Snf2 controls pulcherriminic acid biosynthesis and antifungal activity of the biocontrol yeast Metschnikowia pulcherrima

Deborah Gore-Lloyd, Ines Sumann, Alexander Brachmann, Kerstin Schneeberger, Raul Ortiz-Merino, Mauro Moreno, Michael Schlafli, Pascal Kirner, Amanda Kron, Maria Rueda-Mejia, Vincent Somerville, Kenneth Wolfe, Jorn Piel, Christian Ahrens, Daniel Henk, Florian Freimoser

Research output: Contribution to journalArticle

Abstract

Metschnikowia pulcherrima synthesises the pigment pulcherrimin, from cyclodileucine (cyclo(Leu‐Leu)) as a precursor, and exhibits strong antifungal activity against notorious plant pathogenic fungi. This yeast therefore has great potential for biocontrol applications against fungal diseases; particularly in the phyllosphere where this species is frequently found. To elucidate the molecular basis of the antifungal activity of M. pulcherrima, we compared a wild‐type strain with a spontaneously occurring, pigmentless, weakly antagonistic mutant derivative. Whole genome sequencing of the wild‐type and mutant strains identified a point mutation that creates a premature stop codon in the transcriptional regulator gene SNF2 in the mutant. Complementation of the mutant strain with the wild‐type SNF2 gene restored pigmentation and recovered the strong antifungal activity. Mass spectrometry (UPLC HR HESI‐MS) proved the presence of the pulcherrimin precursors cyclo(Leu‐Leu) and pulcherriminic acid and identified new precursor and degradation products of pulcherriminic acid and/or pulcherrimin. All of these compounds were identified in the wild‐type and complemented strain, but were undetectable in the pigmentless snf2 mutant strain. These results thus identify Snf2 as a regulator of antifungal activity and pulcherriminic acid biosynthesis in M. pulcherrima and provide a starting point for deciphering the molecular functions underlying the antagonistic activity of this yeast.
LanguageEnglish
JournalMolecular Microbiology
Early online date12 May 2019
DOIs
StatusE-pub ahead of print - 12 May 2019

Cite this

Snf2 controls pulcherriminic acid biosynthesis and antifungal activity of the biocontrol yeast Metschnikowia pulcherrima. / Gore-Lloyd, Deborah; Sumann, Ines; Brachmann, Alexander; Schneeberger, Kerstin ; Ortiz-Merino, Raul; Moreno, Mauro; Schlafli, Michael; Kirner, Pascal; Kron, Amanda ; Rueda-Mejia, Maria; Somerville, Vincent ; Wolfe, Kenneth; Piel, Jorn; Ahrens, Christian ; Henk, Daniel; Freimoser, Florian.

In: Molecular Microbiology, 12.05.2019.

Research output: Contribution to journalArticle

Gore-Lloyd, D, Sumann, I, Brachmann, A, Schneeberger, K, Ortiz-Merino, R, Moreno, M, Schlafli, M, Kirner, P, Kron, A, Rueda-Mejia, M, Somerville, V, Wolfe, K, Piel, J, Ahrens, C, Henk, D & Freimoser, F 2019, 'Snf2 controls pulcherriminic acid biosynthesis and antifungal activity of the biocontrol yeast Metschnikowia pulcherrima', Molecular Microbiology. https://doi.org/10.1111/mmi.14272
Gore-Lloyd, Deborah ; Sumann, Ines ; Brachmann, Alexander ; Schneeberger, Kerstin ; Ortiz-Merino, Raul ; Moreno, Mauro ; Schlafli, Michael ; Kirner, Pascal ; Kron, Amanda ; Rueda-Mejia, Maria ; Somerville, Vincent ; Wolfe, Kenneth ; Piel, Jorn ; Ahrens, Christian ; Henk, Daniel ; Freimoser, Florian. / Snf2 controls pulcherriminic acid biosynthesis and antifungal activity of the biocontrol yeast Metschnikowia pulcherrima. In: Molecular Microbiology. 2019.
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abstract = "Metschnikowia pulcherrima synthesises the pigment pulcherrimin, from cyclodileucine (cyclo(Leu‐Leu)) as a precursor, and exhibits strong antifungal activity against notorious plant pathogenic fungi. This yeast therefore has great potential for biocontrol applications against fungal diseases; particularly in the phyllosphere where this species is frequently found. To elucidate the molecular basis of the antifungal activity of M. pulcherrima, we compared a wild‐type strain with a spontaneously occurring, pigmentless, weakly antagonistic mutant derivative. Whole genome sequencing of the wild‐type and mutant strains identified a point mutation that creates a premature stop codon in the transcriptional regulator gene SNF2 in the mutant. Complementation of the mutant strain with the wild‐type SNF2 gene restored pigmentation and recovered the strong antifungal activity. Mass spectrometry (UPLC HR HESI‐MS) proved the presence of the pulcherrimin precursors cyclo(Leu‐Leu) and pulcherriminic acid and identified new precursor and degradation products of pulcherriminic acid and/or pulcherrimin. All of these compounds were identified in the wild‐type and complemented strain, but were undetectable in the pigmentless snf2 mutant strain. These results thus identify Snf2 as a regulator of antifungal activity and pulcherriminic acid biosynthesis in M. pulcherrima and provide a starting point for deciphering the molecular functions underlying the antagonistic activity of this yeast.",
author = "Deborah Gore-Lloyd and Ines Sumann and Alexander Brachmann and Kerstin Schneeberger and Raul Ortiz-Merino and Mauro Moreno and Michael Schlafli and Pascal Kirner and Amanda Kron and Maria Rueda-Mejia and Vincent Somerville and Kenneth Wolfe and Jorn Piel and Christian Ahrens and Daniel Henk and Florian Freimoser",
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AU - Ortiz-Merino, Raul

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AU - Schlafli, Michael

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AU - Rueda-Mejia, Maria

AU - Somerville, Vincent

AU - Wolfe, Kenneth

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AU - Ahrens, Christian

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N2 - Metschnikowia pulcherrima synthesises the pigment pulcherrimin, from cyclodileucine (cyclo(Leu‐Leu)) as a precursor, and exhibits strong antifungal activity against notorious plant pathogenic fungi. This yeast therefore has great potential for biocontrol applications against fungal diseases; particularly in the phyllosphere where this species is frequently found. To elucidate the molecular basis of the antifungal activity of M. pulcherrima, we compared a wild‐type strain with a spontaneously occurring, pigmentless, weakly antagonistic mutant derivative. Whole genome sequencing of the wild‐type and mutant strains identified a point mutation that creates a premature stop codon in the transcriptional regulator gene SNF2 in the mutant. Complementation of the mutant strain with the wild‐type SNF2 gene restored pigmentation and recovered the strong antifungal activity. Mass spectrometry (UPLC HR HESI‐MS) proved the presence of the pulcherrimin precursors cyclo(Leu‐Leu) and pulcherriminic acid and identified new precursor and degradation products of pulcherriminic acid and/or pulcherrimin. All of these compounds were identified in the wild‐type and complemented strain, but were undetectable in the pigmentless snf2 mutant strain. These results thus identify Snf2 as a regulator of antifungal activity and pulcherriminic acid biosynthesis in M. pulcherrima and provide a starting point for deciphering the molecular functions underlying the antagonistic activity of this yeast.

AB - Metschnikowia pulcherrima synthesises the pigment pulcherrimin, from cyclodileucine (cyclo(Leu‐Leu)) as a precursor, and exhibits strong antifungal activity against notorious plant pathogenic fungi. This yeast therefore has great potential for biocontrol applications against fungal diseases; particularly in the phyllosphere where this species is frequently found. To elucidate the molecular basis of the antifungal activity of M. pulcherrima, we compared a wild‐type strain with a spontaneously occurring, pigmentless, weakly antagonistic mutant derivative. Whole genome sequencing of the wild‐type and mutant strains identified a point mutation that creates a premature stop codon in the transcriptional regulator gene SNF2 in the mutant. Complementation of the mutant strain with the wild‐type SNF2 gene restored pigmentation and recovered the strong antifungal activity. Mass spectrometry (UPLC HR HESI‐MS) proved the presence of the pulcherrimin precursors cyclo(Leu‐Leu) and pulcherriminic acid and identified new precursor and degradation products of pulcherriminic acid and/or pulcherrimin. All of these compounds were identified in the wild‐type and complemented strain, but were undetectable in the pigmentless snf2 mutant strain. These results thus identify Snf2 as a regulator of antifungal activity and pulcherriminic acid biosynthesis in M. pulcherrima and provide a starting point for deciphering the molecular functions underlying the antagonistic activity of this yeast.

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