Stem cell expansion in a fluidised bed bioreactor for accelerated osseointegration of bone substitute material

I Benzeval, I G Turner, M J Ellis

Research output: Contribution to conferencePoster

Abstract

The aim of this work is to enable the culturing of human stem cells within a porous bioceramic scaffold in a novel fluidised bed bioreactor. As such, the populated scaffold would be superior to the current synthetic bioceramic bone graft materials commercially available. The presence of a viable cell population will promote more rapid osseointegration thus accelerating the healing process. Particles of hydroxyapatite / tricalcium phosphate (4-6mm in diameter) have been fluidised successfully. The results combined with data from terminal velocity experiments and fundamental theory of fluidisation, have been used to develop a CFD model of the bioreactor in FLUENT. This model utilises a combination of the Eulerian multiphase model and the dense discrete phase model to calculate the velocity, pressure drop and shear stress within the bioreactor allowing control of its operation. Tests to establish the shear stress in relation to attachment and detachment of a cell line, using a purpose-built flow chamber, will enable the final design of the fluidised bed to be optimised in this FLUENT model. This analysis will inform the next stage of the project where suitable conditions will be derived to seed and culture a cell line within the fluidised bed. This model has the scope to be adapted to encompass the differing requirements of a range of cell lines in conjunction with bone graft material of different geometries, compositions and properties.
LanguageEnglish
StatusPublished - 5 Sep 2012
EventTERMIS - Vienna, Austria
Duration: 4 Sep 20128 Sep 2012

Conference

ConferenceTERMIS
CountryAustria
CityVienna
Period4/09/128/09/12

Fingerprint

Bioreactors
Stem cells
Bone
Cells
Bioceramics
Grafts
Scaffolds
Shear stress
Fluidization
Hydroxyapatite
Cell culture
Pressure drop
Seed
Computational fluid dynamics
Phosphates
Geometry
Chemical analysis
Experiments

Cite this

Benzeval, I., Turner, I. G., & Ellis, M. J. (2012). Stem cell expansion in a fluidised bed bioreactor for accelerated osseointegration of bone substitute material. Poster session presented at TERMIS, Vienna, Austria.

Stem cell expansion in a fluidised bed bioreactor for accelerated osseointegration of bone substitute material. / Benzeval, I; Turner, I G; Ellis, M J.

2012. Poster session presented at TERMIS, Vienna, Austria.

Research output: Contribution to conferencePoster

Benzeval I, Turner IG, Ellis MJ. Stem cell expansion in a fluidised bed bioreactor for accelerated osseointegration of bone substitute material. 2012. Poster session presented at TERMIS, Vienna, Austria.
Benzeval, I ; Turner, I G ; Ellis, M J. / Stem cell expansion in a fluidised bed bioreactor for accelerated osseointegration of bone substitute material. Poster session presented at TERMIS, Vienna, Austria.
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