to synthesize functional in vitro and in vivo 3-dimensional (3D) constructs using a mix of human mesenchymal populations and articular chondrocytes encapsulated in biomineralized polysaccharide templates. Single-cell-type populations or mixts. of both cell types were encapsulated in alginate/chitosan and cultured within a rotating-bioreactor, perfused bioreactor system, or static conditions for 28 days. Within single cell-type populations, type II collagen immunopos. cells were present within lacunae in rotating-bioreactor capsules, with an increased proportion of metabolically active cells compared with perfused and static constructs. Biochem. anal. indicated significantly increased ( p < 0.05) DNA and protein in rotating-bioreactor conditions compared with perfused or static. However, in coculture samples, DNA and protein was significantly increased in static cultures owing to the formation of large regions of partially mineralized osteoid. This osteoid was found only in static cultures and when the ratio of human bone marrow cells to chondrocytes was 2:1 or, to a lesser extent, 5:1 ratio capsules. S.c. implantation of capsules into immunocompromised mice also showed optimal osteoid formation when the ratio was 2:1. The current studies demonstrate the pivotal role of robust 3D biomimetic microenvironments and indicate the potential to harness the interactions between different cell types to create specific tissues. [on SciFinder (R)]

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KW - Collagens Role

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