siRNA for gene silencing: A route to drug target discovery

Simon W. Jones, Patricia M. de Souza, Mark A. Lindsay

Research output: Contribution to journalReview articlepeer-review

29 Citations (SciVal)


The identification of RNA interference in mammalian cells, mediated via both virally-derived short interference RNA (siRNA) and endogenously produced microRNA, has revolutionised our understanding of the translational control of gene expression. Indeed, since its initial discovery, siRNA has been rapidly deployed for the elucidation of gene function and the identification of potential drug targets, a process often known as target discovery. In this review, we briefly discuss the mechanism of RNA interference and then critically examine the use of siRNA in target discovery, with a particular emphasis upon issues such as efficacy, selectivity, delivery and application in high-throughput studies.

Original languageEnglish
Pages (from-to)522-527
Number of pages6
JournalCurrent Opinion in Pharmacology
Issue number5
Publication statusPublished - 31 Oct 2004

Bibliographical note

Funding Information:
Mark A Lindsay is supported by GlaxoSmithKline, and Patricia de Souza is supported by National Asthma Campaign (ID 03/070).

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery


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