Snapshot hyperspectral imaging of intracellular lasers

Soraya Caixeiro, Philip Wijesinghe, Kishan Dholakia, Malte c. Gather

Research output: Contribution to journalArticlepeer-review


Intracellular lasers are emerging as powerful biosensors for multiplexed tracking and precision sensing of cells and their microenvironment. This sensing capacity is enabled by quantifying their narrow-linewidth emission spectra, which is presently challenging to do at high speeds. In this work, we demonstrate rapid snapshot hyperspectral imaging of intracellular lasers. Using integral field mapping with a microlens array and a diffraction grating, we obtain images of the spatial and spectral intensity distribution from a single camera acquisition. We demonstrate widefield hyperspectral imaging over a 3 × 3 mm2 field of view and volumetric imaging over 250 × 250 × 800 µm3 (XYZ) volumes with a lateral (XY) resolution of 5 µm, axial (Z) resolution of 10 µm, and a spectral resolution of less than 0.8 nm. We evaluate the performance and outline the challenges and strengths of snapshot methods in the context of characterizing the emission from intracellular lasers. This method offers new opportunities for a diverse range of applications, including high-throughput and long-term biosensing with intracellular lasers.
Original languageEnglish
Article number33175
Number of pages16
JournalOptics Express
Issue number20
Publication statusPublished - 20 Sept 2023

Bibliographical note

Funding: Royal Commission for the Exhibition of 1851 (Research fellowship); Engineering and Physical Sciences Research Council (EP/P030017/1); Alexander von Humboldt-Stiftung (Humboldt professorship); Australian Research Council (FL210100099).

Data availability: The reconstruction code for SSHI is available at [42]. The data underpinning this work is available at [43].


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