Single-cell control of initial spatial structure in biofilm development using laser trapping

J.B. Hutchison, C.A. Rodesney, K.S. Kaushik, H.H. Le, D.A. Hurwitz, Y. Irie, V.D. Gordon

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Biofilms are sessile communities of microbes that are spatially structured by an embedding matrix. Biofilm infections are notoriously intractable. This arises, in part, from changes in the bacterial phenotype that result from spatial structure. Understanding these interactions requires methods to control the spatial structure of biofilms. We present a method for growing biofilms from initiating cells whose positions are controlled with single-cell precision using laser trapping. The native growth, motility, and surface adhesion of positioned microbes are preserved, as we show for model organisms Pseudomonas aeruginosa and Staphylococcus aureus. We demonstrate that laser-trapping and placing bacteria on surfaces can reveal the effects of spatial structure on bacterial growth in early biofilm development.
Original languageEnglish
Pages (from-to)4522-4530
Number of pages9
JournalLangmuir
Volume30
Issue number15
DOIs
Publication statusPublished - 22 Apr 2014

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biofilms
Biofilms
trapping
Lasers
cells
lasers
microorganisms
phenotype
pseudomonas
locomotion
staphylococcus
infectious diseases
organisms
embedding
bacteria
Bacteria
adhesion
Adhesion
matrices
interactions

Cite this

Hutchison, J. B., Rodesney, C. A., Kaushik, K. S., Le, H. H., Hurwitz, D. A., Irie, Y., & Gordon, V. D. (2014). Single-cell control of initial spatial structure in biofilm development using laser trapping. Langmuir, 30(15), 4522-4530. https://doi.org/10.1021/la500128y

Single-cell control of initial spatial structure in biofilm development using laser trapping. / Hutchison, J.B.; Rodesney, C.A.; Kaushik, K.S.; Le, H.H.; Hurwitz, D.A.; Irie, Y.; Gordon, V.D.

In: Langmuir, Vol. 30, No. 15, 22.04.2014, p. 4522-4530.

Research output: Contribution to journalArticle

Hutchison, JB, Rodesney, CA, Kaushik, KS, Le, HH, Hurwitz, DA, Irie, Y & Gordon, VD 2014, 'Single-cell control of initial spatial structure in biofilm development using laser trapping', Langmuir, vol. 30, no. 15, pp. 4522-4530. https://doi.org/10.1021/la500128y
Hutchison JB, Rodesney CA, Kaushik KS, Le HH, Hurwitz DA, Irie Y et al. Single-cell control of initial spatial structure in biofilm development using laser trapping. Langmuir. 2014 Apr 22;30(15):4522-4530. https://doi.org/10.1021/la500128y
Hutchison, J.B. ; Rodesney, C.A. ; Kaushik, K.S. ; Le, H.H. ; Hurwitz, D.A. ; Irie, Y. ; Gordon, V.D. / Single-cell control of initial spatial structure in biofilm development using laser trapping. In: Langmuir. 2014 ; Vol. 30, No. 15. pp. 4522-4530.
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