Specific, high-affinity binding sites for human luteinizing hormone (hLH) and human chorionic gonadotrophin (hCG) in Candida species

T A Bramley, G S Menzies, Robert J Williams, O S Kinsman, D J Adams

Research output: Contribution to journalArticle

Abstract

The presence of specific binding sites for [125I]-labelled hLH and hCG is described in Candida species. Binding was present in three strains of Candida albicans, and in Candida tropicalis, and was greatest in microsomes, though binding was also present in cytosol fractions. hLH and hCG mutually competed for these binding sites. Other hormones did not bind and did not compete for hLH binding sites. Scatchard plots showed two classes of binding sites, one with high affinity, low capacity and the other with lower affinity, high capacity binding in both microsomes and cytosol. This is the first report of specific binding sites for mammalian peptide hormones in a yeast.
LanguageEnglish
Pages1050-1056
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume167
Issue number3
DOIs
StatusPublished - 1990

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Candida
Chorionic Gonadotropin
Luteinizing Hormone
Binding Sites
Microsomes
Cytosol
Candida tropicalis
Peptide Hormones
Candida albicans
Yeast
Yeasts
Hormones

Cite this

Specific, high-affinity binding sites for human luteinizing hormone (hLH) and human chorionic gonadotrophin (hCG) in Candida species. / Bramley, T A; Menzies, G S; Williams, Robert J; Kinsman, O S; Adams, D J.

In: Biochemical and Biophysical Research Communications, Vol. 167, No. 3, 1990, p. 1050-1056.

Research output: Contribution to journalArticle

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AU - Adams, D J

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