Solochrome cyanine

A histological stain for cobalt-chromium wear particles in metal-on-metal periprosthetic tissues

I Papadimitriou-Olivgeri, J. M. Brown, A. F. R. Kilpatrick, Harinderjit Gill, N. A. Athanasou

Research output: Contribution to journalArticle

Abstract

Metal-on-metal (MoM) hip arthroplasties produce abundant implant-derived wear debris composed mainly of cobalt (Co) and chromium (Cr). Cobalt-chromium (Co-Cr) wear particles are difficult to identify histologically and need to be distinguished from other wear particle types and endogenous components (e.g., haemosiderin, fibrin) which may be present in MoM periprosthetic tissues. In this study we sought to determine whether histological stains that have an affinity for metals are useful in identifying Co-Cr wear debris in MoM periprosthetic tissues. Histological sections of periprosthetic tissue from 30 failed MoM hip arthroplasties were stained with haematoxylin-eosin (HE), Solochrome Cyanine (SC), Solochrome Azurine (SA) and Perls' Prussian Blue (PB). Sections of periprosthetic tissue from 10 cases of non-MoM arthroplasties using other implant biomaterials, including titanium, ceramic, polymethylmethacrylate (PMMA) and ultra-high molecular weight polyethylene (UHMWP) were similarly analysed. Sections of 10 cases of haemosiderin-containing knee tenosynovial giant cell tumour (TSGCT) were also stained with HE, SC, SA and PB. In MoM periprosthetic tissues, SC stained metal debris in phagocytic macrophages and in the superficial necrotic zone which exhibited little or no trichrome staining for fibrin. In non-MoM periprosthetic tissues, UHMWP, PMMA, ceramic and titanium particles were not stained by SC. Prussian Blue, but not SC or SA, stained haemosiderin deposits in MoM periprosthetic tissues and TSGT. Our findings show that SC staining (most likely Cr-associated) is useful in distinguishing Co-Cr wear particles from other metal/non-metal wear particles types in histological preparations of periprosthetic tissue and that SC reliably distinguishes haemosiderin from Co-Cr wear debris.

Original languageEnglish
Article number103
Pages (from-to)1-8
Number of pages8
JournalJournal of Materials Science: Materials in Medicine
Volume30
Issue number9
DOIs
Publication statusPublished - 1 Sep 2019

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Cite this

Solochrome cyanine : A histological stain for cobalt-chromium wear particles in metal-on-metal periprosthetic tissues. / Papadimitriou-Olivgeri, I; Brown, J. M.; Kilpatrick, A. F. R.; Gill, Harinderjit; Athanasou, N. A.

In: Journal of Materials Science: Materials in Medicine, Vol. 30, No. 9, 103, 01.09.2019, p. 1-8.

Research output: Contribution to journalArticle

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