Simultaneous tracking of autophagy and oxidative stress during stroke with an ICT-TBET integrated ratiometric two-photon platform

Wei Hu; Taotao Qiang; Li Chai; Tianyu Liang; Longfang Ren; Fei Cheng; Chunya Li; Tony D. James

doi:10.1039/d1sc06805a

Simultaneous tracking of autophagy and oxidative stress during stroke with an ICT-TBET integrated ratiometric two-photon platform

Wei Hu, Taotao Qiang, Li Chai, Tianyu Liang, Longfang Ren, Fei Cheng, Chunya Li, Tony D. James

Research output: Contribution to journal › Article › peer-review

31 Citations (SciVal)

Abstract

Over recent years, fluorescent probes exhibiting simultaneous responses to multiple targets have been developed for in situ, real-time monitoring of cellular metabolism using two photon fluorescence sensing techniques due to numerous advantages including ease of operation, rapid reporting, high resolution, long visualization time and being non-invasive. However, due to interference from different fluorescence channels during simultaneous monitoring of multiple targets and the lack of ratiometric capability amongst the available probes, the accuracy in tracing metabolic processes has been restricted. With this research, using a through-bond energy transfer (TBET) mechanism, we designed a viscosity and peroxynitrite (ONOO⁻) mitochondria-targeting two-photon ratiometric fluorescent probe Mito-ONOO. Our results indicated that with decreasing levels of mitochondrial viscosity and increasing levels of ONOO⁻, the maximum of the emission wavelength of the probe shifted from 621 nm to 495 nm under 810 nm two-photon excitation. The baselines for the two emission peaks were significantly separated (Δλ = 126 nm), improving the resolution and reliability of bioimaging. Moreover, by ratiometric analysis during oxygen-glucose deprivation/reoxygenation (OGD/R, commonly used to simulate cell ischemia/reperfusion injury), the real-time visualization of the metabolic processes of autophagy and oxidative stress was possible. Our research indicated that during cellular oxygen-glucose deprivation/reoxygenation, cells produce ONOO⁻, causing cellular oxidative stress and cellular autophagy after 15 min, as such Mito-ONOO exhibits the potential for the monitoring and diagnosis of stroke, as well as providing insight into potential treatments, and drug design.

Original language	English
Pages (from-to)	5363-5373
Journal	Chemical Science
Volume	13
Issue number	18
Early online date	21 Apr 2022
DOIs	https://doi.org/10.1039/d1sc06805a
Publication status	Published - 14 May 2022

Bibliographical note

Funding Information:
This work was funded by the Open Project of Shaanxi Collaborative Innovation Center of Industrial Auxiliary Chemistry & Technology (No. XTKF-2020-05); The Key Scientific Research Group of Shaanxi Province (2020TD-009), Key Scientific Research Program of Shaanxi Provincial Education Department (Collaborative Innovation Center project) (20JY003), Science and Technology Plan Project of Xi'an Weiyang District (No. 201907) and the Youth Innovation Team of Shaanxi Universities. TDJ wishes to thank the Royal Society for a Wolfson Research Merit Award and the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University for support (2020ZD01). CYL wishes to thank the National Natural Science Foundation of China (No. 22074160, 22004133, 21874157, 21675175), Major Projects of Technical Innovation of Hubei Province (No. 2017ACA172) and Natural Science Foundation of Hubei Province (No. 2018CFB617). We thank Dr Xiaoxing Xiong and Dr Yinze Ye (Central Laboratory, Renmin Hospital of Wuhan University, China) for evaluation of histopathology, stroke pathological model construction and technical help.

ASJC Scopus subject areas

General Chemistry

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title = "Simultaneous tracking of autophagy and oxidative stress during stroke with an ICT-TBET integrated ratiometric two-photon platform",

abstract = "Over recent years, fluorescent probes exhibiting simultaneous responses to multiple targets have been developed for in situ, real-time monitoring of cellular metabolism using two photon fluorescence sensing techniques due to numerous advantages including ease of operation, rapid reporting, high resolution, long visualization time and being non-invasive. However, due to interference from different fluorescence channels during simultaneous monitoring of multiple targets and the lack of ratiometric capability amongst the available probes, the accuracy in tracing metabolic processes has been restricted. With this research, using a through-bond energy transfer (TBET) mechanism, we designed a viscosity and peroxynitrite (ONOO−) mitochondria-targeting two-photon ratiometric fluorescent probe Mito-ONOO. Our results indicated that with decreasing levels of mitochondrial viscosity and increasing levels of ONOO−, the maximum of the emission wavelength of the probe shifted from 621 nm to 495 nm under 810 nm two-photon excitation. The baselines for the two emission peaks were significantly separated (Δλ = 126 nm), improving the resolution and reliability of bioimaging. Moreover, by ratiometric analysis during oxygen-glucose deprivation/reoxygenation (OGD/R, commonly used to simulate cell ischemia/reperfusion injury), the real-time visualization of the metabolic processes of autophagy and oxidative stress was possible. Our research indicated that during cellular oxygen-glucose deprivation/reoxygenation, cells produce ONOO−, causing cellular oxidative stress and cellular autophagy after 15 min, as such Mito-ONOO exhibits the potential for the monitoring and diagnosis of stroke, as well as providing insight into potential treatments, and drug design.",

author = "Wei Hu and Taotao Qiang and Li Chai and Tianyu Liang and Longfang Ren and Fei Cheng and Chunya Li and James, {Tony D.}",

note = "Funding Information: This work was funded by the Open Project of Shaanxi Collaborative Innovation Center of Industrial Auxiliary Chemistry & Technology (No. XTKF-2020-05); The Key Scientific Research Group of Shaanxi Province (2020TD-009), Key Scientific Research Program of Shaanxi Provincial Education Department (Collaborative Innovation Center project) (20JY003), Science and Technology Plan Project of Xi'an Weiyang District (No. 201907) and the Youth Innovation Team of Shaanxi Universities. TDJ wishes to thank the Royal Society for a Wolfson Research Merit Award and the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University for support (2020ZD01). CYL wishes to thank the National Natural Science Foundation of China (No. 22074160, 22004133, 21874157, 21675175), Major Projects of Technical Innovation of Hubei Province (No. 2017ACA172) and Natural Science Foundation of Hubei Province (No. 2018CFB617). We thank Dr Xiaoxing Xiong and Dr Yinze Ye (Central Laboratory, Renmin Hospital of Wuhan University, China) for evaluation of histopathology, stroke pathological model construction and technical help. ",

year = "2022",

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T1 - Simultaneous tracking of autophagy and oxidative stress during stroke with an ICT-TBET integrated ratiometric two-photon platform

AU - Hu, Wei

AU - Qiang, Taotao

AU - Chai, Li

AU - Liang, Tianyu

AU - Ren, Longfang

AU - Cheng, Fei

AU - Li, Chunya

AU - James, Tony D.

N1 - Funding Information: This work was funded by the Open Project of Shaanxi Collaborative Innovation Center of Industrial Auxiliary Chemistry & Technology (No. XTKF-2020-05); The Key Scientific Research Group of Shaanxi Province (2020TD-009), Key Scientific Research Program of Shaanxi Provincial Education Department (Collaborative Innovation Center project) (20JY003), Science and Technology Plan Project of Xi'an Weiyang District (No. 201907) and the Youth Innovation Team of Shaanxi Universities. TDJ wishes to thank the Royal Society for a Wolfson Research Merit Award and the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University for support (2020ZD01). CYL wishes to thank the National Natural Science Foundation of China (No. 22074160, 22004133, 21874157, 21675175), Major Projects of Technical Innovation of Hubei Province (No. 2017ACA172) and Natural Science Foundation of Hubei Province (No. 2018CFB617). We thank Dr Xiaoxing Xiong and Dr Yinze Ye (Central Laboratory, Renmin Hospital of Wuhan University, China) for evaluation of histopathology, stroke pathological model construction and technical help.

PY - 2022/5/14

Y1 - 2022/5/14

N2 - Over recent years, fluorescent probes exhibiting simultaneous responses to multiple targets have been developed for in situ, real-time monitoring of cellular metabolism using two photon fluorescence sensing techniques due to numerous advantages including ease of operation, rapid reporting, high resolution, long visualization time and being non-invasive. However, due to interference from different fluorescence channels during simultaneous monitoring of multiple targets and the lack of ratiometric capability amongst the available probes, the accuracy in tracing metabolic processes has been restricted. With this research, using a through-bond energy transfer (TBET) mechanism, we designed a viscosity and peroxynitrite (ONOO−) mitochondria-targeting two-photon ratiometric fluorescent probe Mito-ONOO. Our results indicated that with decreasing levels of mitochondrial viscosity and increasing levels of ONOO−, the maximum of the emission wavelength of the probe shifted from 621 nm to 495 nm under 810 nm two-photon excitation. The baselines for the two emission peaks were significantly separated (Δλ = 126 nm), improving the resolution and reliability of bioimaging. Moreover, by ratiometric analysis during oxygen-glucose deprivation/reoxygenation (OGD/R, commonly used to simulate cell ischemia/reperfusion injury), the real-time visualization of the metabolic processes of autophagy and oxidative stress was possible. Our research indicated that during cellular oxygen-glucose deprivation/reoxygenation, cells produce ONOO−, causing cellular oxidative stress and cellular autophagy after 15 min, as such Mito-ONOO exhibits the potential for the monitoring and diagnosis of stroke, as well as providing insight into potential treatments, and drug design.

AB - Over recent years, fluorescent probes exhibiting simultaneous responses to multiple targets have been developed for in situ, real-time monitoring of cellular metabolism using two photon fluorescence sensing techniques due to numerous advantages including ease of operation, rapid reporting, high resolution, long visualization time and being non-invasive. However, due to interference from different fluorescence channels during simultaneous monitoring of multiple targets and the lack of ratiometric capability amongst the available probes, the accuracy in tracing metabolic processes has been restricted. With this research, using a through-bond energy transfer (TBET) mechanism, we designed a viscosity and peroxynitrite (ONOO−) mitochondria-targeting two-photon ratiometric fluorescent probe Mito-ONOO. Our results indicated that with decreasing levels of mitochondrial viscosity and increasing levels of ONOO−, the maximum of the emission wavelength of the probe shifted from 621 nm to 495 nm under 810 nm two-photon excitation. The baselines for the two emission peaks were significantly separated (Δλ = 126 nm), improving the resolution and reliability of bioimaging. Moreover, by ratiometric analysis during oxygen-glucose deprivation/reoxygenation (OGD/R, commonly used to simulate cell ischemia/reperfusion injury), the real-time visualization of the metabolic processes of autophagy and oxidative stress was possible. Our research indicated that during cellular oxygen-glucose deprivation/reoxygenation, cells produce ONOO−, causing cellular oxidative stress and cellular autophagy after 15 min, as such Mito-ONOO exhibits the potential for the monitoring and diagnosis of stroke, as well as providing insight into potential treatments, and drug design.

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Simultaneous tracking of autophagy and oxidative stress during stroke with an ICT-TBET integrated ratiometric two-photon platform

Abstract

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