Somatic embryogenesis in apomict Boechera holboellii

K M Taskin; K Turgut; Roderick J Scott

doi:10.1556/ABiol.60.2009.3.7

Somatic embryogenesis in apomict Boechera holboellii

K M Taskin, K Turgut, Roderick J Scott

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

2 Citations (SciVal)

Abstract

In this study we establish an efficient method for the regeneration for Boechera holboellii via somatic embryogenesis. Immature cotyledons from siliques of 4–6 month-old plants were cultured on MS medium supplemented with plant growth regulators (BA — 6-benzylaminopurine; NAA — α-naphthaleneacetic acid; TDZ — 1-phelyl-3-(1,2,3-thiadiazol-5-yl) urea: 2,4 D-2,4-dichlorophenoxy-acetic acid). A high frequency of embryogenic callus was produced after two weeks in culture. The somatic embryos were obtained with a frequency of 10% of explants on MS medium supplemented with 1.34 μM NAA + 8.87 μM BA and 2.68 μM NAA + 17.74 μM BA within 3 weeks in culture. The alternative regime of MS medium supplemented with 1.34 μM NAA + 4.44 μM BA produced somatic embryos at a frequency of 38%.

Original language	English
Pages (from-to)	301-307
Number of pages	7
Journal	Acta Biologica Hungarica
Volume	60
Issue number	3
DOIs	https://doi.org/10.1556/ABiol.60.2009.3.7
Publication status	Published - Sept 2009

Keywords

Boechera
Brassicaceae
somatic embryogenesis
tissue culture
apomixis

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10.1556/ABiol.60.2009.3.7

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title = "Somatic embryogenesis in apomict Boechera holboellii",

abstract = "In this study we establish an efficient method for the regeneration for Boechera holboellii via somatic embryogenesis. Immature cotyledons from siliques of 4–6 month-old plants were cultured on MS medium supplemented with plant growth regulators (BA — 6-benzylaminopurine; NAA — α-naphthaleneacetic acid; TDZ — 1-phelyl-3-(1,2,3-thiadiazol-5-yl) urea: 2,4 D-2,4-dichlorophenoxy-acetic acid). A high frequency of embryogenic callus was produced after two weeks in culture. The somatic embryos were obtained with a frequency of 10% of explants on MS medium supplemented with 1.34 μM NAA + 8.87 μM BA and 2.68 μM NAA + 17.74 μM BA within 3 weeks in culture. The alternative regime of MS medium supplemented with 1.34 μM NAA + 4.44 μM BA produced somatic embryos at a frequency of 38%.",

keywords = "Boechera, Brassicaceae, somatic embryogenesis, tissue culture, apomixis",

author = "Taskin, {K M} and K Turgut and Scott, {Roderick J}",

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AU - Taskin, K M

AU - Turgut, K

AU - Scott, Roderick J

PY - 2009/9

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N2 - In this study we establish an efficient method for the regeneration for Boechera holboellii via somatic embryogenesis. Immature cotyledons from siliques of 4–6 month-old plants were cultured on MS medium supplemented with plant growth regulators (BA — 6-benzylaminopurine; NAA — α-naphthaleneacetic acid; TDZ — 1-phelyl-3-(1,2,3-thiadiazol-5-yl) urea: 2,4 D-2,4-dichlorophenoxy-acetic acid). A high frequency of embryogenic callus was produced after two weeks in culture. The somatic embryos were obtained with a frequency of 10% of explants on MS medium supplemented with 1.34 μM NAA + 8.87 μM BA and 2.68 μM NAA + 17.74 μM BA within 3 weeks in culture. The alternative regime of MS medium supplemented with 1.34 μM NAA + 4.44 μM BA produced somatic embryos at a frequency of 38%.

AB - In this study we establish an efficient method for the regeneration for Boechera holboellii via somatic embryogenesis. Immature cotyledons from siliques of 4–6 month-old plants were cultured on MS medium supplemented with plant growth regulators (BA — 6-benzylaminopurine; NAA — α-naphthaleneacetic acid; TDZ — 1-phelyl-3-(1,2,3-thiadiazol-5-yl) urea: 2,4 D-2,4-dichlorophenoxy-acetic acid). A high frequency of embryogenic callus was produced after two weeks in culture. The somatic embryos were obtained with a frequency of 10% of explants on MS medium supplemented with 1.34 μM NAA + 8.87 μM BA and 2.68 μM NAA + 17.74 μM BA within 3 weeks in culture. The alternative regime of MS medium supplemented with 1.34 μM NAA + 4.44 μM BA produced somatic embryos at a frequency of 38%.

KW - Boechera

KW - Brassicaceae

KW - somatic embryogenesis

KW - tissue culture

KW - apomixis

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DO - 10.1556/ABiol.60.2009.3.7

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JO - Acta Biologica Hungarica

JF - Acta Biologica Hungarica

IS - 3

ER -

Somatic embryogenesis in apomict Boechera holboellii

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