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Abstract
The starch in waste bread (WB) from industrial sandwich production was directly converted to ethanol by an amylolytic, ethanologenic thermophile (Parageobacillus thermoglucosidasius strain TM333) under 5 different simultaneous saccharification and fermentation (SSF) regimes. Crude α-amylase from TM333 was used alone or in the presence of amyloglucosidase (AMG), a starch monomerizing enzyme used in industry, with/without prior gelatinisation/liquefaction treatments and P. thermoglucosidasius TM333 fermentation compared with Saccharomyces cerevisiae as a control. Results suggest that TM333 can ferment WB using SSF with yields of 94-100% of theoretical (based on all sugars in WB) in 48h without the need for AMG addition or any form of heat pre-treatment. This indicates that TM333 can transport and ferment all of the malto-oligosaccharides generated by its α-amylase. In the yeast control experiments, addition of AMG together with the crude α-amylase was necessary for full fermentation over the same time period. This suggests that industrial fermentation of WB starch to bio-ethanol or other products using an enhanced amylolytic P. thermoglucosidasius strain could offer significant cost savings compared to alternatives requiring enzyme supplementation.
Original language | English |
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Article number | 251 |
Journal | Microbial Cell Factories |
Volume | 21 |
Issue number | 1 |
Early online date | 27 Nov 2022 |
DOIs | |
Publication status | Published - 31 Dec 2022 |
Bibliographical note
Funding Information:The authors thank FoodWasteNet, a BBSRC Network in Industrial Biotechnology and Bioenergy, for funding, and Greencore UK for provision of materials.
Keywords
- Amyloglucosidase
- Bioethanol
- Gelatinisation
- Liquefaction
- Maltooligosaccharides
- Neopullulanase
- Parageobacillus thermoglucosidasius
- Saccharification
- Sandwich WB
- Simultaneous saccharification and fermentation SSF)
- ɑ-Amylase
- ɑ-Glucosidase
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
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Dive into the research topics of 'Simultaneous saccharification and ethanologenic fermentation (SSF) of waste bread by an amylolytic Parageobacillus thermoglucosidasius strain TM333'. Together they form a unique fingerprint.Projects
- 1 Finished
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Conversion of Waste Bread from Sandwich Industries to Bioethanol by Thermophilic Bacteria Capable of Utilizing Oligomeric Sugars
Leak, D. (PI)
Biotechnology and Biological Sciences Research Council
1/07/15 → 30/09/15
Project: Research council