Abstract
In this study we establish an efficient method for the regeneration for Boechera holboellii via somatic embryogenesis. Immature cotyledons from siliques of 4–6 month-old plants were cultured on MS medium supplemented with plant growth regulators (BA — 6-benzylaminopurine; NAA — α-naphthaleneacetic acid; TDZ — 1-phelyl-3-(1,2,3-thiadiazol-5-yl) urea: 2,4 D-2,4-dichlorophenoxy-acetic acid). A high frequency of embryogenic callus was produced after two weeks in culture. The somatic embryos were obtained with a frequency of 10% of explants on MS medium supplemented with 1.34 μM NAA + 8.87 μM BA and 2.68 μM NAA + 17.74 μM BA within 3 weeks in culture. The alternative regime of MS medium supplemented with 1.34 μM NAA + 4.44 μM BA produced somatic embryos at a frequency of 38%.
| Original language | English |
|---|---|
| Pages (from-to) | 301-307 |
| Number of pages | 7 |
| Journal | Acta Biologica Hungarica |
| Volume | 60 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Sept 2009 |
Keywords
- Boechera
- Brassicaceae
- somatic embryogenesis
- tissue culture
- apomixis