Specific binding of radiolabeled Cry1Fa insecticidal protein from Bacillus thuringiensis to midgut sites in lepidopteran species

Carmen Sara Hernández-Rodríguez, Patricia Hernández-Martínez, Jeroen Van Rie, Baltasar Escriche, Juan Ferré

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Cry1Fa insecticidal protein was successfully radiolabeled with (125)I-Na. Specific binding to brush border membrane vesicles was shown for the lepidopteran species Ostrinia nubilalis, Spodoptera frugiperda, Spodoptera exigua, Helicoverpa armigera, Heliothis virescens, and Plutella xylostella. Homologous competition assays were performed to obtain equilibrium binding parameters (K(d) [dissociation constant] and R(t) [concentration of binding sites]) for these six insect species.
Original languageEnglish
Pages (from-to)4048-4050
Number of pages3
JournalApplied and Environmental Microbiology
Volume78
Issue number11
DOIs
Publication statusPublished - 2012

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