Stabilization of the dystroglycan complex in Cajal bands of myelinating Schwann cells through plectin-mediated anchorage to vimentin filaments

Gernot Walko, Karl L Wögenstein, Lilli Winter, Irmgard Fischer, M Laura Feltri, Gerhard Wiche

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Previous studies have unmasked plectin, a uniquely versatile intermediate filament-associated cytolinker protein, to be essential for skin and skeletal muscle integrity. Different sets of isoforms of the protein were found to stabilize cells mechanically, regulate cytoskeletal dynamics, and serve as a scaffolding platform for signaling molecules. Here, we investigated whether a similar scenario prevails in myelinating Schwann cells. Using isoform-specific antibodies, the two plectin variants predominantly expressed in the cytoplasmic compartment (Cajal bands) of Schwann cells were identified as plectin (P)1 and P1c. Coimmunoprecipitation and immunolocalization experiments revealed complex formation of Cajal band plectin with β-dystroglycan, the core component of the dystrophin glycoprotein complex that in Schwann cells is crucial for the compartmentalization and stabilization of the myelin sheath. To study the functional implications of Schwann cell-specific plectin-β-dystroglycan interaction, we generated conditional (Schwann cell-restricted) plectin knockout mice. Ablation of plectin in myelinating Schwann cells (SCs) was found not to affect myelin sheath formation but to abrogate the tight association of the dystroglycan complex with the intermediate filament cytoskeleton. We show that the disruption of this association leads to the destabilization of the dystroglycan complex combined with increased myelin sheath deformations observed in the peripheral nerve during ageing of the animal.

Original languageEnglish
Pages (from-to)1274-87
Number of pages14
JournalGlia
Volume61
Issue number8
Early online date9 Jul 2013
DOIs
Publication statusPublished - 1 Aug 2013

Keywords

  • Animals
  • Cells, Cultured
  • Dystroglycans/metabolism
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Myelin Sheath/chemistry
  • Nerve Fibers, Myelinated/chemistry
  • Plectin/metabolism
  • Protein Binding/physiology
  • Schwann Cells/chemistry
  • Sciatic Nerve/chemistry
  • Vimentin/metabolism

Cite this

Stabilization of the dystroglycan complex in Cajal bands of myelinating Schwann cells through plectin-mediated anchorage to vimentin filaments. / Walko, Gernot; Wögenstein, Karl L; Winter, Lilli; Fischer, Irmgard; Feltri, M Laura; Wiche, Gerhard.

In: Glia, Vol. 61, No. 8, 01.08.2013, p. 1274-87.

Research output: Contribution to journalArticle

Walko, Gernot ; Wögenstein, Karl L ; Winter, Lilli ; Fischer, Irmgard ; Feltri, M Laura ; Wiche, Gerhard. / Stabilization of the dystroglycan complex in Cajal bands of myelinating Schwann cells through plectin-mediated anchorage to vimentin filaments. In: Glia. 2013 ; Vol. 61, No. 8. pp. 1274-87.
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AU - Wögenstein, Karl L

AU - Winter, Lilli

AU - Fischer, Irmgard

AU - Feltri, M Laura

AU - Wiche, Gerhard

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N2 - Previous studies have unmasked plectin, a uniquely versatile intermediate filament-associated cytolinker protein, to be essential for skin and skeletal muscle integrity. Different sets of isoforms of the protein were found to stabilize cells mechanically, regulate cytoskeletal dynamics, and serve as a scaffolding platform for signaling molecules. Here, we investigated whether a similar scenario prevails in myelinating Schwann cells. Using isoform-specific antibodies, the two plectin variants predominantly expressed in the cytoplasmic compartment (Cajal bands) of Schwann cells were identified as plectin (P)1 and P1c. Coimmunoprecipitation and immunolocalization experiments revealed complex formation of Cajal band plectin with β-dystroglycan, the core component of the dystrophin glycoprotein complex that in Schwann cells is crucial for the compartmentalization and stabilization of the myelin sheath. To study the functional implications of Schwann cell-specific plectin-β-dystroglycan interaction, we generated conditional (Schwann cell-restricted) plectin knockout mice. Ablation of plectin in myelinating Schwann cells (SCs) was found not to affect myelin sheath formation but to abrogate the tight association of the dystroglycan complex with the intermediate filament cytoskeleton. We show that the disruption of this association leads to the destabilization of the dystroglycan complex combined with increased myelin sheath deformations observed in the peripheral nerve during ageing of the animal.

AB - Previous studies have unmasked plectin, a uniquely versatile intermediate filament-associated cytolinker protein, to be essential for skin and skeletal muscle integrity. Different sets of isoforms of the protein were found to stabilize cells mechanically, regulate cytoskeletal dynamics, and serve as a scaffolding platform for signaling molecules. Here, we investigated whether a similar scenario prevails in myelinating Schwann cells. Using isoform-specific antibodies, the two plectin variants predominantly expressed in the cytoplasmic compartment (Cajal bands) of Schwann cells were identified as plectin (P)1 and P1c. Coimmunoprecipitation and immunolocalization experiments revealed complex formation of Cajal band plectin with β-dystroglycan, the core component of the dystrophin glycoprotein complex that in Schwann cells is crucial for the compartmentalization and stabilization of the myelin sheath. To study the functional implications of Schwann cell-specific plectin-β-dystroglycan interaction, we generated conditional (Schwann cell-restricted) plectin knockout mice. Ablation of plectin in myelinating Schwann cells (SCs) was found not to affect myelin sheath formation but to abrogate the tight association of the dystroglycan complex with the intermediate filament cytoskeleton. We show that the disruption of this association leads to the destabilization of the dystroglycan complex combined with increased myelin sheath deformations observed in the peripheral nerve during ageing of the animal.

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KW - Cells, Cultured

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KW - Mice, Knockout

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KW - Nerve Fibers, Myelinated/chemistry

KW - Plectin/metabolism

KW - Protein Binding/physiology

KW - Schwann Cells/chemistry

KW - Sciatic Nerve/chemistry

KW - Vimentin/metabolism

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JF - Glia

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