Status of genomic imprinting in epigenetically distinct pluripotent stem cells

Bowen Sun, Mitsuteru Ito, Sasha Mendjan, Yoko Ito, I. Gabrielle M. Brons, Adele Murrell, Ludovic Vallier, Anne C. Ferguson-Smith, Roger A. Pedersen

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Mouse epiblast stem cells (EpiSCs) derived from postimplantation embryos are developmentally and functionally different from embryonic stem cells (ESCs) generated from blastocysts. EpiSCs require Activin A and FGF2 signaling for self-renewal, similar to human ESCs (hESCs), while mouse ESCs require LIF and BMP4. Unlike ESCs, EpiSCs have undergone X-inactivation, similar to the tendency of hESCs. The shared self-renewal and X-inactivation properties of EpiSCs and hESCs suggest that they have an epigenetic state distinct from ESCs. This hypothesis predicts that EpiSCs would have monoallelic expression of most imprinted genes, like that observed in hESCs. Here, we confirm this prediction. By contrast, we find that mouse induced pluripotent stem cells (iPSCs) tend to lose imprinting similar to mouse ESCs. These findings reveal that iPSCs have an epigenetic status associated with their pluripotent state rather than their developmental origin. Our results also reinforce the view that hESCs and EpiSCs are in vitro counterparts, sharing an epigenetic status distinct from ESCs and iPSCs.
Original languageEnglish
Pages (from-to)161-168
Number of pages8
JournalStem Cells
Volume30
Issue number2
DOIs
Publication statusPublished - Feb 2012

Keywords

  • Animals
  • Antigens, Differentiation
  • Cells, Cultured
  • DNA Methylation
  • Embryonic Stem Cells
  • Epigenesis, Genetic
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Genomic Imprinting
  • Germ Layers
  • Homeodomain Proteins
  • Humans
  • Induced Pluripotent Stem Cells
  • Mice
  • Octamer Transcription Factor-3
  • Pluripotent Stem Cells
  • Real-Time Polymerase Chain Reaction
  • SOXB1 Transcription Factors
  • Transcription Factors

Fingerprint Dive into the research topics of 'Status of genomic imprinting in epigenetically distinct pluripotent stem cells'. Together they form a unique fingerprint.

  • Cite this

    Sun, B., Ito, M., Mendjan, S., Ito, Y., Brons, I. G. M., Murrell, A., Vallier, L., Ferguson-Smith, A. C., & Pedersen, R. A. (2012). Status of genomic imprinting in epigenetically distinct pluripotent stem cells. Stem Cells , 30(2), 161-168. https://doi.org/10.1002/stem.793