TY - JOUR
T1 - Specific binding of radiolabeled Cry1Fa insecticidal protein from Bacillus thuringiensis to midgut sites in lepidopteran species
AU - Hernández-Rodríguez, Carmen Sara
AU - Hernández-Martínez, Patricia
AU - Van Rie, Jeroen
AU - Escriche, Baltasar
AU - Ferré, Juan
PY - 2012
Y1 - 2012
N2 - Cry1Fa insecticidal protein was successfully radiolabeled with (125)I-Na. Specific binding to brush border membrane vesicles was shown for the lepidopteran species Ostrinia nubilalis, Spodoptera frugiperda, Spodoptera exigua, Helicoverpa armigera, Heliothis virescens, and Plutella xylostella. Homologous competition assays were performed to obtain equilibrium binding parameters (K(d) [dissociation constant] and R(t) [concentration of binding sites]) for these six insect species.
AB - Cry1Fa insecticidal protein was successfully radiolabeled with (125)I-Na. Specific binding to brush border membrane vesicles was shown for the lepidopteran species Ostrinia nubilalis, Spodoptera frugiperda, Spodoptera exigua, Helicoverpa armigera, Heliothis virescens, and Plutella xylostella. Homologous competition assays were performed to obtain equilibrium binding parameters (K(d) [dissociation constant] and R(t) [concentration of binding sites]) for these six insect species.
UR - http://www.scopus.com/inward/record.url?scp=84864081540&partnerID=8YFLogxK
UR - http://dx.doi.org/10.1128/AEM.07591-11
U2 - 10.1128/AEM.07591-11
DO - 10.1128/AEM.07591-11
M3 - Article
C2 - 22447600
VL - 78
SP - 4048
EP - 4050
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
IS - 11
ER -