Simultaneous saccharification and ethanologenic fermentation (SSF) of waste bread by an amylolytic Parageobacillus thermoglucosidasius strain TM333

David J. Leak, Christopher C. Ibenegbu

Research output: Contribution to journalArticlepeer-review

3 Citations (SciVal)

Abstract

The starch in waste bread (WB) from industrial sandwich production was directly converted to ethanol by an amylolytic, ethanologenic thermophile (Parageobacillus thermoglucosidasius strain TM333) under 5 different simultaneous saccharification and fermentation (SSF) regimes. Crude α-amylase from TM333 was used alone or in the presence of amyloglucosidase (AMG), a starch monomerizing enzyme used in industry, with/without prior gelatinisation/liquefaction treatments and P. thermoglucosidasius TM333 fermentation compared with Saccharomyces cerevisiae as a control. Results suggest that TM333 can ferment WB using SSF with yields of 94-100% of theoretical (based on all sugars in WB) in 48h without the need for AMG addition or any form of heat pre-treatment. This indicates that TM333 can transport and ferment all of the malto-oligosaccharides generated by its α-amylase. In the yeast control experiments, addition of AMG together with the crude α-amylase was necessary for full fermentation over the same time period. This suggests that industrial fermentation of WB starch to bio-ethanol or other products using an enhanced amylolytic P. thermoglucosidasius strain could offer significant cost savings compared to alternatives requiring enzyme supplementation.
Original languageEnglish
Article number251
JournalMicrobial Cell Factories
Volume21
Issue number1
Early online date27 Nov 2022
DOIs
Publication statusPublished - 31 Dec 2022

Bibliographical note

Funding Information:
The authors thank FoodWasteNet, a BBSRC Network in Industrial Biotechnology and Bioenergy, for funding, and Greencore UK for provision of materials.

Keywords

  • Amyloglucosidase
  • Bioethanol
  • Gelatinisation
  • Liquefaction
  • Maltooligosaccharides
  • Neopullulanase
  • Parageobacillus thermoglucosidasius
  • Saccharification
  • Sandwich WB
  • Simultaneous saccharification and fermentation SSF)
  • ɑ-Amylase
  • ɑ-Glucosidase

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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