Detection of multiple biomarkers for disease diagnosis or treatment monitoring has received a lot of attention due to their potential impact on clinical decision making. Electrochemical biosensors have become one of the preferred detection approaches, due to the simplicity of the accompanying instrumentation. This chapter will explore how electrochemical sensors can be utilized for detection of multiple analytes by integration of sensors into microfluidic microsystems. Some key fabrication technologies for such devices will be presented utilizing polymer microfabrication, paper-based approaches, and the use of printed circuit boards. Next, the use of electrode arrays will be presented along with some commercial platforms, outlining plausible paths towards a successful electrochemical multiplexed sensor. Novel approaches based on microbeads and various labels will then be introduced along with various strategies and technologies utilized to achieve ultrasensitive multiplexed detection.
|Title of host publication||Modern Techniques in Biosensors: Detection Methods and Commercial Aspects|
|Editors||Gorachand Dutta, Arindam Biswas, Amian Chakrabarti|
|Publication status||Acceptance date - 28 Jun 2020|
- Electrochemical biosensors
- Multiplex immunoassays
- Microfluidics integration
- Printed circuit board
- Electrode arrays
Zupancic, U., Rainbow, J., Flynn, C., Aidoo-Brown, J., Estrela, P., & Moschou, D. (Accepted/In press). Strategies for Multiplexed Electrochemical Sensor Development. In G. Dutta, A. Biswas, & A. Chakrabarti (Eds.), Modern Techniques in Biosensors: Detection Methods and Commercial Aspects Springer Nature.